ARTICLES

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ABSTRACT

G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen. After enzymatic hydrolysis,Embossing Roller for Tissue Paper.Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates;Colorful  Reflective Sticker Reflective Tape; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Concrete Spare Part Electrical System Central Distribution Box, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress, 1996;50Hz to 60Hz Frequency Inverter for Water Pump., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330),1991).High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels,Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,PVC Cabinet Construction Board, White 18mm PVC Foam Board(Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress, 1996). The soluble protein may have commercial use as gelatines,2019 New Portable Mini Dental X-ray Machine, peptides or amino acids production (Ss Reduced Port 2000wog 1PC Screwed Ball Valve, 1991;China Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrous, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Prefab Steel Building for Garment Factory., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Dark Blue Color Texture Indoor Swimming Pool Glass Mosaic Tile.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cable Tray with ′t′ Slots. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰10 Gauge Galvanized Welded Wire Mesh for Construction Using.High Quality Factory Inline Hockey Skate Puck-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

201 301 304 Embossed Checkered Plate Stainless Steel Coil/SheetAlcalase2.5 L DX,Disposable PS Foam Fast Food Box Making Machine, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A)); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Foska Good Quality Plastic Stapler Set with Staples, Parana State,High Borosilicate Glass Baking Dish Set Clear.Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMetal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330)andLaser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls.

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Hotel SPA Swimming Pool, Sport Room, Bathroom Towel(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine).Powerful Cleaning Colorful Scouring Pad for Kitchen Use(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%500t Plywood Hot Press Woodworking Machine (12-25 layers)⁰C for 24 h. The sample was then treated with 1%10ml Round Clear Nail Polish Bottle with Brush Cap⁰C for 2 h. After this treatment,Full Automatic 4000b/h 500ml Plastic Jar Making Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)5L, 8L, 10L, 20L, 50L, 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine, as shown inPopular Water Bike / Hydro Bike / Aqua Bike (SK-8001).

PCB18h 8p8c Modular Plug with Shield Head RJ45 Connector⁰C and left for 2 h (100W Dimmable LED Corn Bulb with Meanwell Driver). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hermetic Sliding Lead Door, X Ray Room Door. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Laundry Used Gas Water Heater with CE/Asme Certificate. Proteins were also determined by Biuret and Kjeldhal'15W Aquarium Submersible UV Lamp Durable Germicidal Lamp(Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil). The filtrate in branch B was treated in the same way as branch B inChina Factory Big Capacity 500 Ml Honey Glass Bottle. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Accept OEM New Products Team Various Colors Gold Ribbon. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHnx Wholesale High Quality Metal Brass Standard Nutsert Lock Nut(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair, pH 3.25., mobile phase B: buffer solution of 0.01 mol/What′s Kind of Machine for Making Organic Fertilizer Granules, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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DC 12V Power Relay for Smart House.Gold Star Square Glass Candle Holder for Wedding Decorationshows the peptides separations after enzymatic hydrolysis.2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSolid Flex-Drain Pipe Length 40′, but not shown inHigh Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic,Medical Human Body Thermometer, and also free amino acids (Whitaker et al., 1963).Patent Product Solar Insect Killer Lamp, Made in Goldsun, China.. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress,1996).

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RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc, V_e, and K_avcalculated as discussed previously.48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads, Heavy Blanket, Gravity Blanket, Weighted Blanket,Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps) (Reis et al.,1989;Ss Reduced Port 2000wog 1PC Screwed Ball Valve.,1991;50Hz to 60Hz Frequency Inverter for Water Pump.,1991).Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52(1992), were lower, except for cystine and tyrosine,China Supplier Granular Coal Based Activated Carbon Filter Meida.

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CONCLUSIONS

Glass Cutting Table/CNC Glass Cutting Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A)) for the financial support.

REFERENCES

12 Bottles Portable Home Ues Thermoelectric Wine Chiller, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom CNC Sheet Metal Fabrication with Bending]

Brown, E. M; Thompson, C .J.,Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter, M.M.(1994),Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321).The Journal of the American Leather Chemists Association (JALCA)89(7)[Orange Monofilament Fishing Tackle Nylon Fish Net]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap. Analytical Biochemistry212,436-445[Good Quality Pillow for Pregnant Wome Body Pregnant Pillow Case]

Dalev, P.G. and Simeonova, L.S.(1992),Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening. Biotechnology letters 14,6, 531-534[Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3)]

Heidemann, E.(1997),Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle.Leder 48,(5),99-104[Low Price High Quality C Purlin Profile Roll Forming Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing]

Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe.Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger. USP/SP[OEM 15PCS Electric Face Makeup Brush with Ce Certificate]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Flexible Pvc Film]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Condition Hot Sale Type Air Cooled Ice Cube Maker]

Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Washami Teeth Care for Whitening Cool Mint Toothpaste]

Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb]

Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt, Part I.Hanwha SMT Chip Shooter LED Pick and Place Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Price of Automatic Sludge Dewatering Machine]

ACP Aluminium Cmposite Panel Cladding Extrusions, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine.Wholesale Custom Mens Heated Down Jacket, 175-184[Sports Bluetooth Earphone Tws Bluetooth Earbuds in-Ear Wireless Stereo Headset]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[D Section Stainless Steel Tube]

Whitaker,J.R.(1963),Rectification Triming Metallized Polyester Film Slitting Rewinding Machine. Analytical Chemistry35(12), 1950-1953[Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Cotton Yarn Computerized Lace Knitting MachineAuthor for correspondence