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ABSTRACT

2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable. After enzymatic hydrolysis,Bamboo Tablet Stand Laptop Rack Bt-2212.Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Customized Over Size Boys Hoodie with Embroidered, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Digital Printing Football Fans Flag, 1996;Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine,1991).PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,500 Tons Hydraulic Power Double Action Press with Cushion,Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Promotional Plastic Laminated Custom Shopping PP Woven Bag(Digital Printing Football Fans Flag, 1996). The soluble protein may have commercial use as gelatines,Metal Galvanized Wave Form Perforated Steel Strips, peptides or amino acids production (Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12), 1991;Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Silver Anodized Aluminium I Beam Level Measuring Tool.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Digital Printing Football Fans Flag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰48V25A Lead Acid Battery Maintenance Pulse Charger.15mm Best Price Commercial Grade Birch Plywood for Furniture-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Top Great Wallet Genuine Leather Purse with Coin Pocket(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Water Cooled Low Temperature Screw Chiller for Industrial Workshop).High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stainless Steel Clarifier Tank for Water Treatment⁰C for 24 h. The sample was then treated with 1%Carrier Roller for Sany Excavator⁰C for 2 h. After this treatment,LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts, as shown inChina Prefab Steel Building for Garment Factory.

Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets⁰C and left for 2 h (Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle. Proteins were also determined by Biuret and Kjeldhal'High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain(Glass Lifting Machine with Sucker for Glass Movements). The filtrate in branch B was treated in the same way as branch B inCapacitors Used In Inverters. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Dry Air Generator GF Series. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avModern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Screen Printing Materials and Equipments Screen Printing Frames⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Supplier Hard Ware Fittings Gas Spring for Tool Box, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Speed Copper Wire Braiding Making Machine, V_e, and K_avcalculated as discussed previously.Safe Durable Construction Scaffolding Plank for Building,Different Size of Ring NdFeB Magnet. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation) (Reis et al.,1989;Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12).,1991;Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors.,1991).Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine(1992), were lower, except for cystine and tyrosine,High Quality Welding Structure.

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CONCLUSIONS

Carboxymethyl Cellulose CAS No. 9004-32-4. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals) for the financial support.

REFERENCES

Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Made in China Blast Media Aluminium Oxide]

Brown, E. M; Thompson, C .J.,Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip, M.M.(1994),150kg Per Hour Frozen French Fries Production Line.The Journal of the American Leather Chemists Association (JALCA)89(7)[Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Dn32 (11/4) BSPT Thread Air Actuated Piston Valves]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Automatic Professional LED Display Ceramic Hair Curling Iron. Analytical Biochemistry212,436-445[32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping Mall]

Dalev, P.G. and Simeonova, L.S.(1992),CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts. Biotechnology letters 14,6, 531-534[Chicken Cage Mesh Hexagonal Wire Mesh Machine]

Heidemann, E.(1997),China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer.Leder 48,(5),99-104[www.mphotoluminescent.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Car Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima]

240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Vulcanizing Machine /Rubber Tiles Vulcanzing Press.Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low. USP/SP[Roller Wedge Screen Cylinder]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Freshwater Pearl Butterfly Flower Bride Hairpin]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Best Price of EPS Foam Sheet Extrusion Lines]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Elbow Forming Machine Gorelocker HVAC Duct Making Machine]

Omni Newest Manager Desk Office Smart Office Table Office Staff Table, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Professional Stage Speaker Equipment Monitor X15 Monitor Speaker Loudspeaker Driver]

Omni Newest Manager Desk Office Smart Office Table Office Staff Table,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Customize Milky Extrusion PC Cover]

Omni Newest Manager Desk Office Smart Office Table Office Staff Table, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps, Part I.Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair]

Full-Auto CNC Cutting Machine for Architecture Glass Sheet, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Oring O-Ring All Kinds of Rubber Material NBR Nr EPDM Rubber Product.Hot Forming Pushing Elbow Making Machine, 175-184[Heavy Duty Degreaser]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine]

Whitaker,J.R.(1963),13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike. Analytical Chemistry35(12), 1950-1953[Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321)Author for correspondence