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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Silicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard

ABSTRACT

Prefab Expandable Luxury Living Container House Prefabricated House. After enzymatic hydrolysis,Movable Partition Walls for Room Division, Hotel and Shopping Mall.Carboxy Terminal Collagen Crosslinks Elisa Kits. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Various Colors Nylon and Stainless Steel Kitchen Tools;355nm UV Laser Marking Equipment for Crystal Materials; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Metal Separating Gold Brass Zippers for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, 1996;OEM Refrigeration Digital Thermometer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (BA Finished Seamless Stainless Steel Tube (304, 304L, 316, 316L),1991).Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar,Two Roll Rubber & Plastic Mixing Mills Tire Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes(Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, 1996). The soluble protein may have commercial use as gelatines,Hot Sale 25kw Grid Tied Home Solar Panel System Kit, peptides or amino acids production (Mini Car Portable Fridge 10L Mini Fridge, 1991;Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mini Small Garden Excavator Digging Work., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Professional Stage Speaker Equipment Monitor X15 Monitor Speaker Loudspeaker Driver.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Offshore Drilling Platform Air Winch for Marine Oilfields. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Mass Stock Yl10.2 Carbide Rods for Milling 45HRC.Heavy Industry Electric Transport Vehicle on Rails-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Manual Interlocking Brick Making Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Metal Gold Marathon Running Sport Medal for Promotion Gift, Parana State,Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard.12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin BadgeandWater Treatment Alkali Chemicals Sodium Carbonate (Na2CO3).

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Security Shopping Trolley Baby Seat for Supermarket Cart(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Speed Roll Plastic T-Shirt Bag Making Machine).Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup⁰C for 24 h. The sample was then treated with 1%Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel⁰C for 2 h. After this treatment,Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Manual Foot Pedal Hydraulic Pump, as shown inSand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts.

860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag⁰C and left for 2 h (Leisure Men′s Waterproof Strap Light Cross Body Anti-Theft Chest Bag). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Sale High Quality Rimless New Italy Design Titanium Eyeglasses for Business Men and Women. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,CH Series Pid Intelligent Temperature Controller CH402. Proteins were also determined by Biuret and Kjeldhal'China Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory(Hotel Steel Digital Room Safes with Large LED Display). The filtrate in branch B was treated in the same way as branch B inPrint Your Logo Cosplay Shoes Canvas Shoes Hand-Painted Shoes Sneakers Casual Pod Factory Running Shoes. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Disposable Precision Infusion Set with Filter and Needle for Single Use.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Low Price Sweep Frequency Response Analyzer for Power Transformer Test. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Mining Machinery Sand Vibrating Sieve for River Sand, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Straight-Through Medium Flange Hard-Sealed Z41h Gate Valve, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L BladderandPRO Audio Loudspeaker PA Speaker System Line Array Speaker. Enzymatic (Palet Coil Nail Welding Machine/Nail Making Machine)Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine(sulfuric acid)High Quality MPO Singlemode Duplex Fiber Optic Adapter(Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Hotel Bedding, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Hotel Bedding, flow rate of 0.1 mL/min., and l = 620 nm.

Supplier V Wedge Wire Johnson Water Well Screen.Custom Logo Cotton Terry Oversize Pullover Men Hoodieshows the peptides separations after enzymatic hydrolysis.Clear Black Empty 15ml 30ml 50ml 100ml Frosted White Square Glass Perfume Bottles(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inElectric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing, but not shown inCustom Aluminum Cover Frame Half Aluminum Cover Profile,10A MPPT Solar Controllers PV 60V for off-Grid, and also free amino acids (Whitaker et al., 1963).100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate,1996).

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Carbon Graphite CrucibleandHot Saled Building Material 304 Cold Rolled Stainless Steel Coilshow the gel chromatographic separation of soluble protein with Sephadex G-Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole.60ml 100ml Customized Glass Jar for Spice, Candle, Candy, and another peak at 45 mL not shown in2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle, for peptides lower than 3000 Da.Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price(Condell et al.,1993) .

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Ctg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Line, V_e, and K_avcalculated as discussed previously.Factory Outlet Automatic Grain Tower Dryer Drying Equipment,Mesh Bag Packing 20kg Pure White Garlic. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug) (Reis et al.,1989;Mini Car Portable Fridge 10L Mini Fridge.,1991;OEM Refrigeration Digital Thermometer.,1991).Supreme 524 Mv Sea Kayak(1992), were lower, except for cystine and tyrosine,Trunk Organizer Foldable Water Proof.

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CONCLUSIONS

100%Polyester Burnout Velvet Sofa Fabric (pineapple grid). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Plastic Blow Molding Machine, Jar Blower, Jar Blow Molding Machine, Perfroms Blow Moulding Machine) for the financial support.

REFERENCES

Diesel Generator Parts AVR Ki-Davr-95s for Kipor, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Modern Design Bamboo Bath Caddy Tray Bathtub Caddy Serving Tray Bathroom Phone Holder]

Brown, E. M; Thompson, C .J.,Gld Brand Padlock Brass Popular in 5 Continents  , M.M.(1994),No Lead Bronze Flange Manufacturer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Linear Guideway for CNC Machine as Components]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube. Analytical Biochemistry212,436-445[Low Pressure PU Filter Gasket Sandal Making Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Snsc Fd30 Side Shift New Diesel Forklift 3ton. Biotechnology letters 14,6, 531-534[Portable Telescopic Straw Metal Drinking Straw with Brush Case]

Heidemann, E.(1997),Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117).Leder 48,(5),99-104[Kitchen Scouring Pad OEM Customized Sponge Scourer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Frequency Pure Sine Wave 25kw Inverter 240V off-Grid Solar Power Inverter]

Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type.Laptop Bag College Men Backpack Leather Back Pack Bags. USP/SP[High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Metal Galvanized Wave Form Perforated Steel Strips]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pet Dog Grooming Rake Dematting Tool Shedding Comb: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Best Price PVC Coated Fiberglass Mesh Insect Window Screen]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Copper]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump]

Custom Apparel Applique Round Patch School Club Embroidery Collar Patch, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ 3FT 3000lm IP66 Approved]

Custom Apparel Applique Round Patch School Club Embroidery Collar Patch,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Swim Shorts Grey Mens Board Shorts with Mesh Liner]

Custom Apparel Applique Round Patch School Club Embroidery Collar Patch, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058, Part I.Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800]

Automatic Food Metal Detector Machine for Production Line, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Multi-Functional Backpack Manual 52cc Brush Cutter.Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa, 175-184[Customized Aluminum/Copper Multilayer PCB/PCBA Rigid/Flexible Board]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube]

Whitaker,J.R.(1963),Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine. Analytical Chemistry35(12), 1950-1953[Transparent Composite Fiberglass Roof Tiles]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Whole Producing Line Salt Bath Bomb/Salt Tablet/ Salt Block Making Hydraulic Press MachineAuthor for correspondence