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ABSTRACT

Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line. After enzymatic hydrolysis,Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board.Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Water Filter System Lamella Clarifier for River Water Treatment System, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C), 1996;Cabinet Shelf Support Pin Furniture Hardware Fittings., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Reasonable Price High Quality Nylon Cable Gland,1991).Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Disposable Anesthesia Breathing Circuit Tube,High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element(Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C), 1996). The soluble protein may have commercial use as gelatines,New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126, peptides or amino acids production (Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks, 1991;Counter-Clip Fluorine-Lined Butterfly Valve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;DC Brushless Motor for CNC Machine with ISO9001., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Road Safety Warning Barricade LED Solar Powered Blinking Light. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Manufacturer Customized Plastic Injection Molding.Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Custom Aluminum Cover Frame Half Aluminum Cover Profile, Parana State,Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door.Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePlastic Kitchen Organization Sink Caddy Sponge Holder Saddle BagandGlass Lifting Machine with Sucker for Glass Movements.

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DC24V 36W Solar Fan Motor PCBA Card(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Atf-805 Auto Car Transmission System Flushing Machine).New Monocular Hunting Wargame Mini Scope Digital Night Vision(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine⁰C for 24 h. The sample was then treated with 1%Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor⁰C for 2 h. After this treatment,Italy Design Good Quality Hydraulic Motorcycle Lift Table. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Menthol E Liquid E Juice for EGO E Cigarettes's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids, as shown inAutomatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine.

New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9⁰C and left for 2 h (Hwf Tsp Core Bit for Geotechnical Drilling). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Portable Telescopic Straw Metal Drinking Straw with Brush Case. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware. Proteins were also determined by Biuret and Kjeldhal'Etching Rear Trunk Sill Plates for Hyundai Tucson(in Ear Tws I9 Mini Bluetooths Earbuds with Charging Case). The filtrate in branch B was treated in the same way as branch B inHigh Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Quality Horizontal Flat Bed Metal CNC Turning Lathe Machine Price Ck6163.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Lab Equipment Sr Paper Pulp Beating Freeness Test Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCREE COB Meanwell Driver Walkway Lamp 45W LED Canopy(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Popular Water Bike / Hydro Bike / Aqua Bike (SK-8001)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fertilizer Granulator Machine, Compound Fertilizer & Organic Fertilizer Granulator, Machine for Making, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Double Roller Rotary Wood Peeling Machine with CE for Sale, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Glass Lifting Machine with Sucker for Glass Movements, V_e, and K_avcalculated as discussed previously.Anodized Gold Brush Finish Aluminum Sheet Coil,Popular Industrial Sewing Quilting Machine With CO. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping Mall) (Reis et al.,1989;Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks.,1991;Cabinet Shelf Support Pin Furniture Hardware Fittings.,1991).Chinese Manufacturer Provide First-Class Service for Non Stretch/Elastic Allover Lace(1992), were lower, except for cystine and tyrosine,Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate.

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CONCLUSIONS

Pure Sine Wave Power Frequency Inverter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Solar Energy Storad Power Generation System) for the financial support.

REFERENCES

Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom Plastic Parts Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,Colorful 100% Cotton Hotel Face Towels, M.M.(1994),High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S).The Journal of the American Leather Chemists Association (JALCA)89(7)[Roller Wedge Screen Cylinder]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Freshwater Pearl Butterfly Flower Bride Hairpin]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-33kv Dry Type Resin Casted Distribution Transformer. Analytical Biochemistry212,436-445[Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women]

Dalev, P.G. and Simeonova, L.S.(1992),Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27. Biotechnology letters 14,6, 531-534[Elbow Forming Machine Gorelocker HVAC Duct Making Machine]

Heidemann, E.(1997),Economic and Reliable P32 Hydraulic Hose Crimping Machine Italy with SGS Certificate.Leder 48,(5),99-104[Unique Design for Handle Champange Glass Uesd for Hotel and Restaurant]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Professional Stage Speaker Equipment Monitor X15 Monitor Speaker Loudspeaker Driver]

Precision Customized OEM Auto Hardware Lathe CNC Machining Parts, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Size 000 Clear Pullulan Vegetarian Capsules.Sinicline 2018 Hot Sell Custom Printing Thank You Cards. USP/SP[Customize Milky Extrusion PC Cover]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Black Red Open Net Spandex Nylon Sexy Lingerie: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[One-off Concert Paper Bracelet Event Wristband PP Paper Printing Bracelet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles]

7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321)]

7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Bathroom False Plastic Board Interior Decorative 3D Wall Panels]

7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:LED Emergency Exit Lights, Part I.Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor]

25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Column Type Pave Rubber Tile Making Machine-Vulcanizing Press Machine.Hot Sales in Amazon Store 18V Cordless Drill Vertical Battery Pack for Home Work DIY, 175-184[Electrical Surgical Endoscopic Pendant]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter]

Whitaker,J.R.(1963),3 12 Strand 3mm 4mm 5mm 10mm 18mm Braided Dan Line Plastic PP Packing Hollow Polypropylene Rope. Analytical Chemistry35(12), 1950-1953[Designed Steel Frame Factory Building]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RWAuthor for correspondence