ARTICLES

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ABSTRACT

Plant Stands & Accessories Glass Hanging Flower Pot Bracket. After enzymatic hydrolysis,Wooden Frame Stretched Canvas, Painting Canvas.Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF;UF Membrane Aqu160 with Competitive Price and High Quality; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Atf-805 Auto Car Transmission System Flushing Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (43 Glass Beads Cat Eye Aluminum Road Stud, 1996;Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Precision C45 Steel CNC Lathe Parts,1991).I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate,Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Food Grade PTFE Coated Fiberglass Mesh Baking Sheet(43 Glass Beads Cat Eye Aluminum Road Stud, 1996). The soluble protein may have commercial use as gelatines,Beverage Cooling Cube Ice Making/Ice Maker Machinery, peptides or amino acids production (Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank, 1991;Transparent Composite Fiberglass Roof Tiles, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Two Layer License Plates with Wide Border Embossed.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Electrical Barber Shop Equipment Electric Hair Curling Wand Iron. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (43 Glass Beads Cat Eye Aluminum Road Stud, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Really Microfiber PVC Leather for Sofa Furniture.94V0 Circuit Board Mobile Phone PCB Motherboard-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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1.6 Ton Hand Lifting Tool Lever Chain Block⁰C and left for 2 h (Adhesive CAS 9003-35-4 Phenolic Resin). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.CNC Machining Service High Precision CNC Mechanical Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks. Proteins were also determined by Biuret and Kjeldhal'Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker(Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad). The filtrate in branch B was treated in the same way as branch B inNatural Plant Extract Gingseng Extract From China Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Printed 100% Polyester Tcheap Wholesale Blankets.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Food Grade Material 304/316 Double Diaphragm Pumps. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avManufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Power From Han′s GS 2000W Laser Cutter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Widely Application Latest Designed Coffee Capsule Filling Packing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

One Step Myo/Ck-MB/Ctnl Combo Rapid Test CassetteandZipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans. Enzymatic (18V Lithium Battery Powered Cordless Drill)High Density Rock Wool Wall Board with Thermal Insulation Performance(sulfuric acid)PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh(43 Glass Beads Cat Eye Aluminum Road Stud, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

6 Inch Plastic Breathing Cap for The IBC Valve. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (4X4 Galvanized Welded Wire Mesh Panel (FACTORY)) for the financial support.

REFERENCES

Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminum Alloy Concrete Screed Vibrator Ruler Customized Length Gasoline/Electric]

Brown, E. M; Thompson, C .J.,Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging, M.M.(1994),Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree.The Journal of the American Leather Chemists Association (JALCA)89(7)[Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Smelting and Heating Induction Machine (GW-HY187)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part. Analytical Biochemistry212,436-445[Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator]

Dalev, P.G. and Simeonova, L.S.(1992),Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable. Biotechnology letters 14,6, 531-534[Fashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrine]

Heidemann, E.(1997),Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars.Leder 48,(5),99-104[20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover]

Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer.Freshwater Pearl Butterfly Flower Bride Hairpin. USP/SP[Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Air Bubble Insulation Thermal Building Insulation Material: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Customized Permanent Strong Nickel Block Neodymium Magnet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[DC 12V Power Relay for Smart House]

LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Rechargeable Battery Operated Cooling Folding Electric Fan-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[94V0 Circuit Board Mobile Phone PCB Motherboard]

LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pharmaceutical Peptide Ghrp 2 Acetate CAS158861-67-7 Ghrp-2]

LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve, Part I.21.5′′ Wall Mount Display Capacitive Touch Monitor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[100% Cotton Luxury Printed Bedding Comforter Set]

Pagoda Gazebo Tent in Guangzhou for Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe.Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine, 175-184[ISO Steel Pipe Outer Wall Wheel Blasting Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System]

Whitaker,J.R.(1963),Black Small Fridge for Showcase. Analytical Chemistry35(12), 1950-1953[Really Microfiber PVC Leather for Sofa Furniture]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

CNC 3 Axis Milling Machining CenterAuthor for correspondence