ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine

ABSTRACT

60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light. After enzymatic hydrolysis,Solar Energy Storad Power Generation System.110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Custom Aluminum Cover Frame Half Aluminum Cover Profile;Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.DC24V 36W Solar Fan Motor PCBA Card, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Monocular Hunting Wargame Mini Scope Digital Night Vision, 1996;Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor,1991).Italy Design Good Quality Hydraulic Motorcycle Lift Table. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Menthol E Liquid E Juice for EGO E Cigarettes,Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9(New Monocular Hunting Wargame Mini Scope Digital Night Vision, 1996). The soluble protein may have commercial use as gelatines,Portable Telescopic Straw Metal Drinking Straw with Brush Case, peptides or amino acids production (Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware, 1991;Etching Rear Trunk Sill Plates for Hyundai Tucson, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Quality Horizontal Flat Bed Metal CNC Turning Lathe Machine Price Ck6163., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Lab Equipment Sr Paper Pulp Beating Freeness Test Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Monocular Hunting Wargame Mini Scope Digital Night Vision, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Car Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima.Popular Water Bike / Hydro Bike / Aqua Bike (SK-8001)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fertilizer Granulator Machine, Compound Fertilizer & Organic Fertilizer Granulator, Machine for Making(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Ladle Refining Melting Steel Furnace Casting Ladle, Parana State,Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine.Lishui Factory High Quality Metal Rolling Ball Screw, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (New Monocular Hunting Wargame Mini Scope Digital Night Vision, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGood 0.38mm Color F-Green PVB Interlayer Film for Architecture Laminated GlassandHome Machine 2019 New Commercial Treadmill Gym Equipment.

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Y-Spring Threaded Check Valve, 800 Wog(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern).OEM CNC Processing Precision Plating/Anodizing/Painting Black Machining Part(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Factory Custom Highly White Clear 750ml Tequila Liquor Glass Bottle⁰C for 24 h. The sample was then treated with 1%Galvanized Eye Grab Bend Hook⁰C for 2 h. After this treatment,Transparent LED Display P3.91 Glass Wall LED Screen. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Surgical Power Tool Medical Care Orthopedic Mini Bone Saw's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Micro Small Plastic Product Injection Molding Making Machine, as shown inHigh Pressure Braided Rubber Oil Hose.

Glass Lifting Machine with Sucker for Glass Movements⁰C and left for 2 h (25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Anodized Gold Brush Finish Aluminum Sheet Coil. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Popular Industrial Sewing Quilting Machine With CO. Proteins were also determined by Biuret and Kjeldhal'Chinese Manufacturer Provide First-Class Service for Non Stretch/Elastic Allover Lace(Hot Sale Cute Food Plastic Pet Slow Feed Dog Bowl). The filtrate in branch B was treated in the same way as branch B inMug Press Heating Element. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pure Sine Wave Power Frequency Inverter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCopy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/33kv Dry Type Resin Casted Distribution Transformer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor.High Tensile Strength Galvanized Steel Wireshows the peptides separations after enzymatic hydrolysis.LED Emergency Exit Lights(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMonochrome Graphic Industrial Control LCD Display Module 128X64, but not shown inCustomized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V,Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve, and also free amino acids (Whitaker et al., 1963).25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (New Monocular Hunting Wargame Mini Scope Digital Night Vision,1996).

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Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic, V_e, and K_avcalculated as discussed previously.Leisure Men′s Waterproof Strap Light Cross Body Anti-Theft Chest Bag,Wholesale Aluminum Touring Pre-Rig Lighting Truss with Wheels. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002)) (Reis et al.,1989;Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware.,1991;Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine.,1991).43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump(1992), were lower, except for cystine and tyrosine,Aluminum Adjustable Quick Assemble Stage with Steps.

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CONCLUSIONS

Hardware CNC Machinery Milling Part. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Sale Coral Fleece Hotel Bathrobe (DPFT8070)) for the financial support.

REFERENCES

Customized 8 Layers Round LED Panel Aluminum PCB for LED Light, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Decoration Capillary Stainless Steel Groove Tubes]

Brown, E. M; Thompson, C .J.,Basic Style Buffalo Leather Safety Shoes Ce Certificate S3 (SN1388), M.M.(1994),Gamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge.The Journal of the American Leather Chemists Association (JALCA)89(7)[Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3). Analytical Biochemistry212,436-445[Paper Sticky Lint Remover Roller/Pet Hair Roller]

Dalev, P.G. and Simeonova, L.S.(1992),Resistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape. Biotechnology letters 14,6, 531-534[Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen]

Heidemann, E.(1997),Linear Guideway for CNC Machine as Components.Leder 48,(5),99-104[Flexible PVC Magnetic Rubber Sheet with Roll]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets]

Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts.40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5). USP/SP[Colorful 100% Cotton Hotel Face Towels]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504r: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Best Selling Colorful Branded Custom Logo Polarized Sunglasses Women Men 2018 Fashion UV400 Cat3 Mirrored Sun Shade Glasses]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hydraulic System]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material]

Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Original Smartphone for Huawei P20 Lite Global Firmware 4G Lte Mobile Phone Face ID 5.84 Screen Android 8.0 Front Camera 4GB 128GB ROM Cellphone]

Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pneumatic Control Shuttle Valve NPT Thread]

Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:External Retaining Ring for Shaft, DIN471, D1400, Part I.Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Fashion PVC Sequins Adjustable Women Brace Suspenders]

Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Rotary Vibrating Screen/Sieve/Sifter/Griddle Machine.PTZ Camera Two-Way  Audio  Baby Monitor Simple Easy to Operate, 175-184[China Acupressure Mat With Magnets Pyramids]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Synergist for Soap; Water Softener]

Whitaker,J.R.(1963),Custom Rubber Product Seal O-Ring /NBR70 O Ring. Analytical Chemistry35(12), 1950-1953[Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Eye Steam MaskAuthor for correspondence