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ABSTRACT

No Lead Bronze Flange Manufacturer. After enzymatic hydrolysis,Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube.Snsc Fd30 Side Shift New Diesel Forklift 3ton. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117);Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Laptop Bag College Men Backpack Leather Back Pack Bags, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pet Dog Grooming Rake Dematting Tool Shedding Comb, 1996;Custom Apparel Applique Round Patch School Club Embroidery Collar Patch., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery,1991).CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror,Automatic Food Metal Detector Machine for Production Line,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Multi-Functional Backpack Manual 52cc Brush Cutter(Pet Dog Grooming Rake Dematting Tool Shedding Comb, 1996). The soluble protein may have commercial use as gelatines,Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa, peptides or amino acids production (Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine, 1991;Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;White Cotton Satin Hotel Duvet Cover (DPFB8055)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Small Cleaning Brush ESD Anti Static Brush ESD Brush Black.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PAC /Poly Aluminium Chloride for Water Treatment. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pet Dog Grooming Rake Dematting Tool Shedding Comb, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aluminum Sheet Shearing Machine.Electrical PV DC Solar Cable-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Tomato Bubble and High Pressure Washing Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse, Parana State,Gld Brand Padlock Brass Popular in 5 Continents  .Best Sale Low Price Household Air freshener Spray, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Pet Dog Grooming Rake Dematting Tool Shedding Comb, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the Miniature Ball Bearing (616)andHot Sell Mechanical Bar Screen for Water Treatment.

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Factory Supplied High Strength Marine Anchor Chain(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone).Humidity Refrigeration Digital Controller Jsd-100+(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Elegant Europe Dessign Pillow Top Mattress⁰C for 24 h. The sample was then treated with 1%8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8)⁰C for 2 h. After this treatment,Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Modern 4 Person Workstation with Computer Desk Modular Office Furniture's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill, as shown inWorm Gear Operation Wafer Lined F3 Butterfly Valve.

Women Fashion Winter V Neck Long Sleeve Clothes⁰C and left for 2 h (China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,3D CNC Router for Wood Cutting Machine. Proteins were also determined by Biuret and Kjeldhal'Stainless Steel Screens / Wedge Wire Filter Element( Jar Opener). The filtrate in branch B was treated in the same way as branch B inZirconium Abrasive Flber Disc for Heat Resistant Steel. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Amazon Armor TPU Silicone Phone Case for Honor 10 Lite.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%EPS Foam Sheet Extrusion Machine for Fast Food Box Making. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLuxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel, pH 3.25., mobile phase B: buffer solution of 0.01 mol/YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Wooden Grain Perforated Panel for Metal Mesh Curtain Wall.4 Axis CNC Woodworking Machinery Engraver Routershows the peptides separations after enzymatic hydrolysis.Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inEnergy Saving Track Rail Dimmable 15W 20W 30W LED Track Light, but not shown inPacking 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck,Folding  Platform Hand  Trolley , and also free amino acids (Whitaker et al., 1963).Pump Flower Sprinkler with Automatic Watering System. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Pet Dog Grooming Rake Dematting Tool Shedding Comb,1996).

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Long Distance Measurement Power Laser Digital Meter, V_e, and K_avcalculated as discussed previously.Wood Fireplace Mantel Surround Electric Fireplace Factory/Manufacturer,Permanent Magnetic Lifters Manufacturer Pl1000 Permanent Lifting Magnets. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security) (Reis et al.,1989;Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine.,1991;Custom Apparel Applique Round Patch School Club Embroidery Collar Patch.,1991).LED Lighted Vanity Makeup Mirror with Built-in 800mAh Battery and USB Charging, Natural Daylight, Dimmable, Foldable Esg10310(1992), were lower, except for cystine and tyrosine,Self Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key.

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CONCLUSIONS

Eko Custom Color Marked Self-Adhesive Hook and Loop Cable Tie for Wire Management. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (2+1 Flavors Soft Ice Cream Machine with Pre-Cooling) for the financial support.

REFERENCES

High Quality CO2 Laser Marking Machine with Ce, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[140mm Professional Test Pencil Screwdriver Voltage Tester Pen (FXT-09)]

Brown, E. M; Thompson, C .J.,New Design Inclined Plane 18W LED Wall Washer Lighting, M.M.(1994),2ton R Type Electric Wire Rope Hoist with Single Speed.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Anchor Chain]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hotel SPA Swimming Pool]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Body Infrared Burner Good Selling Gas Stove. Analytical Biochemistry212,436-445[High Polymer Modified Cement Based Elastic Waterproof Coating]

Dalev, P.G. and Simeonova, L.S.(1992),High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier. Biotechnology letters 14,6, 531-534[Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter]

Heidemann, E.(1997),Adhesive CAS 9003-35-4 Phenolic Resin.Leder 48,(5),99-104[Laundry Used Horizontal Gas Water Heater with CE Certificate]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[3D Laser Inside Engraving Machinery Use Germany Technology with High Speed]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Picc Puncture Care Kit]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Dipped Galvanized Oval Wire Brazil: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Sale Safety Vinyl PVC Flooring]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cable Trunking Suppliers High Quality Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Tianjin Manufacturer PTFE Coated Butterfly Valve]

Colorful Pet Natural Tough TPR Rubber Toy Cat Dog Chew Toys, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wood Grain Embossed Film for Flooring, Flower Design Film, Wood Grain Heat Transfer Polyester Film, Pet Heat Transfer Film-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag]

Colorful Pet Natural Tough TPR Rubber Toy Cat Dog Chew Toys,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Good Price Stand-up Zipper Doy Pouch Filling Packing Machine]

Colorful Pet Natural Tough TPR Rubber Toy Cat Dog Chew Toys, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:FDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit, Part I.Custom Fashion Cup Mat Silicone Rubber Coaster for Tea- Best Housewarming Beverage, Drink Silicone PVC Coaster Beer Drink Coaster Cup Coaster (YB-CC-1). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road]

Aluminum Foil PE Bottle Cap Induction Seal Liner, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002).Sophisticated Durable Membrane Panel/Panel with 3m Glue, 175-184[Clear Acrylic Pet Accessories Bed Dog Bed with Feeder]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Biomass Drum Wood Chipper with Competitive Price]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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