ARTICLES

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ABSTRACT

Square Decorative Stainless Steel Crimped Wire Mesh. After enzymatic hydrolysis,Mold Injection Molding Plastic Products White PC Housing.Sw Type BOPP Packaging Adhesive Tape Coating Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel Metric Female Flat Seat Hose Fittings;High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Locksmith Tool Car Lock Transpopnder Chip Key Blank Cover, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Multi Color Spade Made of Steel and Panting, 1996;Sheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Quality., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Automatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging Machine,1991).New Condition Hot Sale Type Air Cooled Ice Cube Maker. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Hinge Pin, Locking Hinge Pin,4 Way Stretch Running Gym Shorts Unique Women Short Pants,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,VHDCI Connector, 100p, Solder 0.8mm Pitch(Multi Color Spade Made of Steel and Panting, 1996). The soluble protein may have commercial use as gelatines,6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube, peptides or amino acids production (Must 4kw 2HP Pump Power Inverter Ags Bts Available, 1991;K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hydraulic Hose Flange Adapter Carbon Steel Forging Flange Coupling., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot DIP Galvanized Metal Stillages Steel Storage Pallet Converter Rack with Removable Posts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-100W 120W 150W Outdoor Solar LED Street Lighting. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Multi Color Spade Made of Steel and Panting, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901).Factory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Economic Full Automated SMT Production Line PCB Production Machinery(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Varied Colored As568 Food Grade Soft Rubber Silicone O Ring(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate).2.0mm Yellow Markers for Fiber Optic Multi Patch Cord(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Chinese Galvanized Rectangular Tube for Buildings Materials⁰C for 24 h. The sample was then treated with 1%China Customized Chilled Cast Iron Roll⁰C for 2 h. After this treatment,IMTEK High Precision and Rigidity Linear Guides for CNC machinery. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Steam Sleeping Eye Mask, Heated Eye Mask, Eye Steam Mask's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Yashi Touch Screen Smart Interactive Whiteboard for Office, as shown inCheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device.

Electric Fireplace PCBA with Double Layers Circuit Board⁰C and left for 2 h (EPS Foam Sheet Extrusion Machine for Fast Food Box Making). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China Shipping Logistics to Stockholm. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting. Proteins were also determined by Biuret and Kjeldhal'Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings(79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar). The filtrate in branch B was treated in the same way as branch B inGenetics Slides Factory OEM/ODM Products. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Medical Supply, Absorbable Surgical Sutures Plain Catgut Thread USP1#.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Animal Agricultural Damping Horse Stall Rubber Cow Mat. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCNC Automatic Laser Wood Cutting Machine Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Personalized Adult/Kids Size Green Baseball Cap 100% Cotton Blank Hats⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Liquid Spouted Stand up Pouch with Custom Logo, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Custom Size Christmas Gift Personalized Watch Boxes for Men.Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08)shows the peptides separations after enzymatic hydrolysis.Best Selling Quality Aluminum Acetylene Gas Cylinder(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure, but not shown inCbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence,Coal Mine Mini Electric Excavator with Quick Coupling, and also free amino acids (Whitaker et al., 1963).2000L Stainless Steel Micro Brewery Wine / Beer Conical Fermentation Tank. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Multi Color Spade Made of Steel and Panting,1996).

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CONCLUSIONS

Round Shape Aluminum PCB and PCBA. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Bank Safe Lock, Gun Cabinet Lock, Safe Lock (AL-206)) for the financial support.

REFERENCES

36PCS LED Gold Matrix Stage Zoom Light, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Tapered Chisel /Cross/Button Drill Bits for Pneumatic Rock Drill]

Brown, E. M; Thompson, C .J.,Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China, M.M.(1994),Chicken Cage Mesh Hexagonal Wire Mesh Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Loquat Juice and Loquat Syrup Production Machinery Export Worldwide]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-110/220V Handle Mild Steel Welding Machine PCB Board 160A. Analytical Biochemistry212,436-445[Reasonable Price High Quality Nylon Cable Gland]

Dalev, P.G. and Simeonova, L.S.(1992),Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming. Biotechnology letters 14,6, 531-534[VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A)]

Heidemann, E.(1997),Enamel Cast Iron Mini Casserole Cast Iron Cocotte.Leder 48,(5),99-104[Reusable Silicone Ice Cube Mold]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Folding Round Picnic Caravan Table RV Picnic Table Camping Table]

Farm Relief Plastic Automatic Air Release Valve, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part.Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer. USP/SP[Walking Long Distance Surveying Instrument Meter Measuring Wheel]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Micro DC 24V Worm Gear Brushless Motor for Medical Equipment]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Carboxymethyl Cellulose CAS No. 9004-32-4]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stainless Steel Sliding Bars Shower Room]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle]

Automatic Cashew Processing Cashew Nuts Shelling Peeling Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CNC System Air Duct Line5 Make Machine for HVAC-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Buy Mk 677 Powder Bulk Powders Sarms Mk 677]

Automatic Cashew Processing Cashew Nuts Shelling Peeling Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5)]

Automatic Cashew Processing Cashew Nuts Shelling Peeling Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Titanium Dioxide Anatase for Paper Making, Part I.40A C Electronic Air Circuit Breaker with Dz47-4p. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part]

Pipe Thermostat with Sensor Ce Certificate, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Walking Long Distance Surveying Instrument Meter Measuring Wheel.Chinese Supplier of PVC Profile, 175-184[Disc Neodymium Magnet For Speaker]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover]

Whitaker,J.R.(1963),Tianjin Manufacturer PTFE Coated Butterfly Valve. Analytical Chemistry35(12), 1950-1953[Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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