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ABSTRACT

Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag. After enzymatic hydrolysis,VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A).ALS Stage 5W RGB Animation Laser Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Pet Anti-Static Needled Mat, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine, 1996;OEM Sheet Metal Fabrication for Aluminum Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hwf Tsp Core Bit for Geotechnical Drilling,1991).Price of Automatic Sludge Dewatering Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine,Worm Gear Operation Wafer Lined F3 Butterfly Valve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad(Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine, 1996). The soluble protein may have commercial use as gelatines,Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag, peptides or amino acids production (Positive Drive Turn Belt for Spiral Freezing Equipment, 1991;Chinese Herb Medicine Angelica Root Extract, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;S Series China Generator Helical-Worm Crushers Reduction Box., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Original Testo 174t Mini Temperature Data Logger No. 0572 1560. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water.Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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1060 1100 3003 3105 5052 5005 Aluminium Coils Manufacturer in China(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hairs Comb for Pet Dog Cats).Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom ABS&POM&PP&PC&Acrylic Plastic CNC Machining Turning Parts⁰C for 24 h. The sample was then treated with 1%CNC Machining Non-Standard Hardware Parts⁰C for 2 h. After this treatment,DOT Aluminum Beverage CO2 Gas Cylinder 5lb 10lb 15lb 20lb. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Galvanized Solar System Mounting Bracket Connector's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Digital Programmable Ntc Temperature Controller, as shown inDIN 7337 Aluminium Core Pulling Stainless Steel Rivet.

Water Meter Spare Parts Rubber Rings for Sealing⁰C and left for 2 h (Popular Lovely Animal Design Inflatable Slide for Kids). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stainless Steel HK Series Needle Roller Bearing HK0408. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Road Construction Fabric Non-Woven Geotextile 200GSM. Proteins were also determined by Biuret and Kjeldhal'High Polymer Modified Cement Based Elastic Waterproof Coating(Good Price 20kVA Single Phase Silent Diesel Generator). The filtrate in branch B was treated in the same way as branch B in12 Inch Light Bar. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFashion Shoulder Backpack Handbag School Bag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Sale Cute Food Plastic Pet Slow Feed Dog Bowl⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/FTTH Fiber Optic Fusion Splicing Toolkit, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Speed Cable Wire Winding Braiding Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Residential Reverse Osmosis 0.0001um Water Filter Water Filtration.Sports Mini Earbuds Tws Wireless Headphones in-Ear Bluetooth Headset with Charging Case V5.0shows the peptides separations after enzymatic hydrolysis.Rg178 Coaxial Cable with Ipex/Ipx Female to SMA Male Right Angle(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMushroom Head Stud Sert, but not shown inLED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light,China Cheap Polarized Sunglasses Hot Sale Metal Sunglasses, and also free amino acids (Whitaker et al., 1963).Folding Round Picnic Caravan Table RV Picnic Table Camping Table. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine,1996).

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Wood Veneer Peeling and Clipping Combined Machine, V_e, and K_avcalculated as discussed previously.Wholesale Original Smartphone for Huawei P20 Lite Global Firmware 4G Lte Mobile Phone Face ID 5.84 Screen Android 8.0 Front Camera 4GB 128GB ROM Cellphone,Wholesale High Quality Decoration Oil Painting, Home Decoration Painting, Art Painting (abstract sailing oil painting). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (ASTM Approved Popular Indoor Trampoline of Park) (Reis et al.,1989;Positive Drive Turn Belt for Spiral Freezing Equipment.,1991;OEM Sheet Metal Fabrication for Aluminum Parts.,1991).Si3n4 Full Ceramic  Bearing  608 Size 8X22X7(1992), were lower, except for cystine and tyrosine,32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping Mall.

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CONCLUSIONS

Kara Two-Way Dual 8′′ Line Array PRO Audio System Powerful PA Speaker. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CNC Machining Turning Anodized Aluminum Worm Gear) for the financial support.

REFERENCES

Corrugated Board Production Line, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality JAC Auto Parts Rear Wheel House]

Brown, E. M; Thompson, C .J.,Magnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtive, M.M.(1994),Car Air Conditional Compressor Magetic Clutch 5pk.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Automatic Food Metal Detector Machine for Production Line]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Engieered Veneer Faced Commericial Plywood. Analytical Biochemistry212,436-445[Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330)]

Dalev, P.G. and Simeonova, L.S.(1992),Hex Thin Head Nut. Biotechnology letters 14,6, 531-534[Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls]

Heidemann, E.(1997),Pharmaceutical Peptide Ghrp 2 Acetate CAS158861-67-7 Ghrp-2.Leder 48,(5),99-104[Coffee Bean Bags]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CAT6A FTP Patch Panel (WD6A-012)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Popular Water Bike / Hydro Bike / Aqua Bike (SK-8001)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[100W Dimmable LED Corn Bulb with Meanwell Driver]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[28113-1r100 Air Filter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil]

50 Grams Ozone Producing Device for Makeup Toner Producing Plant, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Flat Pack Temperary Container House 20FT 40FT-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pipe Thermostat with Sensor Ce Certificate]

50 Grams Ozone Producing Device for Makeup Toner Producing Plant,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Gold Star Square Glass Candle Holder for Wedding Decoration]

50 Grams Ozone Producing Device for Makeup Toner Producing Plant, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sweat Suit for Women Three Piece Tracksuit, Part I.Cold Rolled Stainless Steel Strip /Coils 410/430/409. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Solid Flex-Drain Pipe Length 40′]

FRP Water Tank GRP Fishing Tanks From Factory Supply, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Decorative House Stone Coated Metal Roof Tile.Integrated Crawler Rotary Underground Fully Hydraulic Tunnel Jumbo Drilling Rig, 175-184[Unimin Quartz Silica Sand 60-120 Mesh for Quartz Tube]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic]

Whitaker,J.R.(1963),6LTR Stainless Steel Foam Empty Fire Extinguisher Cylinder. Analytical Chemistry35(12), 1950-1953[Laparoscopy Clip Applicator for Abdominal Surgery Uses]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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