ARTICLES

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ABSTRACT

Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping. After enzymatic hydrolysis,Ferrule Woven Rope Wire Mesh /SS316 Decorative Rope Mesh.Integrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Metal Material and Sports Theme Custom Metal Medal;Portable Installation and Ce Certification 20g/Hr OEM Air Purifier; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Full Computer Plain Socks Knitting Machine with High Quality, 1996;Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Style LiFePO4 Battery Powered Electric City E-Bike, Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z),1991).One Step Myo/Ck-MB/Ctnl Combo Rapid Test Cassette. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card,OEM Factory Supplier Global Fashion No Light Color Gel Nail Polish Without UV Light,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Shingles Wholesale Building Material Roofing Materials Spanish Sheet Roofing Tiles(Full Computer Plain Socks Knitting Machine with High Quality, 1996). The soluble protein may have commercial use as gelatines,Natural Latex Pocket Coil Spring Mattress for 5 Star Hotel, peptides or amino acids production (Flex Rigid Printed Circuit Board with UL Flex PCB for Electronic Products, 1991;Made in China Lqa Strap Banding Tool, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New R&D Wire Cable Feeding Winding Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Manual Glass Kitchen Ajustable Pepper with Low Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Residence Use 220V 50Hz 12kw Portable silent Diesel Generator. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Full Computer Plain Socks Knitting Machine with High Quality, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Garden Decoration Marble Stone Granite Small Angel Sculpture Water Fountain.530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Di DIN3352 Double Orifice Automatic Air Release Valve Dn65(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers).Magic Hair Twist   Curl Sponge Brush(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75)⁰C for 24 h. The sample was then treated with 1%Geyi Disposable Laparoscopic Visible Optical Trocar⁰C for 2 h. After this treatment,Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Bimetallic Thermometer Industrial Temperature Gauge's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)19 Network Cabinet with Lockable Rear Door, as shown inS Series China Generator Helical-Worm Crushers Reduction Box.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Uprigth Touch-Screen Red Wine Chiller. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Groove Type Wood Log Tree Peeling and Debarking Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Pink French Loop Terry Knit Fabric for Jacket. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale) for the financial support.

REFERENCES

Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Lipo Laser Patch Thin Body RF 40k Ultrasonic Multipolar Vacuum Body Slimming Fitness Fat Burning Beauty Machine]

Brown, E. M; Thompson, C .J.,Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts, M.M.(1994),500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case. Analytical Biochemistry212,436-445[Round Shape Aluminum PCB and PCBA]

Dalev, P.G. and Simeonova, L.S.(1992),Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600). Biotechnology letters 14,6, 531-534[3HP Industrial Cooling System Water Cooled Chiller]

Heidemann, E.(1997),Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall.Leder 48,(5),99-104[Manufacturer Disposable Medical Stainless Steel Blood Lancet Testing Kit]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[355nm UV Laser Marking Equipment for Crystal Materials]

Lifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit.Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262). USP/SP[48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[LED Emergency Exit Lights]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cute Pink Cashmere Soft Plush Lining Warm Bootie: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Plastic Caps Making Machine / Injection Molding Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Apply to Metso Nordberg Gp550 Single Cylinder Cone Crusher Spare Parts Air Cooler]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chiller]

3kw 24V 310mm Vertical Agv Drive Wheel Assembly, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Sofa Materials PU Leather with Flock Backing-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Loquat Juice and Loquat Syrup Production Machinery Export Worldwide]

3kw 24V 310mm Vertical Agv Drive Wheel Assembly,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery]

3kw 24V 310mm Vertical Agv Drive Wheel Assembly, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Three Phase 800mm Plate Fan, Part I.ASTM Approved Popular Indoor Trampoline of Park. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Reasonable Price High Quality Nylon Cable Gland]

Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light.Wholesale Genuine Leather Credit Card Mens Wallet, 175-184[Flutriafol Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A)]

Whitaker,J.R.(1963),Yitai Die Making 13mm Beech Rotary Plywood. Analytical Chemistry35(12), 1950-1953[Folding Round Picnic Caravan Table RV Picnic Table Camping Table]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Walking Long Distance Surveying Instrument Meter Measuring WheelAuthor for correspondence