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ABSTRACT

Small Cleaning Brush ESD Anti Static Brush ESD Brush Black. After enzymatic hydrolysis,PAC /Poly Aluminium Chloride for Water Treatment.Aluminum Sheet Shearing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Electrical PV DC Solar Cable;Tomato Bubble and High Pressure Washing Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Upholstery Ribbon Christmas Tape, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate, 1996;2+1 Flavors Soft Ice Cream Machine with Pre-Cooling., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver,1991).40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Best Sale Low Price Household Air freshener Spray,Factory Supplied High Strength Marine Anchor Chain,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Humidity Refrigeration Digital Controller Jsd-100+(Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate, 1996). The soluble protein may have commercial use as gelatines,Elegant Europe Dessign Pillow Top Mattress, peptides or amino acids production (8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8), 1991;Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Modern 4 Person Workstation with Computer Desk Modular Office Furniture., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Women Fashion Winter V Neck Long Sleeve Clothes. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line.3D CNC Router for Wood Cutting Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel Screens / Wedge Wire Filter Element(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Liquid Spouted Stand up Pouch with Custom Logo).High Quality Stainless Steel Plate Flange(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Low Price Top Selling Fashion Summer Boy Sandals⁰C for 24 h. The sample was then treated with 1%Easy Operational Cup Cake Filling Machine⁰C for 2 h. After this treatment,Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Folding Play Mat Crawl Play Mat's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wooden Grain Perforated Panel for Metal Mesh Curtain Wall, as shown inFactory Wholesale Zinc Plated Carbon Steel Hex Nut.

Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1⁰C and left for 2 h (OEM Double Sided Rigid PCB Circuit Board Manufacturer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Folding  Platform Hand  Trolley . A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pump Flower Sprinkler with Automatic Watering System. Proteins were also determined by Biuret and Kjeldhal'Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant(Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan). The filtrate in branch B was treated in the same way as branch B inSoft Baby Suites Dinosaure Pijamas Kigurumi. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Low Carbon Heavy Galvanized Steel Wire. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLong Distance Measurement Power Laser Digital Meter(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Permanent Magnetic Lifters Manufacturer Pl1000 Permanent Lifting Magnets⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/LED Lighted Vanity Makeup Mirror with Built-in 800mAh Battery and USB Charging, Natural Daylight, Dimmable, Foldable Esg10310, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Self Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe) for the financial support.

REFERENCES

HDPE Polyethylene PE Pipe for Water Supply, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Carving Stone Wash Sink]

Brown, E. M; Thompson, C .J.,Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad, M.M.(1994),Plug-in Supermarket Chest Type Display Freezer with Sliding Glass Door.The Journal of the American Leather Chemists Association (JALCA)89(7)[Mouse Pad with Gel Wrist Rest]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[European Good Quality Bathroom Simple Sliding Glass Shower Cabina]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station. Analytical Biochemistry212,436-445[Quality Horizontal Flat Bed Metal CNC Turning Lathe Machine Price Ck6163]

Dalev, P.G. and Simeonova, L.S.(1992),Galvanized Pressure Locked Grating. Biotechnology letters 14,6, 531-534[2019 Foam Concrete Machine/Foamed Concrete Block Making Machine]

Heidemann, E.(1997),Phenol Polyoxyethylene Ether for Water-Reducing Admixture, 9004-78-8..Leder 48,(5),99-104[Stainless Steel Lag Screw Factory & Manufacturer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Absorbable Surgical Sutures Plain Catgut Thread USP1#]

Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),2019 Ce Approved Car Spray Booth /Furniture Infrared Paint Spray Booth.Trending Hot Products Rotary Floor Cleaner Microfiber Cleaning Mop Bucket. USP/SP[Silver 8 Inch Double Thick Round Cake Board]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-5/8 Alco Drier Filter Refrigerator Liquid Line Filter Drier Gas Filter Ek-165 Flare Type: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ODU 14 Pin Industrial Circular Connector]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[1325 Unloading System 3D Engraving CNC Router]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine]

3*19.8 NRA G2 AISI420 Needle Roller for Bearing, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),JIS G3461 STB410 Steam Steel Tube, JIS G 3461 ASTM A179 Seamless Boiler Pipe-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve]

3*19.8 NRA G2 AISI420 Needle Roller for Bearing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Afen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price]

3*19.8 NRA G2 AISI420 Needle Roller for Bearing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:3PC Ss Ball Valve with ISO 5211 Direct Mounting Pad, Part I.PVC Connector Sleeve Soft Plastic Battery Terminal Covers. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Outdoor Garden Furniture Rattan Sofa Set with Ottomen]

Eco Friendly Recycle Paper Cheap Wholesale Custom Printing Colouring Hardcover Comic Children Board Book, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wiiboox High-Precision Exquisite Detail Effect Printing Machine LCD 3D Printer.Portable Mini Fridge with Light Box, 175-184[www.zgnfsjx.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Bimetallic Thermometer Industrial Temperature Gauge]

Whitaker,J.R.(1963),Agriculture Film for Corn Silage Wrap Baler. Analytical Chemistry35(12), 1950-1953[UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Cake/Cookies/Bread Automatic Package and Packing MachineAuthor for correspondence