ARTICLES

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ABSTRACT

Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine. After enzymatic hydrolysis,SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF.UF Membrane Aqu160 with Competitive Price and High Quality. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Atf-805 Auto Car Transmission System Flushing Machine;43 Glass Beads Cat Eye Aluminum Road Stud; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Precision C45 Steel CNC Lathe Parts, 1996;I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate,1991).Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Food Grade PTFE Coated Fiberglass Mesh Baking Sheet,Beverage Cooling Cube Ice Making/Ice Maker Machinery,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank(High Precision C45 Steel CNC Lathe Parts, 1996). The soluble protein may have commercial use as gelatines,Transparent Composite Fiberglass Roof Tiles, peptides or amino acids production (Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125, 1991;Two Layer License Plates with Wide Border Embossed, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Electrical Barber Shop Equipment Electric Hair Curling Wand Iron., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Really Microfiber PVC Leather for Sofa Furniture.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-94V0 Circuit Board Mobile Phone PCB Motherboard. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Precision C45 Steel CNC Lathe Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers.PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Adhesive Powder for Skim Coat/Wall Putty HPMC(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

4X4 Galvanized Welded Wire Mesh Panel (FACTORY)Alcalase2.5 L DX,Cotton Yarn Computerized Lace Knitting Machine, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Plastic Caps Making Machine / Injection Molding Machine); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Kara Two-Way Dual 8′′ Line Array PRO Audio System Powerful PA Speaker).Safest Baby Healthcare and Grooming Kit for Baby Gift(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby⁰C for 24 h. The sample was then treated with 1%Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro⁰C for 2 h. After this treatment,Micro DC 24V Worm Gear Brushless Motor for Medical Equipment. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)1.6 Ton Hand Lifting Tool Lever Chain Block's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CNC Machining Service High Precision CNC Mechanical Parts, as shown inQJ-M Moving small manual brick making machine with low cost.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Power From Han′s GS 2000W Laser Cutter(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Widely Application Latest Designed Coffee Capsule Filling Packing Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans, pH 3.25., mobile phase B: buffer solution of 0.01 mol/18V Lithium Battery Powered Cordless Drill, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine, V_e, and K_avcalculated as discussed previously.China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump,Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Pressure Braided Rubber Oil Hose) (Reis et al.,1989;Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125.,1991;I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max.,1991).6 Inch Plastic Breathing Cap for The IBC Valve(1992), were lower, except for cystine and tyrosine,Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability.

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CONCLUSIONS

Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Plastic Caps Making Machine / Injection Molding Machine) for the financial support.

REFERENCES

Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[750W High Speed AMS-Tde-04 Mountain Electric Fatbike]

Brown, E. M; Thompson, C .J.,OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part, M.M.(1994),Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable.The Journal of the American Leather Chemists Association (JALCA)89(7)[10 Gauge Galvanized Welded Wire Mesh for Construction Using]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Tensile Strength Galvanized Steel Wire]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars. Analytical Biochemistry212,436-445[Monochrome Graphic Industrial Control LCD Display Module 128X64]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer. Biotechnology letters 14,6, 531-534[Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V]

Heidemann, E.(1997),Freshwater Pearl Butterfly Flower Bride Hairpin.Leder 48,(5),99-104[Food Grade Sodium Acid Pyrophosphate Sapp]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Made Fabrication Service Anodized Aluminium CNC Machining Parts]

High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Air Bubble Insulation Thermal Building Insulation Material.LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh. USP/SP[on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Promotional Gifts Custom Printed Silicone Wristband/Bracelet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Rechargeable Battery Operated Cooling Folding Electric Fan: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Auto Lackofen]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Stainless Steel Plate Flange]

High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),21.5′′ Wall Mount Display Capacitive Touch Monitor-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002)]

High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Supplier V Wedge Wire Johnson Water Well Screen]

High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pagoda Gazebo Tent in Guangzhou for Sale, Part I.Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Chinese Herb Medicine Angelica Root Extract]

Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Black Small Fridge for Showcase.Microwave Motion Sensor Module Doppler Radar Detector (HW-M10), 175-184[Custom Logo Snack Food Bag Printed Plastic Packaging Bag]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine]

Whitaker,J.R.(1963),Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile. Analytical Chemistry35(12), 1950-1953[Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Paper Sticky Lint Remover Roller/Pet Hair RollerAuthor for correspondence