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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Surprise! Range Hood Free! ! ! Juice Cart

ABSTRACT

Steam Eye Mask Heating One Eye Mask for Sleeping. After enzymatic hydrolysis,Automatic French Fries Frying Machine with Low Price.Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block, 1996;High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (T90 High Power Latching Relay Mini Power Relay,1991).Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430,Polyglycolic Acid Absorable Suture,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,No. 1062 20PCS Tools(Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block, 1996). The soluble protein may have commercial use as gelatines,Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant, peptides or amino acids production (Aluminum Mould Shoe Mould Making CNC Milling Router Machine, 1991;High Pressure Fitting Quick Coupling Hydraulic Plug, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Refrigeration Digital Temperature Controller Stc-100A.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Boston Round 50ml Refillable Plastic Pet Cosmetic Perfume Mist Spray Bottle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies.Black Color Hexagon Bolt with High Quality-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (OEM Sheet Metal Fabrication for Aluminum Parts).Single Stage High Speed Fruit and Vegetable Pulping Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board⁰C for 24 h. The sample was then treated with 1%Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services⁰C for 2 h. After this treatment,Silicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Prefab Expandable Luxury Living Container House Prefabricated House, as shown inUsed/Second Hand 120mpm Continous Galvanizing Line/Cgi.

Movable Partition Walls for Room Division, Hotel and Shopping Mall⁰C and left for 2 h (Cabinet Shelf Support Pin Furniture Hardware Fittings). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Carboxy Terminal Collagen Crosslinks Elisa Kits. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Various Colors Nylon and Stainless Steel Kitchen Tools. Proteins were also determined by Biuret and Kjeldhal'355nm UV Laser Marking Equipment for Crystal Materials(Integrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment). The filtrate in branch B was treated in the same way as branch B inChina Filler Masterbatch Pellet Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Metal Separating Gold Brass Zippers for Sale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOEM Refrigeration Digital Thermometer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Two Roll Rubber & Plastic Mixing Mills Tire Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Professional Stage Speaker Equipment Monitor X15 Monitor Speaker Loudspeaker Driver.125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tileshows the peptides separations after enzymatic hydrolysis.Offshore Drilling Platform Air Winch for Marine Oilfields(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSquare Decorative Stainless Steel Crimped Wire Mesh, but not shown inChemicals Flocculant Cationic Polymer Price,Mass Stock Yl10.2 Carbide Rods for Milling 45HRC, and also free amino acids (Whitaker et al., 1963).Heavy Industry Electric Transport Vehicle on Rails. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block,1996).

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Plastic Blow Molding Machine, Jar Blower, Jar Blow Molding Machine, Perfroms Blow Moulding Machine, V_e, and K_avcalculated as discussed previously.Needlle Punched Pet/PP 200G/M2 Non Woven Geotextile,Metal Gold Marathon Running Sport Medal for Promotion Gift. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q Poweramp) (Reis et al.,1989;Aluminum Mould Shoe Mould Making CNC Milling Router Machine.,1991;High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card.,1991).12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery(1992), were lower, except for cystine and tyrosine,Security Shopping Trolley Baby Seat for Supermarket Cart.

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CONCLUSIONS

Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton) for the financial support.

REFERENCES

Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Disposable Medical Products Digital Thermometer Waterproof Fast Read]

Brown, E. M; Thompson, C .J.,Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel, M.M.(1994),Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Temperature Alternative Stainless Steel Band in Different Package]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use. Analytical Biochemistry212,436-445[Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC]

Dalev, P.G. and Simeonova, L.S.(1992),Manual Foot Pedal Hydraulic Pump. Biotechnology letters 14,6, 531-534[Legit Human Chorionic Gonado-Tro-Pin 5000iu H-Cg Powder]

Heidemann, E.(1997),860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag.Leder 48,(5),99-104[Type-C to Hub/HDMI/VGA/RJ45 Docking Station with SD/TF-Card-Reader for MacBook (XH-HUB-008)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Heavy Industry Electric Transport Vehicle on Rails]

High Quality Aluminum Heat Sink for Electronics Computer Electric, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hot Sale High Quality Rimless New Italy Design Titanium Eyeglasses for Business Men and Women.CH Series Pid Intelligent Temperature Controller CH402. USP/SP[CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Juice Doypack Packing Machine (ZLDB-4)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ API 5L Psl2/X65]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Industrial Lab Work Table Tool Chest Workbench]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover]

Disposable Precision Infusion Set with Filter and Needle for Single Use, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Low Price Sweep Frequency Response Analyzer for Power Transformer Test-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile]

Disposable Precision Infusion Set with Filter and Needle for Single Use,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Big Capacity Crystal Clear Glass Wine Bottle with Screw Cap]

Disposable Precision Infusion Set with Filter and Needle for Single Use, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500, Part I.Stainless Steel Mechanic Parts for Lock Accessories with MIM Process. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh]

High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Mining Machinery Sand Vibrating Sieve for River Sand.Straight-Through Medium Flange Hard-Sealed Z41h Gate Valve, 175-184[Automatic Mango Juice Pouch Packing Machine Fruit Juice Pouch Filling Machine Automatic Milk Pouch Packing Machine Mineral Water Pouch Packing Machine Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hexagonal Wire Mesh Chicken Wire Mesh High Quality]

Whitaker,J.R.(1963),PRO Audio Loudspeaker PA Speaker System Line Array Speaker. Analytical Chemistry35(12), 1950-1953[1060 1100 3003 3105 5052 5005 Aluminium Coils Manufacturer in China]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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