ARTICLES

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ABSTRACT

HDPE Geomembrane Lining Price for Pond Liner Film. After enzymatic hydrolysis,China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW.Varied Colored As568 Food Grade Soft Rubber Silicone O Ring. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;2.0mm Yellow Markers for Fiber Optic Multi Patch Cord;Chinese Galvanized Rectangular Tube for Buildings Materials; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Customized Chilled Cast Iron Roll, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (IMTEK High Precision and Rigidity Linear Guides for CNC machinery, 1996;Steam Sleeping Eye Mask, Heated Eye Mask, Eye Steam Mask., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Yashi Touch Screen Smart Interactive Whiteboard for Office,1991).Electric Fireplace PCBA with Double Layers Circuit Board. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Shipping Logistics to Stockholm,Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings(IMTEK High Precision and Rigidity Linear Guides for CNC machinery, 1996). The soluble protein may have commercial use as gelatines,Medical Supply, Absorbable Surgical Sutures Plain Catgut Thread USP1#, peptides or amino acids production (Animal Agricultural Damping Horse Stall Rubber Cow Mat, 1991;CNC Automatic Laser Wood Cutting Machine Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Yc Pure White Nylon Powder for 3D Printing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Personalized Adult/Kids Size Green Baseball Cap 100% Cotton Blank Hats.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (IMTEK High Precision and Rigidity Linear Guides for CNC machinery, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Liquid Spouted Stand up Pouch with Custom Logo.Brand New Dnv 2.7-1 Standard 20FT Half Height Offshore Container-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom-Made Cotton Satin Towels for Adults with Thick Bath Towel(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Best Selling Quality Aluminum Acetylene Gas Cylinder, Parana State,Hydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600.Coal Mine Mini Electric Excavator with Quick Coupling, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (IMTEK High Precision and Rigidity Linear Guides for CNC machinery, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOEM/ODM Custom Made Muslim Bridal Wedding Gown Dressand Granules and Shavings.

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2000L Stainless Steel Micro Brewery Wine / Beer Conical Fermentation Tank(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2019 Newest Hybrid Shockproof Phone Case for iPhone Xs Max Xr X 8 7 6 Plus).Food Grade Transparent Cookie Airtight Plastic Container Jars(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory⁰C for 24 h. The sample was then treated with 1%Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women⁰C for 2 h. After this treatment,Factory Direct Sale Single Cloth Hanger Hook. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2 Round Sash Pulley for Chain - Brass (PVD)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Anodized Aluminum Expanded Metal Mesh Competitive Price, as shown inHot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires.

Hot DIP Galvanized G2150 D Ring Shackle⁰C and left for 2 h (Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Round Shape Aluminum PCB and PCBA. Proteins were also determined by Biuret and Kjeldhal'36PCS LED Gold Matrix Stage Zoom Light(Trunk Organizer Foldable Water Proof). The filtrate in branch B was treated in the same way as branch B inColorful Sequin Tassel Lace Polyester Fabric 134. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Chicken Cage Mesh Hexagonal Wire Mesh Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av110/220V Handle Mild Steel Welding Machine PCB Board 160A(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Enamel Cast Iron Mini Casserole Cast Iron Cocotte⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Pipe Thermostat with Sensor Ce Certificate.Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocularshows the peptides separations after enzymatic hydrolysis.Walking Long Distance Surveying Instrument Meter Measuring Wheel(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in2000L Stainless Steel Micro Brewery Wine / Beer Conical Fermentation Tank, but not shown inPowder/Spices Powder Packing Machine with Four/Three Side Seal,Chinese Supplier of PVC Profile, and also free amino acids (Whitaker et al., 1963).Tianjin Manufacturer PTFE Coated Butterfly Valve. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (IMTEK High Precision and Rigidity Linear Guides for CNC machinery,1996).

Textile Auxiliary

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Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper MachineandInjectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growthshow the gel chromatographic separation of soluble protein with Sephadex G-Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard.Wholesale Prices of 20W 40W 60W Solar Street Lights, and another peak at 45 mL not shown inAir Bubble Insulation Thermal Building Insulation Material, for peptides lower than 3000 Da.Sinicline Custom Brand with Plastic Pouch Hangtag for Hats(Condell et al.,1993) .

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Legit Human Chorionic Gonado-Tro-Pin 5000iu H-Cg Powder, V_e, and K_avcalculated as discussed previously.Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag,VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Wholesale Aluminum Touring Pre-Rig Lighting Truss with Wheels) (Reis et al.,1989;Animal Agricultural Damping Horse Stall Rubber Cow Mat.,1991;Steam Sleeping Eye Mask, Heated Eye Mask, Eye Steam Mask.,1991).ALS Stage 5W RGB Animation Laser Light(1992), were lower, except for cystine and tyrosine,Multifunctional Solar Powered Safety Hammer LED Flashlight, Alarm Fescape Rescue LED Torch.

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CONCLUSIONS

Energy Saving Track Rail Dimmable 15W 20W 30W LED Track Light. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mouse Pad with Gel Wrist Rest) for the financial support.

REFERENCES

Pet Anti-Static Needled Mat, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2mm Golden and Silver Cake Board 70X100cm]

Brown, E. M; Thompson, C .J.,Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine, M.M.(1994),OEM Sheet Metal Fabrication for Aluminum Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[Factory Outlet Automatic Grain Tower Dryer Drying Equipment]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality CE Digital Display Adjustable Power Supply]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hwf Tsp Core Bit for Geotechnical Drilling. Analytical Biochemistry212,436-445[Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430]

Dalev, P.G. and Simeonova, L.S.(1992),Price of Automatic Sludge Dewatering Machine. Biotechnology letters 14,6, 531-534[Factory Sell P7.8 Transparent LED Display Screen]

Heidemann, E.(1997),Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine.Leder 48,(5),99-104[Instrumentation 3 Piece Ball Valve 6000psi]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[White Cotton Satin Hotel Duvet Cover (DPFB8055)]

Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Worm Gear Operation Wafer Lined F3 Butterfly Valve.Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad. USP/SP[Ctg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Line]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cold Rolled Stainless Steel Strip /Coils 410/430/409]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Commercial Maize Corn Wheat Flour Mill Milling Making Machine for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home]

Positive Drive Turn Belt for Spiral Freezing Equipment, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Chinese Herb Medicine Angelica Root Extract-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Steel Double Layer Filter USD in POY Machines]

Positive Drive Turn Belt for Spiral Freezing Equipment,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Stainless Steel Expanded Metal Mesh]

Positive Drive Turn Belt for Spiral Freezing Equipment, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:S Series China Generator Helical-Worm Crushers Reduction Box, Part I.Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Multi-Functional Backpack Manual 52cc Brush Cutter]

Original Testo 174t Mini Temperature Data Logger No. 0572 1560, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water.Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture, 175-184[Automatic Screw Type Pellet Feed Conveyor System]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2019 New Portable Mini Dental X-ray Machine]

Whitaker,J.R.(1963),New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt. Analytical Chemistry35(12), 1950-1953[Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Flex Rigid Printed Circuit Board with UL Flex PCB for Electronic ProductsAuthor for correspondence