ARTICLES

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^IICNPq/RHAE/biotechnology-Graphic 128X128 Dots Cog LCD. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen

ABSTRACT

Tcn Sex Toy Vending Machines for Sale S800-10. After enzymatic hydrolysis,Silver 8 Inch Double Thick Round Cake Board.High Precision Machining Auto Parts Metal Contact. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Paper Sticky Lint Remover Roller/Pet Hair Roller, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Safe Durable Scaffolding Trapdoor Plank for Construction, 1996;China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Various Sublimation Blank Home Supply Fabric Bath Towel etc,1991).High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments,Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Best Quality Bending Metal Mould with All Kinds Metal Materials(Safe Durable Scaffolding Trapdoor Plank for Construction, 1996). The soluble protein may have commercial use as gelatines,New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus, peptides or amino acids production (Milling Precision Stainless Steel CNC Machinery Parts, 1991;U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Good Price Stand-up Zipper Doy Pouch Filling Packing Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Safe Durable Scaffolding Trapdoor Plank for Construction, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1).Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Food Machine Cube Ice Machine for Commercial Use(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High-End Business Magnet Leather Ring Binder Notebook(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor).Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace⁰C for 24 h. The sample was then treated with 1%Eyes- Caring LED Night Light for Nursing Reading with Soft Light⁰C for 2 h. After this treatment,China Bearing with Housing Bearing / Pillow Block Bearing. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)The Most Competitive Sodium Gluconate for Water Reducer, as shown inBest Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor.

Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles⁰C and left for 2 h (Must 4kw 2HP Pump Power Inverter Ags Bts Available). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Electric Magic Hot Hair Styling Curling Iron with Brushes. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Melt Pressure Transducer (PT133). Proteins were also determined by Biuret and Kjeldhal'Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe(Accept OEM New Products Team Various Colors Gold Ribbon). The filtrate in branch B was treated in the same way as branch B inDisposable Temperature Probe. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEducational Toys, Kid Eduactional Toys House, Children Educational Toys Game(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Durable Aluminum Flag Pole with Dome Top Tangle Free Finial, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mint Flavor 50ml Home Foam Toothpaste) for the financial support.

REFERENCES

Reusable Metal Stainless Steel Drinking Straws, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Blue Light Desk Lamp]

Brown, E. M; Thompson, C .J.,Designed Steel Frame Factory Building, M.M.(1994),Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate.The Journal of the American Leather Chemists Association (JALCA)89(7)[Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table. Analytical Biochemistry212,436-445[EPS Foam Sheet Extrusion Machine for Fast Food Box Making]

Dalev, P.G. and Simeonova, L.S.(1992),Scouring Pads Making Machine for Brush King. Biotechnology letters 14,6, 531-534[79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar]

Heidemann, E.(1997),Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor.Leder 48,(5),99-104[Li Polymer Power Bank]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Layer Egg Chicken Cage/Poultry Farm House Design Chicken Poultry Shed]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars]

Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Used/Second Hand 120mpm Continous Galvanizing Line/Cgi-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Round Shape Aluminum PCB and PCBA]

Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3HP Industrial Cooling System Water Cooled Chiller]

Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie, Part I.Racing Preformance PTFE Teflon Alloy Fuel Adapter. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[355nm UV Laser Marking Equipment for Crystal Materials]

Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Stainless Steel Expanded Metal Mesh.Popular Lovely Animal Design Inflatable Slide for Kids, 175-184[High Quality Neodymium Loudspeaker Magnet]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads]

Whitaker,J.R.(1963),Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware. Analytical Chemistry35(12), 1950-1953[LED Emergency Exit Lights]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Plastic Caps Making Machine / Injection Molding MachineAuthor for correspondence