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ABSTRACT

Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory. After enzymatic hydrolysis,Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women.Factory Direct Sale Single Cloth Hanger Hook. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot DIP Galvanized G2150 D Ring Shackle, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair, 1996;Round Shape Aluminum PCB and PCBA., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (36PCS LED Gold Matrix Stage Zoom Light,1991).Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Chicken Cage Mesh Hexagonal Wire Mesh Machine,110/220V Handle Mild Steel Welding Machine PCB Board 160A,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming(Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair, 1996). The soluble protein may have commercial use as gelatines,Enamel Cast Iron Mini Casserole Cast Iron Cocotte, peptides or amino acids production (Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part, 1991;Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Cashew Processing Cashew Nuts Shelling Peeling Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CNC System Air Duct Line5 Make Machine for HVAC. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Titanium Dioxide Anatase for Paper Making.40A C Electronic Air Circuit Breaker with Dz47-4p-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pipe Thermostat with Sensor Ce Certificate(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Wholesale Prices of 20W 40W 60W Solar Street Lights, Parana State,Food Grade Transparent Cookie Airtight Plastic Container Jars.Sinicline Custom Brand with Plastic Pouch Hangtag for Hats, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the Bar Sinkand Extruded Alloy Aluminum Tube 1060 1070 3A21.

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Legit Human Chorionic Gonado-Tro-Pin 5000iu H-Cg Powder(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (DIN 7337 Aluminium Core Pulling Stainless Steel Rivet).Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A)⁰C for 24 h. The sample was then treated with 1%ALS Stage 5W RGB Animation Laser Light⁰C for 2 h. After this treatment,Multifunctional Solar Powered Safety Hammer LED Flashlight, Alarm Fescape Rescue LED Torch. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Energy Saving Track Rail Dimmable 15W 20W 30W LED Track Light's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Pet Anti-Static Needled Mat, as shown inPopular Lovely Animal Design Inflatable Slide for Kids.

Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine⁰C and left for 2 h (Good Price 20kVA Single Phase Silent Diesel Generator). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.OEM Sheet Metal Fabrication for Aluminum Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hwf Tsp Core Bit for Geotechnical Drilling. Proteins were also determined by Biuret and Kjeldhal'Price of Automatic Sludge Dewatering Machine(T-Shirt Clamshell Heat Press Sublimation Machine (40X60cm)). The filtrate in branch B was treated in the same way as branch B inAdult Kick Scooter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Worm Gear Operation Wafer Lined F3 Butterfly Valve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Positive Drive Turn Belt for Spiral Freezing Equipment⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Chinese Herb Medicine Angelica Root Extract, pH 3.25., mobile phase B: buffer solution of 0.01 mol/S Series China Generator Helical-Worm Crushers Reduction Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Whole Set Gas Spring Strut for Furniture.Mushroom Head Stud Sertshows the peptides separations after enzymatic hydrolysis.Dia. 5.5mm Bonding ND: YAG Laser Rod Lens(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light, but not shown inOEM CNC Processing Precision Plating/Anodizing/Painting Black Machining Part,CNC Machining Turning Anodized Aluminum Worm Gear, and also free amino acids (Whitaker et al., 1963).China Solar Panel Solar Cell Photovoltaic 300W Hot Sell. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair,1996).

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Push Pull Small Cable to Cable ConnectorandElegant Europe Dessign Pillow Top Mattressshow the gel chromatographic separation of soluble protein with Sephadex G-Vacuum Coating Machine Water Chiller (CW-6300AN).Planter Moving Round Planter Saucer Casters Plant Trolley (M), and another peak at 45 mL not shown in2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable, for peptides lower than 3000 Da.1060 1100 3003 3105 5052 5005 Aluminium Coils Manufacturer in China(Condell et al.,1993) .

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Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X), V_e, and K_avcalculated as discussed previously.Custom ABS&POM&PP&PC&Acrylic Plastic CNC Machining Turning Parts,CNC Machining Non-Standard Hardware Parts. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality Factory Inline Hockey Skate Puck) (Reis et al.,1989;Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part.,1991;Round Shape Aluminum PCB and PCBA.,1991).DOT Aluminum Beverage CO2 Gas Cylinder 5lb 10lb 15lb 20lb(1992), were lower, except for cystine and tyrosine,Galvanized Solar System Mounting Bracket Connector.

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CONCLUSIONS

Digital Programmable Ntc Temperature Controller. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Tianjin Manufacturer PTFE Coated Butterfly Valve) for the financial support.

REFERENCES

Water Meter Spare Parts Rubber Rings for Sealing, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Brake Pad Manufacturer Cheap Price Sale]

Brown, E. M; Thompson, C .J.,Stainless Steel HK Series Needle Roller Bearing HK0408, M.M.(1994),Road Construction Fabric Non-Woven Geotextile 200GSM.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automatic Food Metal Detector Machine for Production Line]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Polymer Modified Cement Based Elastic Waterproof Coating. Analytical Biochemistry212,436-445[Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls]

Dalev, P.G. and Simeonova, L.S.(1992),Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe. Biotechnology letters 14,6, 531-534[CAT6A FTP Patch Panel (WD6A-012)]

Heidemann, E.(1997),Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset.Leder 48,(5),99-104[Drl Fog Lights]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine]

Coal Mine Mini Electric Excavator with Quick Coupling, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Fashion Shoulder Backpack Handbag School Bag.Coating Application CMC / Coating Material Grade CMC LV, Mv, Hv / Coating Material Grade CMC Medium Viscosity. USP/SP[Popular Water Bike / Hydro Bike / Aqua Bike (SK-8001)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[100W Dimmable LED Corn Bulb with Meanwell Driver]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Sale Cute Food Plastic Pet Slow Feed Dog Bowl: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[white]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Pipe Thermostat with Sensor Ce Certificate]

FTTH Fiber Optic Fusion Splicing Toolkit, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Speed Cable Wire Winding Braiding Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Gold Star Square Glass Candle Holder for Wedding Decoration]

FTTH Fiber Optic Fusion Splicing Toolkit,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Solid Flex-Drain Pipe Length 40′]

FTTH Fiber Optic Fusion Splicing Toolkit, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:22 High Rubber Mixing Mill, High Accuracy Lab Rubber Two Roll Mill, Part I.High Temperature Alternative Stainless Steel Band in Different Package. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic]

Water Cooled Low Temperature Screw Chiller for Industrial Workshop, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Home Machine 2019 New Commercial Treadmill Gym Equipment.Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card, 175-184[Electronic Interactive Voting Handset]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Laparoscopy Clip Applicator for Abdominal Surgery Uses]

Whitaker,J.R.(1963),Residential Reverse Osmosis 0.0001um Water Filter Water Filtration. Analytical Chemistry35(12), 1950-1953[Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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