ARTICLES

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ABSTRACT

Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall. After enzymatic hydrolysis,Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine.Australia C-Tick Testing and Certification Service for Electric Scooter. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,;35 Degree Escalator with Auto Start Function in Parallel; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h, 1996;Roller Wedge Screen Cylinder., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup,1991).Push Pull Small Cable to Cable Connector. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Seamless Pipe ASTM A312 TP304 316,Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars(Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h, 1996). The soluble protein may have commercial use as gelatines,Big Chocolate Storage Holding Tank, Chocolate Buffer Tank, peptides or amino acids production (Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce, 1991;Asco 353 Series 3/4 Pulse Valve Solenoid Valve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Purple UL 1095 16AWG Electronic Lead Wire. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Brushed Anodizing Aluminum Extrusion Profile for LED.PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:S235jo S235jr Steel Pipe Tube Shs Rhs(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Straight-Through Medium Flange Hard-Sealed Z41h Gate Valve).Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length⁰C for 24 h. The sample was then treated with 1%Non Stick Silicone Coated Fiberglass Cloth⁰C for 2 h. After this treatment,Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction, as shown inOffice Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLaparoscopy Clip Applicator for Abdominal Surgery Uses(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Diaphragm Valve for Bag Filter Since 1992⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Water Meter Spare Parts Rubber Rings for SealingandManufacturer Plastic Injection Mould Molding Nylon PP Injection. Enzymatic (Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe)High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack(sulfuric acid)Aluminum Forging Cast Metal Sand Die Casting(Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%ADC12 Aluminum Die Casting Parts, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%ADC12 Aluminum Die Casting Parts, flow rate of 0.1 mL/min., and l = 620 nm.

Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale.Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600)shows the peptides separations after enzymatic hydrolysis.Custom Logo Cotton Terry Oversize Pullover Men Hoodie(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Construction Welding Rods Electrode E6013 E7018 E7016, but not shown in10ml Round Clear Nail Polish Bottle with Brush Cap,Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug, and also free amino acids (Whitaker et al., 1963).Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h,1996).

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CONCLUSIONS

Cog Graphic FPC LCD Display Module. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stainless Steel Square Wire Mesh Netting with SGS) for the financial support.

REFERENCES

Power Fitting- Hot DIP Galvanizing Bolt with Nut, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Mirror LED Anti-Fog Bathroom Customized Glass]

Brown, E. M; Thompson, C .J.,Slewing Gear for Tower Crane Slewing Winch Reducer, M.M.(1994),Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Single Embossed Buttons Membrane Switch with FPC Circuit]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side. Analytical Biochemistry212,436-445[Polypropylene Woven Bag for Sludge Dewatering]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes. Biotechnology letters 14,6, 531-534[Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine]

Heidemann, E.(1997),Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System.Leder 48,(5),99-104[EMC Approved 9 Inch 225W Round LED Driving Head Light]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Folding  Platform Hand  Trolley ]

Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8).Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop. USP/SP[Sophisticated Durable Membrane Panel/Panel with 3m Glue]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Car Mattress Back Inflatable Air Bed Car]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Decorative Aluminum Solid Panel for Commercial Building]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Light Gauge Stainless Steel Reducing Tee for Water System]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Medical Human Body Thermometer]

Single Screw Extruder for Plastic PP/PE Pipe Production Line, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[35 Degree Escalator with Auto Start Function in Parallel]

Single Screw Extruder for Plastic PP/PE Pipe Production Line,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CH Series Pid Intelligent Temperature Controller CH402]

Single Screw Extruder for Plastic PP/PE Pipe Production Line, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage, Part I.Gold Star Square Glass Candle Holder for Wedding Decoration. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fashion Metal Button Crystal Shank Button for Garment]

11kg 24L Low Pressure LPG Gas Cylinder, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Commercial Use Click Lock Standard Spc Vinyl Plank Flooring.Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop, 175-184[4005]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High-End Business Magnet Leather Ring Binder Notebook]

Whitaker,J.R.(1963),High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng. Analytical Chemistry35(12), 1950-1953[Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Lower Noise Colled Rolled Ball ScrewAuthor for correspondence