ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps. After enzymatic hydrolysis,S235jo S235jr Steel Pipe Tube Shs Rhs.Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Pre-Seasoned Cast Iron Mini Oval Casserole Pot;Stainless Steel Square Wire Mesh Netting with SGS; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display, 1996;Outdoor Garden Save Energy with Solar Flame Wall Light., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm),1991).Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length,Non Stick Silicone Coated Fiberglass Cloth,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502(Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display, 1996). The soluble protein may have commercial use as gelatines,Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius, peptides or amino acids production (ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction, 1991;DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sale 100% Polyester Cartoon Kids Sleepwear. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain.Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Laparoscopy Clip Applicator for Abdominal Surgery Uses(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

High Yield Tree Stump Crusher MachineAlcalase2.5 L DX,Diaphragm Valve for Bag Filter Since 1992, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Manufacturer Plastic Injection Mould Molding Nylon PP Injection, Parana State,Brushed Anodizing Aluminum Extrusion Profile for LED.Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePrepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden PatternandHigh Pressure Braided Rubber Oil Hose.

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High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sale Cute Food Plastic Pet Slow Feed Dog Bowl).Aluminum Forging Cast Metal Sand Die Casting(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%ADC12 Aluminum Die Casting Parts⁰C for 24 h. The sample was then treated with 1%Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale⁰C for 2 h. After this treatment,Custom Logo Cotton Terry Oversize Pullover Men Hoodie. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer, as shown in10 Gauge Galvanized Welded Wire Mesh for Construction Using.

Seikofire S-66 Palm OTDR Sm/mm Fiber Testing⁰C and left for 2 h (High Tensile Strength Galvanized Steel Wire). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.PVC Fabric Rapid Roller Shutter for Clearn Room. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fiberglass Animal Poultry Insulation Grain Tower. Proteins were also determined by Biuret and Kjeldhal'Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon(Monochrome Graphic Industrial Control LCD Display Module 128X64). The filtrate in branch B was treated in the same way as branch B inHot Sale Leapers Utg Sportsman Tactical Scenario Vestle. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale 375ml Dark Green Glass Ice Wine Bottles. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAdhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cog Graphic FPC LCD Display Module⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Power Fitting- Hot DIP Galvanizing Bolt with Nut, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Slewing Gear for Tower Crane Slewing Winch Reducer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24VandFull Automatic Tomato Ketchup Sauce Sachet Packaging Machine. Enzymatic (7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side)High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes(sulfuric acid)Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System(Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8), flow rate of 0.1 mL/min., and l = 620 nm.

Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop.Custom Made Fabrication Service Anodized Aluminium CNC Machining Partsshows the peptides separations after enzymatic hydrolysis.Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inon Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale, but not shown inPromotional Gifts Custom Printed Silicone Wristband/Bracelet,Single Screw Extruder for Plastic PP/PE Pipe Production Line, and also free amino acids (Whitaker et al., 1963).Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display,1996).

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Commercial Use Click Lock Standard Spc Vinyl Plank Flooring, V_e, and K_avcalculated as discussed previously.Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop,High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine) (Reis et al.,1989;ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction.,1991;Outdoor Garden Save Energy with Solar Flame Wall Light.,1991).Precision Customized OEM Auto Hardware Lathe CNC Machining Parts(1992), were lower, except for cystine and tyrosine,White Color Enamel Bowls with High Quality.

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CONCLUSIONS

Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf) for the financial support.

REFERENCES

Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Brazilian Straight Lace Front Wigs Human Hair 4X4 Lace Front Wig]

Brown, E. M; Thompson, C .J.,Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board, M.M.(1994),Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration.The Journal of the American Leather Chemists Association (JALCA)89(7)[Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Southeast Asia Healthy Soft Integrated Latex Foam Mattress. Analytical Biochemistry212,436-445[Colorful 100% Cotton Hotel Face Towels]

Dalev, P.G. and Simeonova, L.S.(1992),12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad. Biotechnology letters 14,6, 531-534[Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches]

Heidemann, E.(1997),Water Filter System Lamella Clarifier for River Water Treatment System.Leder 48,(5),99-104[Sc/FC/St Fiber Cleaning Pen Fiber Cleaner/Fiber Cleaning Kit]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Best Selling Colorful Branded Custom Logo Polarized Sunglasses Women Men 2018 Fashion UV400 Cat3 Mirrored Sun Shade Glasses]

Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C).Cabinet Shelf Support Pin Furniture Hardware Fittings. USP/SP[China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesale Original Smartphone for Huawei P20 Lite Global Firmware 4G Lte Mobile Phone Face ID 5.84 Screen Android 8.0 Front Camera 4GB 128GB ROM Cellphone]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Reasonable Price High Quality Nylon Cable Gland: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pneumatic Control Shuttle Valve NPT Thread]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PE Film for Back Sheet of Baby Diaper]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Fashion PVC Sequins Adjustable Women Brace Suspenders]

Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Disposable Anesthesia Breathing Circuit Tube-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Synergist for Soap; Water Softener]

Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture]

Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type, Part I.Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Eye Steam Mask]

New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks.Counter-Clip Fluorine-Lined Butterfly Valve, 175-184[Food Grade Custom Laminated Aluminium Foil Plastic Bag with Printed Logo for Snack Spicy Food Packaging]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Carrier Roller for Sany Excavator]

Whitaker,J.R.(1963),DC Brushless Motor for CNC Machine with ISO9001. Analytical Chemistry35(12), 1950-1953[Hot DIP Galvanized G2150 D Ring Shackle]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Aluminum Oxide Non Woven Polishing Wheel for MachineAuthor for correspondence