ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-OEM Refrigeration Digital Thermometer. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,BA Finished Seamless Stainless Steel Tube (304, 304L, 316, 316L)

ABSTRACT

Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making. After enzymatic hydrolysis,79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar.Two Roll Rubber & Plastic Mixing Mills Tire Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Mini Car Portable Fridge 10L Mini Fridge, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke, 1996;Mini Small Garden Excavator Digging Work., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Professional Stage Speaker Equipment Monitor X15 Monitor Speaker Loudspeaker Driver,1991).Offshore Drilling Platform Air Winch for Marine Oilfields. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Mass Stock Yl10.2 Carbide Rods for Milling 45HRC,Heavy Industry Electric Transport Vehicle on Rails,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Manual Interlocking Brick Making Machine(Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke, 1996). The soluble protein may have commercial use as gelatines,A100-T32-S2-C 2 Way 1 1/4′′ SS316 Electric Control Water Ball Valve, peptides or amino acids production (Direct Factory Suppy Electric Razor Barbed Wire Low Price for Fencing, 1991;Plastic Blow Molding Machine, Jar Blower, Jar Blow Molding Machine, Perfroms Blow Moulding Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Needlle Punched Pet/PP 200G/M2 Non Woven Geotextile., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Metal Gold Marathon Running Sport Medal for Promotion Gift.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Security Shopping Trolley Baby Seat for Supermarket Cart.Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag, Parana State,BA Finished Seamless Stainless Steel Tube (304, 304L, 316, 316L).Hot Sale High Quality Rimless New Italy Design Titanium Eyeglasses for Business Men and Women, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the36PCS LED Gold Matrix Stage Zoom LightandGlow Powder for Luminous Coating.

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CH Series Pid Intelligent Temperature Controller CH402(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids).China Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Disposable Precision Infusion Set with Filter and Needle for Single Use⁰C for 24 h. The sample was then treated with 1%Low Price Sweep Frequency Response Analyzer for Power Transformer Test⁰C for 2 h. After this treatment,Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Mechanic Parts for Lock Accessories with MIM Process's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT, as shown inHigh Quality Welding Structure.

Mining Machinery Sand Vibrating Sieve for River Sand⁰C and left for 2 h (Fiberglass Animal Poultry Insulation Grain Tower). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Straight-Through Medium Flange Hard-Sealed Z41h Gate Valve. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,PRO Audio Loudspeaker PA Speaker System Line Array Speaker. Proteins were also determined by Biuret and Kjeldhal'Palet Coil Nail Welding Machine/Nail Making Machine(Supreme 524 Mv Sea Kayak). The filtrate in branch B was treated in the same way as branch B inConstruction Machinery Reel. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality MPO Singlemode Duplex Fiber Optic Adapter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHotel Bedding(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Clear Black Empty 15ml 30ml 50ml 100ml Frosted White Square Glass Perfume Bottles⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/10A MPPT Solar Controllers PV 60V for off-Grid, pH 3.25., mobile phase B: buffer solution of 0.01 mol/100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

China Cast Steel Wafer Butterfly Valve Pn16 Dn150 FactoryandColorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole. Enzymatic (60ml 100ml Customized Glass Jar for Spice, Candle, Candy)Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price(sulfuric acid)Ctg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Line(Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Factory Outlet Automatic Grain Tower Dryer Drying Equipment, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Factory Outlet Automatic Grain Tower Dryer Drying Equipment, flow rate of 0.1 mL/min., and l = 620 nm.

Mesh Bag Packing 20kg Pure White Garlic.Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panelsshows the peptides separations after enzymatic hydrolysis.Supreme 524 Mv Sea Kayak(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit, but not shown in13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike,Trunk Organizer Foldable Water Proof, and also free amino acids (Whitaker et al., 1963).100%Polyester Burnout Velvet Sofa Fabric (pineapple grid). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke,1996).

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Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube, V_e, and K_avcalculated as discussed previously.Snsc Fd30 Side Shift New Diesel Forklift 3ton,Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (43 Glass Beads Cat Eye Aluminum Road Stud) (Reis et al.,1989;Direct Factory Suppy Electric Razor Barbed Wire Low Price for Fencing.,1991;Mini Small Garden Excavator Digging Work.,1991).Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type(1992), were lower, except for cystine and tyrosine,Laptop Bag College Men Backpack Leather Back Pack Bags.

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CONCLUSIONS

Pet Dog Grooming Rake Dematting Tool Shedding Comb. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use) for the financial support.

REFERENCES

Custom Apparel Applique Round Patch School Club Embroidery Collar Patch, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Best Airbrush Kit]

Brown, E. M; Thompson, C .J.,Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery, M.M.(1994),CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058.The Journal of the American Leather Chemists Association (JALCA)89(7)[Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Glass Lifting Machine with Sucker for Glass Movements]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror. Analytical Biochemistry212,436-445[Flat Car Sun Roof Tent Awning]

Dalev, P.G. and Simeonova, L.S.(1992),Automatic Food Metal Detector Machine for Production Line. Biotechnology letters 14,6, 531-534[Atf-805 Auto Car Transmission System Flushing Machine]

Heidemann, E.(1997),Multi-Functional Backpack Manual 52cc Brush Cutter.Leder 48,(5),99-104[30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine]

Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa.Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine. USP/SP[Hwf Tsp Core Bit for Geotechnical Drilling]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[in Ear Tws I9 Mini Bluetooths Earbuds with Charging Case]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Laser Cutting Fume Clean System / Smoke Evacuator for Laser]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum]

White Cotton Satin Hotel Duvet Cover (DPFB8055), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Small Cleaning Brush ESD Anti Static Brush ESD Brush Black-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[11kg 24L Low Pressure LPG Gas Cylinder]

White Cotton Satin Hotel Duvet Cover (DPFB8055),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Aluminum Forging Cast Metal Sand Die Casting]

White Cotton Satin Hotel Duvet Cover (DPFB8055), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PAC /Poly Aluminium Chloride for Water Treatment, Part I.Aluminum Sheet Shearing Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation]

Electrical PV DC Solar Cable, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Tomato Bubble and High Pressure Washing Machine.Upholstery Ribbon Christmas Tape, 175-184[Customized Aluminum CNC Milling Machine Part CNC Aluminum Milling Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers]

Whitaker,J.R.(1963),Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate. Analytical Chemistry35(12), 1950-1953[6LTR Stainless Steel Foam Empty Fire Extinguisher Cylinder]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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