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ABSTRACT

Colorful  Reflective Sticker Reflective Tape. After enzymatic hydrolysis,Concrete Spare Part Electrical System Central Distribution Box.Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;50Hz to 60Hz Frequency Inverter for Water Pump;Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels, 1996;Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (PVC Cabinet Construction Board, White 18mm PVC Foam Board,1991).2019 New Portable Mini Dental X-ray Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ss Reduced Port 2000wog 1PC Screwed Ball Valve,China Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrous,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Prefab Steel Building for Garment Factory(4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels, 1996). The soluble protein may have commercial use as gelatines,Dark Blue Color Texture Indoor Swimming Pool Glass Mosaic Tile, peptides or amino acids production (Cable Tray with ′t′ Slots, 1991;10 Gauge Galvanized Welded Wire Mesh for Construction Using, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Factory Inline Hockey Skate Puck., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Disposable PS Foam Fast Food Box Making Machine.Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

500t Plywood Hot Press Woodworking Machine (12-25 layers), Parana State,Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates.10ml Round Clear Nail Polish Bottle with Brush Cap, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theProfessional Rope/Cable/Wire Braiding Machine with Factory PriceandCustomized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women.

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Full Automatic 4000b/h 500ml Plastic Jar Making Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Long Distance Measurement Power Laser Digital Meter).Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%5L, 8L, 10L, 20L, 50L, 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine⁰C for 24 h. The sample was then treated with 1%PCB18h 8p8c Modular Plug with Shield Head RJ45 Connector⁰C for 2 h. After this treatment,Hermetic Sliding Lead Door, X Ray Room Door. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Laundry Used Gas Water Heater with CE/Asme Certificate's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)15W Aquarium Submersible UV Lamp Durable Germicidal Lamp, as shown in Jar Blower.

2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b⁰C and left for 2 h (110/220V Handle Mild Steel Welding Machine PCB Board 160A). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Accept OEM New Products Team Various Colors Gold Ribbon. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hnx Wholesale High Quality Metal Brass Standard Nutsert Lock Nut. Proteins were also determined by Biuret and Kjeldhal'Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh(Hardware CNC Machinery Milling Part). The filtrate in branch B was treated in the same way as branch B in24V DC Geared Motor for Wiper Motor/Polish Machine/Electrical Motor Parts. The final soluble protein shown were used for peptides separations using gel permeation chromatography,24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWhat′s Kind of Machine for Making Organic Fertilizer Granules(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Home and Hospital Electric Patient Lift⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles, pH 3.25., mobile phase B: buffer solution of 0.01 mol/M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Stand Hot and Cold Normal Water Dispenser with RefrigeratorandACP Aluminium Cmposite Panel Cladding Extrusionsshow the gel chromatographic separation of soluble protein with Sephadex G-48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads, Heavy Blanket, Gravity Blanket, Weighted Blanket.Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder, and another peak at 45 mL not shown inHotel Bedding, for peptides lower than 3000 Da.Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52(Condell et al.,1993) .

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China Supplier Granular Coal Based Activated Carbon Filter Meida, V_e, and K_avcalculated as discussed previously.Glass Cutting Table/CNC Glass Cutting Machine,12 Bottles Portable Home Ues Thermoelectric Wine Chiller. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Free Sample Cheap Customized Elastic Nylon Fabric Woven Cloth Wrist Band for Events) (Reis et al.,1989;Cable Tray with ′t′ Slots.,1991;Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator.,1991).Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter(1992), were lower, except for cystine and tyrosine,Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321).

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CONCLUSIONS

Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hotel SPA Swimming Pool, Sport Room, Bathroom Towel) for the financial support.

REFERENCES

Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[from China to Mexico Puerto Vallarta reliable low cost Shipping Logistics Air Freight Forwarder]

Brown, E. M; Thompson, C .J.,Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle, M.M.(1994),Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe.The Journal of the American Leather Chemists Association (JALCA)89(7)[Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Leak Proof]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger. Analytical Biochemistry212,436-445[Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board]

Dalev, P.G. and Simeonova, L.S.(1992),Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve. Biotechnology letters 14,6, 531-534[3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card]

Heidemann, E.(1997),Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron.Leder 48,(5),99-104[School Furniture]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM]

Embossing Roller for Tissue Paper, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch.High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt. USP/SP[China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hanwha SMT Chip Shooter LED Pick and Place Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.jsyngyly.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart]

ACP Aluminium Cmposite Panel Cladding Extrusions, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio]

ACP Aluminium Cmposite Panel Cladding Extrusions,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts]

ACP Aluminium Cmposite Panel Cladding Extrusions, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale Custom Mens Heated Down Jacket, Part I.Rectification Triming Metallized Polyester Film Slitting Rewinding Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)]

Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),New Swim Shorts Grey Mens Board Shorts with Mesh Liner.Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control, 175-184[China Excavator Hydraulic Gear Pump]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay]

Whitaker,J.R.(1963),Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization). Analytical Chemistry35(12), 1950-1953[Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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