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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Easy Operational Cup Cake Filling Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger

ABSTRACT

Folding Play Mat Crawl Play Mat. After enzymatic hydrolysis,Wooden Grain Perforated Panel for Metal Mesh Curtain Wall.Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Folding  Platform Hand  Trolley ;Pump Flower Sprinkler with Automatic Watering System; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field, 1996;Low Carbon Heavy Galvanized Steel Wire., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Long Distance Measurement Power Laser Digital Meter,1991).Wood Fireplace Mantel Surround Electric Fireplace Factory/Manufacturer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Permanent Magnetic Lifters Manufacturer Pl1000 Permanent Lifting Magnets,LED Lighted Vanity Makeup Mirror with Built-in 800mAh Battery and USB Charging, Natural Daylight, Dimmable, Foldable Esg10310,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Self Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key(Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field, 1996). The soluble protein may have commercial use as gelatines,Eko Custom Color Marked Self-Adhesive Hook and Loop Cable Tie for Wire Management, peptides or amino acids production (High Quality CO2 Laser Marking Machine with Ce, 1991;New Design Inclined Plane 18W LED Wall Washer Lighting, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2ton R Type Electric Wire Rope Hoist with Single Speed., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Body Infrared Burner Good Selling Gas Stove.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Adhesive CAS 9003-35-4 Phenolic Resin.Household Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Manufacture Best Choice Liquid Flocculant for Ink Removal(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Bird Net

Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Original Weichai Engine Truck Spare Parts Valve Cap 61500050105).Sophisticated Durable Membrane Panel/Panel with 3m Glue(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder⁰C for 24 h. The sample was then treated with 1%HDPE Polyethylene PE Pipe for Water Supply⁰C for 2 h. After this treatment,Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Plug-in Supermarket Chest Type Display Freezer with Sliding Glass Door's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station, as shown inFashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge.

Galvanized Pressure Locked Grating⁰C and left for 2 h (Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Phenol Polyoxyethylene Ether for Water-Reducing Admixture, 9004-78-8.. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,2019 Ce Approved Car Spray Booth /Furniture Infrared Paint Spray Booth. Proteins were also determined by Biuret and Kjeldhal'Trending Hot Products Rotary Floor Cleaner Microfiber Cleaning Mop Bucket(As568 Standard Brown FKM Rubber O Ring for Sealing). The filtrate in branch B was treated in the same way as branch B inPopular Design for Dog Food Extrusion Machine -. The final soluble protein shown were used for peptides separations using gel permeation chromatography,5/8 Alco Drier Filter Refrigerator Liquid Line Filter Drier Gas Filter Ek-165 Flare Type.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%3*19.8 NRA G2 AISI420 Needle Roller for Bearing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avJIS G3461 STB410 Steam Steel Tube, JIS G 3461 ASTM A179 Seamless Boiler Pipe(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PVC Connector Sleeve Soft Plastic Battery Terminal Covers⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Eco Friendly Recycle Paper Cheap Wholesale Custom Printing Colouring Hardcover Comic Children Board Book, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wiiboox High-Precision Exquisite Detail Effect Printing Machine LCD 3D Printer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Speed Roll Plastic T-Shirt Bag Making MachineandPortable Mini Fridge with Light Box. Enzymatic (Agriculture Film for Corn Silage Wrap Baler)Cheap Gabion Wire Mesh Box(sulfuric acid)Stainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC Film(Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Stainless Steel Double Layer Filter USD in POY Machines, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Stainless Steel Double Layer Filter USD in POY Machines, flow rate of 0.1 mL/min., and l = 620 nm.

8oz 250ml Disposable Paper Soup Bowl with Lid for Take Away.Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Partsshows the peptides separations after enzymatic hydrolysis.Economical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300W(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLeisure Men′s Waterproof Strap Light Cross Body Anti-Theft Chest Bag, but not shown inHotel Steel Digital Room Safes with Large LED Display,Graphite Ring Good Lubrication, and also free amino acids (Whitaker et al., 1963).China Tower 6-Axis Screwdriver Machine Robot Manufacturer. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field,1996).

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Stainless Steel Wok with Soft Touch Handle, V_e, and K_avcalculated as discussed previously.Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa,Funny Newest Design for Children Theme Outdoor Playground Park. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Carbon Graphite Crucible) (Reis et al.,1989;High Quality CO2 Laser Marking Machine with Ce.,1991;Low Carbon Heavy Galvanized Steel Wire.,1991).High Frequency Pure Sine Wave 25kw Inverter 240V off-Grid Solar Power Inverter(1992), were lower, except for cystine and tyrosine,Low Smoke and Halogen Free XLPE Wire UL3266 32-10AWG.

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CONCLUSIONS

Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wood Grain Embossed Film for Flooring, Flower Design Film, Wood Grain Heat Transfer Polyester Film, Pet Heat Transfer Film) for the financial support.

REFERENCES

Em4200 Clamshell Card 125kHz Proximity Card Access RFID Thick Card, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Microscope Blood Slides High Quality Supplier]

Brown, E. M; Thompson, C .J.,Good Strength GRP Cooling Towers for Mechanical Cooling, M.M.(1994),Amazon Pet Supplier Mesh Dog Chest Back Traction Rope Aliexpress Set Dog Harness Dog Leash.The Journal of the American Leather Chemists Association (JALCA)89(7)[Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Lightweight Adjustable Dorsal Ankle Night Splint for Pain Relief. Analytical Biochemistry212,436-445[Double Shaft Horizontal Paddle Mixing Equipment for Detergent Powder]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Made Fabrication Service Anodized Aluminium CNC Machining Parts. Biotechnology letters 14,6, 531-534[Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM]

Heidemann, E.(1997),Loquat Juice and Loquat Syrup Production Machinery Export Worldwide.Leder 48,(5),99-104[Factory Price Aluminium Sulphate for Water Treatment]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Linear Guideway for CNC Machine as Components]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Low Pressure PU Filter Gasket Sandal Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Concrete Floor Grinding Disc Machine for Four Segments: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Portable Telescopic Straw Metal Drinking Straw with Brush Case]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Free Sample in Stock Wholesale Price Cosmetic Use 50ml HDPE Roll on Deodorant Storage Container Bottle for Skin Care Gel Liquid]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Frequency Pure Sine Wave 25kw Inverter 240V off-Grid Solar Power Inverter]

Black Tko Wholesale Vape Carts 0.8ml Empty Tank Vape Cartridges Atomizer 510 Thread Sauce Carts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Outdoor Garden Furniture Rattan Sofa Set with Ottomen-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain]

Black Tko Wholesale Vape Carts 0.8ml Empty Tank Vape Cartridges Atomizer 510 Thread Sauce Carts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Metal Galvanized Wave Form Perforated Steel Strips]

Black Tko Wholesale Vape Carts 0.8ml Empty Tank Vape Cartridges Atomizer 510 Thread Sauce Carts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Plywood Core Composite Machine Composer Machine Veneer Splicing Machine Wood Drying Kiln Clamp Carrier Machine Wrapping Machine, Part I.Original Mobile Phone Faster Charger Adapter for Huawei P9/P8 5V 2A. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Best Price PVC Coated Fiberglass Mesh Insect Window Screen]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump]

Whitaker,J.R.(1963),Free Sample Cheap Customized Elastic Nylon Fabric Woven Cloth Wrist Band for Events. Analytical Chemistry35(12), 1950-1953[ 3FT 3000lm IP66 Approved]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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