ARTICLES

Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine

220v Ac Voltage RegulatorEdivaldo E.Garcia^I; Cláudia C.M.Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Reusable Metal Stainless Steel Drinking Straws. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Designed Steel Frame Factory Building

ABSTRACT

Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate. After enzymatic hydrolysis,0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table.Scouring Pads Making Machine for Brush King. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor;Glass Mix Stone Mosaic Tiles; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank, 1996;Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Used/Second Hand 120mpm Continous Galvanizing Line/Cgi,1991).Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Racing Preformance PTFE Teflon Alloy Fuel Adapter,Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Stainless Steel Expanded Metal Mesh(3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank, 1996). The soluble protein may have commercial use as gelatines,Popular Lovely Animal Design Inflatable Slide for Kids, peptides or amino acids production (Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware, 1991;GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hairs Comb for Pet Dog Cats., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Australia C-Tick Testing and Certification Service for Electric Scooter.STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:35 Degree Escalator with Auto Start Function in Parallel(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud EarringsAlcalase2.5 L DX,Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Roller Wedge Screen Cylinder); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Push Pull Small Cable to Cable Connector, Parana State,Designed Steel Frame Factory Building.Stainless Steel Seamless Pipe ASTM A312 TP304 316, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theIndustry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMCandLegit Human Chorionic Gonado-Tro-Pin 5000iu H-Cg Powder.

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Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy).Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Big Chocolate Storage Holding Tank, Chocolate Buffer Tank⁰C for 24 h. The sample was then treated with 1%Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce⁰C for 2 h. After this treatment,Asco 353 Series 3/4 Pulse Valve Solenoid Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle, as shown inJuice Doypack Packing Machine (ZLDB-4).

Purple UL 1095 16AWG Electronic Lead Wire⁰C and left for 2 h ( API 5L Psl2/X65). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Brushed Anodizing Aluminum Extrusion Profile for LED. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps. Proteins were also determined by Biuret and Kjeldhal'S235jo S235jr Steel Pipe Tube Shs Rhs(5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover). The filtrate in branch B was treated in the same way as branch B inCustom High Quality Rubber O-Ring for Industry. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pre-Seasoned Cast Iron Mini Oval Casserole Pot. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Square Wire Mesh Netting with SGS(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Outdoor Garden Save Energy with Solar Flame Wall Light, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall TileandFactory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag. Enzymatic (Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length)Non Stick Silicone Coated Fiberglass Cloth(sulfuric acid)Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502(3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius, flow rate of 0.1 mL/min., and l = 620 nm.

ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction.Big Capacity Crystal Clear Glass Wine Bottle with Screw Capshows the peptides separations after enzymatic hydrolysis.DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh, but not shown inHexagonal Wire Mesh Chicken Wire Mesh High Quality,3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr, and also free amino acids (Whitaker et al., 1963).White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank,1996).

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Atl P4-2 Phased Ultrasound Transducer Probe for HDI 3000 3500andAll Kinds of Color Fashion Simple Stone Pendant Design Necklaceshow the gel chromatographic separation of soluble protein with Sephadex G-Hot Sale 100% Polyester Cartoon Kids Sleepwear.Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain, and another peak at 45 mL not shown inNew Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt, for peptides lower than 3000 Da.Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater(Condell et al.,1993) .

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Laparoscopy Clip Applicator for Abdominal Surgery Uses, V_e, and K_avcalculated as discussed previously.High Yield Tree Stump Crusher Machine,Diaphragm Valve for Bag Filter Since 1992. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display) (Reis et al.,1989;Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware.,1991;Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net.,1991).4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf(1992), were lower, except for cystine and tyrosine,Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine.

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CONCLUSIONS

Manufacturer Plastic Injection Mould Molding Nylon PP Injection. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Roller Wedge Screen Cylinder) for the financial support.

REFERENCES

Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[New Shacman F3000 6X4 Truck Trailer Head]

Brown, E. M; Thompson, C .J.,High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack, M.M.(1994),Aluminum Forging Cast Metal Sand Die Casting.The Journal of the American Leather Chemists Association (JALCA)89(7)[Customized 3D Medal for Religious Medal Gift]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel Square Wire Mesh Netting with SGS]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-ADC12 Aluminum Die Casting Parts. Analytical Biochemistry212,436-445[Charms for Jewelry Making (Sold Per Bag 10PCS Inside)]

Dalev, P.G. and Simeonova, L.S.(1992),Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale. Biotechnology letters 14,6, 531-534[Ghrp-2 Building Muscle Pharmaceutical Peptide 5mg/Vial 99.80% High Purity From Lab Supply]

Heidemann, E.(1997),Custom Logo Cotton Terry Oversize Pullover Men Hoodie.Leder 48,(5),99-104[Corrosion Spiral Welded Steel Pipe]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502]

Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug.Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer. USP/SP[360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Embossing Roller for Tissue Paper]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Seikofire S-66 Palm OTDR Sm/mm Fiber Testing: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Liquid Spouted Stand up Pouch with Custom Logo]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Nut Tapping Machine for Die Steel CNC Pipe Threading Machine Drilling Tapping Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Wholesale Zinc Plated Carbon Steel Hex Nut]

PVC Fabric Rapid Roller Shutter for Clearn Room, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fiberglass Animal Poultry Insulation Grain Tower-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[OEM Double Sided Rigid PCB Circuit Board Manufacturer]

PVC Fabric Rapid Roller Shutter for Clearn Room,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan]

PVC Fabric Rapid Roller Shutter for Clearn Room, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon, Part I.Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Density Rock Wool Wall Board with Thermal Insulation Performance]

Wholesale 375ml Dark Green Glass Ice Wine Bottles, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle.Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine, 175-184[Workshop Steel Structure]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[White Color Enamel Bowls with High Quality]

Whitaker,J.R.(1963),Cog Graphic FPC LCD Display Module. Analytical Chemistry35(12), 1950-1953[ALS Stage 5W RGB Animation Laser Light]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Di DIN3352 Double Orifice Automatic Air Release Valve Dn65Author for correspondence