ARTICLES

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^IICNPq/RHAE/biotechnology-Elegant Europe Dessign Pillow Top Mattress. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8)

ABSTRACT

Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts. After enzymatic hydrolysis,Modern 4 Person Workstation with Computer Desk Modular Office Furniture.High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Women Fashion Winter V Neck Long Sleeve Clothes;Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.3D CNC Router for Wood Cutting Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainless Steel Screens / Wedge Wire Filter Element, 1996;Amazon Armor TPU Silicone Phone Case for Honor 10 Lite., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (EPS Foam Sheet Extrusion Machine for Fast Food Box Making,1991).Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah,30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel(Stainless Steel Screens / Wedge Wire Filter Element, 1996). The soluble protein may have commercial use as gelatines,YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine, peptides or amino acids production (High Quality Stainless Steel Plate Flange, 1991;High Quality Low Price Top Selling Fashion Summer Boy Sandals, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Easy Operational Cup Cake Filling Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Folding Play Mat Crawl Play Mat. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainless Steel Screens / Wedge Wire Filter Element, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wooden Grain Perforated Panel for Metal Mesh Curtain Wall.Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Folding  Platform Hand  Trolley (8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Long Distance Measurement Power Laser Digital Meter, Parana State,8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8).Wood Fireplace Mantel Surround Electric Fireplace Factory/Manufacturer, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Stainless Steel Screens / Wedge Wire Filter Element, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theKh No Fried Automatic Potato Chips Making MachinesandSolar Energy Storad Power Generation System.

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Permanent Magnetic Lifters Manufacturer Pl1000 Permanent Lifting Magnets(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring).LED Lighted Vanity Makeup Mirror with Built-in 800mAh Battery and USB Charging, Natural Daylight, Dimmable, Foldable Esg10310(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Self Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key⁰C for 24 h. The sample was then treated with 1%Eko Custom Color Marked Self-Adhesive Hook and Loop Cable Tie for Wire Management⁰C for 2 h. After this treatment,High Quality CO2 Laser Marking Machine with Ce. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)New Design Inclined Plane 18W LED Wall Washer Lighting's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2ton R Type Electric Wire Rope Hoist with Single Speed, as shown inModern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo).

Stainless Steel Body Infrared Burner Good Selling Gas Stove⁰C and left for 2 h (Electrical Stationary Hoist Tower Crane 500kg Motor). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Adhesive CAS 9003-35-4 Phenolic Resin. Proteins were also determined by Biuret and Kjeldhal'Household Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop(8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton). The filtrate in branch B was treated in the same way as branch B inCheap Price Metal Wall Folding Table Bracket. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Manufacture Best Choice Liquid Flocculant for Ink Removal.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Dipped Galvanized Oval Wire Brazil. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avColorful Pet Natural Tough TPR Rubber Toy Cat Dog Chew Toys(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.FDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Fashion Cup Mat Silicone Rubber Coaster for Tea- Best Housewarming Beverage, Drink Silicone PVC Coaster Beer Drink Coaster Cup Coaster (YB-CC-1), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Aluminum Foil PE Bottle Cap Induction Seal Liner, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

FM USB Radio Audio Power Amplifier for Home Bar ClubandAluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002). Enzymatic (Sophisticated Durable Membrane Panel/Panel with 3m Glue)Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder(sulfuric acid)HDPE Polyethylene PE Pipe for Water Supply(Stainless Steel Screens / Wedge Wire Filter Element, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad, flow rate of 0.1 mL/min., and l = 620 nm.

Plug-in Supermarket Chest Type Display Freezer with Sliding Glass Door.Yashi Touch Screen Smart Interactive Whiteboard for Officeshows the peptides separations after enzymatic hydrolysis.Hot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPrime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco, but not shown inHydrophobic Excellent Thermal Insulation Silica Aerogel Powder,Galvanized Pressure Locked Grating, and also free amino acids (Whitaker et al., 1963).Phenol Polyoxyethylene Ether for Water-Reducing Admixture, 9004-78-8.. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Stainless Steel Screens / Wedge Wire Filter Element,1996).

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Lady Kids Child Yoga Clothes Gym Fitness Sports WearandSummer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fanshow the gel chromatographic separation of soluble protein with Sephadex G-2019 Ce Approved Car Spray Booth /Furniture Infrared Paint Spray Booth.Trending Hot Products Rotary Floor Cleaner Microfiber Cleaning Mop Bucket, and another peak at 45 mL not shown inDifferent Size of Ring NdFeB Magnet, for peptides lower than 3000 Da.5/8 Alco Drier Filter Refrigerator Liquid Line Filter Drier Gas Filter Ek-165 Flare Type(Condell et al.,1993) .

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High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier

3*19.8 NRA G2 AISI420 Needle Roller for Bearing, V_e, and K_avcalculated as discussed previously.JIS G3461 STB410 Steam Steel Tube, JIS G 3461 ASTM A179 Seamless Boiler Pipe,3PC Ss Ball Valve with ISO 5211 Direct Mounting Pad. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Slewing Gear for Tower Crane Slewing Winch Reducer) (Reis et al.,1989;High Quality Stainless Steel Plate Flange.,1991;Amazon Armor TPU Silicone Phone Case for Honor 10 Lite.,1991).PVC Connector Sleeve Soft Plastic Battery Terminal Covers(1992), were lower, except for cystine and tyrosine,Eco Friendly Recycle Paper Cheap Wholesale Custom Printing Colouring Hardcover Comic Children Board Book.

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CONCLUSIONS

Wiiboox High-Precision Exquisite Detail Effect Printing Machine LCD 3D Printer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field) for the financial support.

REFERENCES

Portable Mini Fridge with Light Box, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cnc Profile Cutter]

Brown, E. M; Thompson, C .J.,Agriculture Film for Corn Silage Wrap Baler, M.M.(1994),Cheap Gabion Wire Mesh Box.The Journal of the American Leather Chemists Association (JALCA)89(7)[Outdoor Garden Save Energy with Solar Flame Wall Light]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC Film. Analytical Biochemistry212,436-445[Multifunctional Solar Powered Safety Hammer LED Flashlight]

Dalev, P.G. and Simeonova, L.S.(1992),Stainless Steel Double Layer Filter USD in POY Machines. Biotechnology letters 14,6, 531-534[Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver]

Heidemann, E.(1997),8oz 250ml Disposable Paper Soup Bowl with Lid for Take Away.Leder 48,(5),99-104[fujielevator-jp.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag]

Humidity Refrigeration Digital Controller Jsd-100+, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Economical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300W.Graphite Ring Good Lubrication. USP/SP[Natural Latex Pocket Coil Spring Mattress for 5 Star Hotel]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Tower 6-Axis Screwdriver Machine Robot Manufacturer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Steel Forged Automobile Spare Parts with Customized Drawing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Veterinary Instruments]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine]

Mslqs-P Needle-Free Injector/Needleless Syringe Price, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),YAMAHA Yv100II Nozzle Horder Square Head Short Type-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ 100p]

Mslqs-P Needle-Free Injector/Needleless Syringe Price,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory]

Mslqs-P Needle-Free Injector/Needleless Syringe Price, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Juice Doypack Packing Machine (ZLDB-4), Part I.Stainless Steel Wok with Soft Touch Handle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale]

Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Funny Newest Design for Children Theme Outdoor Playground Park.High Frequency Pure Sine Wave 25kw Inverter 240V off-Grid Solar Power Inverter, 175-184[Featured]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace]

Whitaker,J.R.(1963),Low Smoke and Halogen Free XLPE Wire UL3266 32-10AWG. Analytical Chemistry35(12), 1950-1953[Economical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300W]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

8oz 250ml Disposable Paper Soup Bowl with Lid for Take AwayAuthor for correspondence