ARTICLES

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ABSTRACT

Factory Supplied High Strength Marine Anchor Chain. After enzymatic hydrolysis,Humidity Refrigeration Digital Controller Jsd-100+.Elegant Europe Dessign Pillow Top Mattress. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8);Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Modern 4 Person Workstation with Computer Desk Modular Office Furniture, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill, 1996;Women Fashion Winter V Neck Long Sleeve Clothes., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line,1991).3D CNC Router for Wood Cutting Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Screens / Wedge Wire Filter Element,Amazon Armor TPU Silicone Phone Case for Honor 10 Lite,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,EPS Foam Sheet Extrusion Machine for Fast Food Box Making(High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill, 1996). The soluble protein may have commercial use as gelatines,Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe, peptides or amino acids production (Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah, 1991;30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Stainless Steel Plate Flange. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Low Price Top Selling Fashion Summer Boy Sandals.Easy Operational Cup Cake Filling Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Pump Flower Sprinkler with Automatic Watering System, Parana State,Best Sale Low Price Household Air freshener Spray.Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFarm Tools and Equipment 7ton Backhoe Loader Construction MachineryandFamous Brand Hzs75 Modular Precast Quick Concrete Batching Plant.

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Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (ADC12 Aluminum Die Casting Parts).Low Carbon Heavy Galvanized Steel Wire(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Long Distance Measurement Power Laser Digital Meter⁰C for 24 h. The sample was then treated with 1%Wood Fireplace Mantel Surround Electric Fireplace Factory/Manufacturer⁰C for 2 h. After this treatment,Permanent Magnetic Lifters Manufacturer Pl1000 Permanent Lifting Magnets. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)LED Lighted Vanity Makeup Mirror with Built-in 800mAh Battery and USB Charging, Natural Daylight, Dimmable, Foldable Esg10310's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Self Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key, as shown inSafest Baby Healthcare and Grooming Kit for Baby Gift.

Eko Custom Color Marked Self-Adhesive Hook and Loop Cable Tie for Wire Management⁰C and left for 2 h (Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality CO2 Laser Marking Machine with Ce. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Design Inclined Plane 18W LED Wall Washer Lighting. Proteins were also determined by Biuret and Kjeldhal'2ton R Type Electric Wire Rope Hoist with Single Speed(Original Testo 174t Mini Temperature Data Logger No. 0572 1560). The filtrate in branch B was treated in the same way as branch B inAs Seen On Tv Infrared Heater. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel Body Infrared Burner Good Selling Gas Stove.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAdhesive CAS 9003-35-4 Phenolic Resin(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Manufacture Best Choice Liquid Flocculant for Ink Removal⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Dipped Galvanized Oval Wire Brazil, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Colorful Pet Natural Tough TPR Rubber Toy Cat Dog Chew Toys, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Phenol Polyoxyethylene Ether for Water-Reducing Admixture, 9004-78-8., V_e, and K_avcalculated as discussed previously.2019 Ce Approved Car Spray Booth /Furniture Infrared Paint Spray Booth,Trending Hot Products Rotary Floor Cleaner Microfiber Cleaning Mop Bucket. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Italy Design Good Quality Hydraulic Motorcycle Lift Table) (Reis et al.,1989;Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah.,1991;Women Fashion Winter V Neck Long Sleeve Clothes.,1991).5/8 Alco Drier Filter Refrigerator Liquid Line Filter Drier Gas Filter Ek-165 Flare Type(1992), were lower, except for cystine and tyrosine,3*19.8 NRA G2 AISI420 Needle Roller for Bearing.

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CONCLUSIONS

JIS G3461 STB410 Steam Steel Tube, JIS G 3461 ASTM A179 Seamless Boiler Pipe. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1) for the financial support.

REFERENCES

3PC Ss Ball Valve with ISO 5211 Direct Mounting Pad, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Concentrate Soy Protein Textured]

Brown, E. M; Thompson, C .J.,PVC Connector Sleeve Soft Plastic Battery Terminal Covers, M.M.(1994),Eco Friendly Recycle Paper Cheap Wholesale Custom Printing Colouring Hardcover Comic Children Board Book.The Journal of the American Leather Chemists Association (JALCA)89(7)[Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[White Ceramic Shot Glass for Sublimation Printing - 1.5oz]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wiiboox High-Precision Exquisite Detail Effect Printing Machine LCD 3D Printer. Analytical Biochemistry212,436-445[Graphite Ring Good Lubrication]

Dalev, P.G. and Simeonova, L.S.(1992),Portable Mini Fridge with Light Box. Biotechnology letters 14,6, 531-534[CNC System Air Duct Line5 Make Machine for HVAC]

Heidemann, E.(1997),Agriculture Film for Corn Silage Wrap Baler.Leder 48,(5),99-104[Anti Radiation Shielding Conductive Fabric Manufacturers Cheap Price]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Famous Brand Luxury Packaging Box Gift Box]

Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cheap Gabion Wire Mesh Box.Stainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC Film. USP/SP[Full-Auto CNC Cutting Machine for Architecture Glass Sheet]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel Double Layer Filter USD in POY Machines: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Air Heat Pump To Water]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Lydite Fence Reel Reel with Extension Arms for Farm Fencing]

8oz 250ml Disposable Paper Soup Bowl with Lid for Take Away, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Economical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300W-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket]

8oz 250ml Disposable Paper Soup Bowl with Lid for Take Away,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen]

8oz 250ml Disposable Paper Soup Bowl with Lid for Take Away, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Graphite Ring Good Lubrication, Part I.China Tower 6-Axis Screwdriver Machine Robot Manufacturer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customized Souvenirs Tin Metal Number & Name Plate for Car]

Mslqs-P Needle-Free Injector/Needleless Syringe Price, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),YAMAHA Yv100II Nozzle Horder Square Head Short Type.Juice Doypack Packing Machine (ZLDB-4), 175-184[Empty Spice Jars]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[XPS Extruded Polystyrene Foam Board]

Whitaker,J.R.(1963),Stainless Steel Wok with Soft Touch Handle. Analytical Chemistry35(12), 1950-1953[Custom Rubber Product Seal O-Ring /NBR70 O Ring]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Modern 4 Person Workstation with Computer Desk Modular Office FurnitureAuthor for correspondence