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ABSTRACT

Galvanized Roller Shutter Window Torsion Springs. After enzymatic hydrolysis,100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale.Carbon Graphite Crucible. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Di DIN3352 Double Orifice Automatic Air Release Valve Dn65, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Magic Hair Twist   Curl Sponge Brush, 1996;Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Geyi Disposable Laparoscopic Visible Optical Trocar,1991).Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Bimetallic Thermometer Industrial Temperature Gauge,19 Network Cabinet with Lockable Rear Door,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery(Magic Hair Twist   Curl Sponge Brush, 1996). The soluble protein may have commercial use as gelatines,175mm Diamond Cutting Blade for Granite&Tiles, peptides or amino acids production (Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables, 1991;PU-Flex Duct Hose for Transporting Dust, Granules and Shavings, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Uprigth Touch-Screen Red Wine Chiller.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Magic Hair Twist   Curl Sponge Brush, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder.Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Efficiency Non-Expensive Cracking Agent, Parana State,Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly.Fiber Optic Sc APC Fast Connector for FTTH Drop Cable, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Magic Hair Twist   Curl Sponge Brush, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMolasses Fuel Ethanol Distillation PlantandCentral Loose Tube No Armored 2 - 24 Core Fiber Optic Cable.

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Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW)).Custom Striped Compression Soccer Socks for Adults(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738)⁰C for 24 h. The sample was then treated with 1%Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen⁰C for 2 h. After this treatment,High Tensile Strength Galvanized Steel Wire. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)24 Volt Fire Alarm Strobe Light Horn Strobe Sounder's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder, as shown inColumn Type Pave Rubber Tile Making Machine-Vulcanizing Press Machine.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pink French Loop Terry Knit Fabric for Jacket. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Mold Injection Molding Plastic Products White PC HousingandBuiltin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall. Enzymatic (Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit)Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262)(sulfuric acid)Cute Pink Cashmere Soft Plush Lining Warm Bootie(Magic Hair Twist   Curl Sponge Brush, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%3kw 24V 310mm Vertical Agv Drive Wheel Assembly, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%3kw 24V 310mm Vertical Agv Drive Wheel Assembly, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Classic Black Color Commercial Glass Door Multi Deck Refrigerator. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires) for the financial support.

REFERENCES

Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Canton Fair Export Supply Black General Edition 40 Inch LCD TV]

Brown, E. M; Thompson, C .J.,12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026), M.M.(1994),Pneumatic Control Shuttle Valve NPT Thread.The Journal of the American Leather Chemists Association (JALCA)89(7)[Electrical Stationary Hoist Tower Crane 500kg Motor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing. Analytical Biochemistry212,436-445[FM USB Radio Audio Power Amplifier for Home Bar Club]

Dalev, P.G. and Simeonova, L.S.(1992),OEM 15PCS Electric Face Makeup Brush with Ce Certificate. Biotechnology letters 14,6, 531-534[Yashi Touch Screen Smart Interactive Whiteboard for Office]

Heidemann, E.(1997),New Styles Fashion Erotic Women Underwear Sexy Lingerie.Leder 48,(5),99-104[150W Solar IP Camera with LED Street Light]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco]

530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale.Stand Hot and Cold Normal Water Dispenser with Refrigerator. USP/SP[Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Construction Welding Rods Electrode E6013 E7018 E7016: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Lady Kids Child Yoga Clothes Gym Fitness Sports Wear]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Air Cooling Cnc Spindle Motor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan]

High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Different Size of Ring NdFeB Magnet]

High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier]

High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Lydite Fence Reel Reel with Extension Arms for Farm Fencing, Part I.1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Slewing Gear for Tower Crane Slewing Winch Reducer]

Customized 3D Medal for Religious Medal Gift, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules.Dbry-320 Christmas Stickers Labels Printing Machine, 175-184[Belt Tensioner Cummins Nt855-Ga 3655291]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer]

Whitaker,J.R.(1963),Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor. Analytical Chemistry35(12), 1950-1953[Mechanical Seal 101 Type for Pump and Auto]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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