ARTICLES

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ABSTRACT

Air Bubble Insulation Thermal Building Insulation Material. After enzymatic hydrolysis,LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh.Rechargeable Battery Operated Cooling Folding Electric Fan. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve;21.5′′ Wall Mount Display Capacitive Touch Monitor; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Pagoda Gazebo Tent in Guangzhou for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe, 1996;Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Black Small Fridge for Showcase,1991).Microwave Motion Sensor Module Doppler Radar Detector (HW-M10). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile,Zircon Butterfly Open Ring Diamond Fashion Woemn Rings,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation(Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe, 1996). The soluble protein may have commercial use as gelatines,Spare Parts Custom Injection Plastic Moulding Manufacturer, peptides or amino acids production (Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill, 1991;96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Kingdomway Brand Vitamin a 1000., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Speed Roll Plastic T-Shirt Bag Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine.Wholesale Black Galvanized Head Stainless Steel Screw-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Nylon Weatherproof PA6 Strain Relief Cable Gland, Parana State,Freshwater Pearl Butterfly Flower Bride Hairpin.Steel Roller Embossed Paper 2019 New Design, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theElegant Europe Dessign Pillow Top Mattressand2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable.

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Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Factory Inline Hockey Skate Puck).Factory Price DIN5685 Galvanized Marine Anchor Link Chain(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001)⁰C for 24 h. The sample was then treated with 1%6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck⁰C for 2 h. After this treatment,High Level Application Waterproof Plastic Box. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse, as shown inBest Price Custom Embroidery Logo Hotel Slippers.

Water Slide for Indoor Play Structure⁰C and left for 2 h (New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,1220*2440mm Concrete Formwork black film faced plywood. Proteins were also determined by Biuret and Kjeldhal'Washami Teeth Care for Whitening Cool Mint Toothpaste(Automatic Food Metal Detector Machine for Production Line). The filtrate in branch B was treated in the same way as branch B inSteel Casting. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSHXJ1500 CNC Diamond Wire Stone Cutting Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2019 Foam Concrete Machine/Foamed Concrete Block Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Yyh Series One Dimension Tumbling Mixer Mixing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330)andODU 14 Pin Industrial Circular Connector. Enzymatic (Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board)Classical New Coming Plastic Female Sunglasses(sulfuric acid)OEM Double Sided Rigid PCB Circuit Board Manufacturer(Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Robot of World Robotics Competition, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Robot of World Robotics Competition, flow rate of 0.1 mL/min., and l = 620 nm.

Professional Rope/Cable/Wire Braiding Machine with Factory Price.Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girlsshows the peptides separations after enzymatic hydrolysis.Aluminum PCB Circuit Board SMD PCBA LED PCB(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCAT6A FTP Patch Panel (WD6A-012), but not shown inLED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine,Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159), and also free amino acids (Whitaker et al., 1963).Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe,1996).

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CONCLUSIONS

Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Vinyl Glove in Work & Labor Glove) for the financial support.

REFERENCES

High Quality Aluminum Heat Sink for Electronics Computer Electric, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Gas Pressure Reducing Flowmeter]

Brown, E. M; Thompson, C .J.,5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover, M.M.(1994),Surprise! Range Hood Free! ! ! Juice Cart.The Journal of the American Leather Chemists Association (JALCA)89(7)[Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Lifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Steam Eye Mask Heating One Eye Mask for Sleeping. Analytical Biochemistry212,436-445[Good Strength GRP Cooling Towers for Mechanical Cooling]

Dalev, P.G. and Simeonova, L.S.(1992),Automatic French Fries Frying Machine with Low Price. Biotechnology letters 14,6, 531-534[Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book]

Heidemann, E.(1997),Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns.Leder 48,(5),99-104[XL-21 Outdoor Waterproof Seal Metal Power Distribution Cabinet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps]

Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software.Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security. USP/SP[Dxz Limit Switch Tower Crane Spare Parts]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic Chewing Dog Food Snack Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Orange Monofilament Fishing Tackle Nylon Fish Net]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tamping Rammer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage]

Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Good Quality Pillow for Pregnant Wome Body Pregnant Pillow Case]

Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3)]

Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:T90 High Power Latching Relay Mini Power Relay, Part I.Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing]

Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Polyglycolic Acid Absorable Suture.No. 1062 20PCS Tools, 175-184[Residential Window Louvers]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[OEM 15PCS Electric Face Makeup Brush with Ce Certificate]

Whitaker,J.R.(1963),Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant. Analytical Chemistry35(12), 1950-1953[Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel PipeAuthor for correspondence