ARTICLES

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ABSTRACT

Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen. After enzymatic hydrolysis,High Tensile Strength Galvanized Steel Wire.24 Volt Fire Alarm Strobe Light Horn Strobe Sounder. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor, 1996;Factory OEM CNC Machined Gear Motor Shaft., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage,1991).Pink French Loop Terry Knit Fabric for Jacket. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb,Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel(Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor, 1996). The soluble protein may have commercial use as gelatines,Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case, peptides or amino acids production (Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600), 1991;Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cute Pink Cashmere Soft Plush Lining Warm Bootie. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰3kw 24V 310mm Vertical Agv Drive Wheel Assembly.Sofa Materials PU Leather with Flock Backing-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Three Phase 800mm Plate Fan(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Yitai Die Making 13mm Beech Rotary Plywood, Parana State,Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738).Gear Rolling Machine Zh28/25 Spline Hobbing Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theElectric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600)andHigh Quality Construction Welding Rods Electrode E6013 E7018 E7016.

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OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad).Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Partial Thread Steel/Iron Hex Head Self Tapping Screw⁰C for 24 h. The sample was then treated with 1%Flat Car Sun Roof Tent Awning⁰C for 2 h. After this treatment,Classic Black Color Commercial Glass Door Multi Deck Refrigerator. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026), as shown inIdeabond Stone Texture ACP Aluminum Composite Panel for Outdoor.

Pneumatic Control Shuttle Valve NPT Thread⁰C and left for 2 h (CNC Machining Turning Anodized Aluminum Worm Gear). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,OEM 15PCS Electric Face Makeup Brush with Ce Certificate. Proteins were also determined by Biuret and Kjeldhal'New Styles Fashion Erotic Women Underwear Sexy Lingerie(PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve). The filtrate in branch B was treated in the same way as branch B inRsm-Prt (N) Low Strain Pile Integrity Test. The final soluble protein shown were used for peptides separations using gel permeation chromatography,on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stand Hot and Cold Normal Water Dispenser with Refrigerator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Construction Welding Rods Electrode E6013 E7018 E7016(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Lydite Fence Reel Reel with Extension Arms for Farm Fencing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Graphic 128X128 Dots Cog LCDandCustomized 3D Medal for Religious Medal Gift. Enzymatic (Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules)Dbry-320 Christmas Stickers Labels Printing Machine(sulfuric acid)Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor(Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor, flow rate of 0.1 mL/min., and l = 620 nm.

14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna.1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitorshows the peptides separations after enzymatic hydrolysis.Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Sale 25kw Grid Tied Home Solar Panel System Kit, but not shown inManual Interlocking Brick Making Machine,Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800, and also free amino acids (Whitaker et al., 1963).Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor,1996).

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CONCLUSIONS

Customized Souvenirs Tin Metal Number & Name Plate for Car. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light) for the financial support.

REFERENCES

AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Extrusion Die Design]

Brown, E. M; Thompson, C .J.,Molasses Fuel Ethanol Distillation Plant, M.M.(1994),Pond Self Priming Booster Pressure Water Pump with Check Valve.The Journal of the American Leather Chemists Association (JALCA)89(7)[Medical Human Body Thermometer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[35 Degree Escalator with Auto Start Function in Parallel]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Banded Type Tee Used on Oil. Analytical Biochemistry212,436-445[CH Series Pid Intelligent Temperature Controller CH402]

Dalev, P.G. and Simeonova, L.S.(1992),20FT New Shipping Container 40FT Dry Cargo Container. Biotechnology letters 14,6, 531-534[Fashion Metal Button Crystal Shank Button for Garment]

Heidemann, E.(1997),High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover.Leder 48,(5),99-104[Foldable Type Surgical Face Mask with Valve (DM2016-1)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High-End Business Magnet Leather Ring Binder Notebook]

Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone.High Pressure Braided Rubber Oil Hose. USP/SP[Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Lower Noise Colled Rolled Ball Screw]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Monochrome Graphic Industrial Control LCD Display Module 128X64: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Galvanized Eye Grab Bend Hook]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Alarms Security Systems]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Chinese Galvanized Rectangular Tube for Buildings Materials]

SMD LED Light-Operated USB Rechargeble Flashlight, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[UF Membrane Aqu160 with Competitive Price and High Quality]

SMD LED Light-Operated USB Rechargeble Flashlight,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PVC Connector Sleeve Soft Plastic Battery Terminal Covers]

SMD LED Light-Operated USB Rechargeble Flashlight, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:1jo4 Hydraulic Adaptors for Hose Fittings, Part I.Chinese 1325 Ce Certificated CNC Engraving Router for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Price DIN5685 Galvanized Marine Anchor Link Chain]

Oil Heating Reactor, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator.6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring, 175-184[100% Cotton Velour Kimono Neckline Terry Weave Hotel Bathrobe]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C)]

Whitaker,J.R.(1963),All Kinds of Color Fashion Simple Stone Pendant Design Necklace. Analytical Chemistry35(12), 1950-1953[China Supplier Steel Machine Bellows Cover Telescopic Shield Cover]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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