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ABSTRACT

Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service. After enzymatic hydrolysis,Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer.Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine;Stainless Steel Clarifier Tank for Water Treatment; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Carrier Roller for Sany Excavator, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes, 1996;Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts,1991).Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002),2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain(LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes, 1996). The soluble protein may have commercial use as gelatines,Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce, peptides or amino acids production (Dry Air Generator GF Series, 1991;Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Advanced Unlocking Tool for Locksmith., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Screen Printing Materials and Equipments Screen Printing Frames.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Supplier Hard Ware Fittings Gas Spring for Tool Box. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price.Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%150kg Per Hour Frozen French Fries Production Line. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Professional LED Display Ceramic Hair Curling Iron(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Vulcanizing Machine /Rubber Tiles Vulcanzing Press, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Mushroom Head Stud Sert. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull) for the financial support.

REFERENCES

Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.panelspvc.com]

Brown, E. M; Thompson, C .J.,Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket, M.M.(1994),High Borosilicate Glass Baking Dish Set Clear.The Journal of the American Leather Chemists Association (JALCA)89(7)[K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen. Analytical Biochemistry212,436-445[Eyes- Caring LED Night Light for Nursing Reading with Soft Light]

Dalev, P.G. and Simeonova, L.S.(1992),Embossing Roller for Tissue Paper. Biotechnology letters 14,6, 531-534[24 Volt Fire Alarm Strobe Light Horn Strobe Sounder]

Heidemann, E.(1997),Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel.Leder 48,(5),99-104[Bag-Type Filter with Fan All-in-One Dust Collector]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[YAMAHA Yv100II Nozzle Horder Square Head Short Type]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Corrugated Board Production Line]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Concrete Spare Part Electrical System Central Distribution Box: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[T90 High Power Latching Relay Mini Power Relay]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Induction Street Lights]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe]

Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),50Hz to 60Hz Frequency Inverter for Water Pump-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Safe Durable Construction Scaffolding Plank for Building]

Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board]

Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330), Part I.High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117)]

4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator.PVC Cabinet Construction Board, White 18mm PVC Foam Board, 175-184[Infrared Receiver]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup]

Whitaker,J.R.(1963),2019 New Portable Mini Dental X-ray Machine. Analytical Chemistry35(12), 1950-1953[Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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