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ABSTRACT

Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger. After enzymatic hydrolysis,Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve.Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hanwha SMT Chip Shooter LED Pick and Place Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (ACP Aluminium Cmposite Panel Cladding Extrusions, 1996;New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Custom Mens Heated Down Jacket,1991).Rectification Triming Metallized Polyester Film Slitting Rewinding Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker,New Swim Shorts Grey Mens Board Shorts with Mesh Liner,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control(ACP Aluminium Cmposite Panel Cladding Extrusions, 1996). The soluble protein may have commercial use as gelatines,Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization), peptides or amino acids production (Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil, 1991;Marine Mooring Hardware, Anchor Chain, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-QJ-M Moving small manual brick making machine with low cost. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (ACP Aluminium Cmposite Panel Cladding Extrusions, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device.Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Braiding Machine for Weaving Flexible Metal Hose(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Polypropylene Woven Bag for Sludge Dewatering(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts).China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing⁰C for 24 h. The sample was then treated with 1%High Quality Industrial Grade Anhydrous Sodium Sulfate⁰C for 2 h. After this treatment,Professional Factory Supply Design Cat Tree Pet Product. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Peb Steel Structure Building Materials H-Section I Beam Steel, as shown inSeamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing.

Aluminum Oxide Non Woven Polishing Wheel for Machine⁰C and left for 2 h (Plywood Core Composite Machine Composer Machine Veneer Splicing Machine Wood Drying Kiln Clamp Carrier Machine Wrapping Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.CAT6A FTP Patch Panel (WD6A-012). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine. Proteins were also determined by Biuret and Kjeldhal'Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant(Flour Making Machine Wheat Maize Flour Mill Machinery). The filtrate in branch B was treated in the same way as branch B inRFID Thick Clamshell Card 125kHz Tk4100/Em4200 Read Only Proximity Access Card. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Famous Brand Luxury Packaging Box Gift Box.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Qualified Medical Grade Thin Wall Stainless Steel Tube. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Structural Synthetic Fiber for Concrete⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Efficiency Fine Powder Vibrating Screener, V_e, and K_avcalculated as discussed previously.High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management,Plant Stands & Accessories Glass Hanging Flower Pot Bracket. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kits) (Reis et al.,1989;Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil.,1991;New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine.,1991).Wooden Frame Stretched Canvas, Painting Canvas(1992), were lower, except for cystine and tyrosine,Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine.

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CONCLUSIONS

SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616)) for the financial support.

REFERENCES

UF Membrane Aqu160 with Competitive Price and High Quality, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Dental Sterilization Tube Convertor Saliva Ejector Suction Adaptor]

Brown, E. M; Thompson, C .J.,Atf-805 Auto Car Transmission System Flushing Machine, M.M.(1994),43 Glass Beads Cat Eye Aluminum Road Stud.The Journal of the American Leather Chemists Association (JALCA)89(7)[Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041). Analytical Biochemistry212,436-445[CNC Machining Service High Precision CNC Mechanical Parts]

Dalev, P.G. and Simeonova, L.S.(1992),High Precision C45 Steel CNC Lathe Parts. Biotechnology letters 14,6, 531-534[500t Plywood Hot Press Woodworking Machine (12-25 layers)]

Heidemann, E.(1997),I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max.Leder 48,(5),99-104[Clean SMT 110g Microfiber Wiper Roller]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks]

Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate.Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1. USP/SP[Precision Customized OEM Auto Hardware Lathe CNC Machining Parts]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Blue Steel Packing Strip]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Food Grade PTFE Coated Fiberglass Mesh Baking Sheet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Glue Packing Tape]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[10A MPPT Solar Controllers PV 60V for off-Grid]

Beverage Cooling Cube Ice Making/Ice Maker Machinery, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[FTTH Fiber Optic Fusion Splicing Toolkit]

Beverage Cooling Cube Ice Making/Ice Maker Machinery,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Safe Durable Scaffolding Trapdoor Plank for Construction]

Beverage Cooling Cube Ice Making/Ice Maker Machinery, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Transparent Composite Fiberglass Roof Tiles, Part I.Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Supplied High Strength Marine Anchor Chain]

Two Layer License Plates with Wide Border Embossed, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Electrical Barber Shop Equipment Electric Hair Curling Wand Iron.Really Microfiber PVC Leather for Sofa Furniture, 175-184[www.hzd-industry.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch]

Whitaker,J.R.(1963),94V0 Circuit Board Mobile Phone PCB Motherboard. Analytical Chemistry35(12), 1950-1953[Small Cleaning Brush ESD Anti Static Brush ESD Brush Black]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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