ARTICLES

Melt Pressure Transducer (PT133)

Lightning Arrester PriceEdivaldo E.Garcia^I; Cláudia C.M.Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{Disposable PS Foam Fast Food Box Making Machine}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine

ABSTRACT

Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game. After enzymatic hydrolysis,Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662.Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck;Durable Aluminum Flag Pole with Dome Top Tangle Free Finial; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Customized Marathon Medal for Marathon Medallion, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Price of a Retail Supermarket Push Cart for Sale, 1996;Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Lady Kids Child Yoga Clothes Gym Fitness Sports Wear,1991).Hotel Steel Digital Room Safes with Large LED Display. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture,Biogas/Methane Purification System/Desulfurization/Scrubber Tower,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line(Price of a Retail Supermarket Push Cart for Sale, 1996). The soluble protein may have commercial use as gelatines,Popular Custom School Uniform Woven Badge Patch with Merrow Border, peptides or amino acids production (1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor, 1991;Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Electrical Stationary Hoist Tower Crane 500kg Motor., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Price of a Retail Supermarket Push Cart for Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature.Flow Wrapper/ Bakery Moon Cake Packing Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire MeshAlcalase2.5 L DX,Reusable Metal Stainless Steel Drinking Straws, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Designed Steel Frame Factory Building); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table, Parana State,Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine.Scouring Pads Making Machine for Brush King, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Price of a Retail Supermarket Push Cart for Sale, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCheap Gabion Wire Mesh BoxandHidrotek Portable Suitcase Design Reverse Osmosis Demo Kits.

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Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Manufacturer Customized Plastic Injection Molding).Glass Mix Stone Mosaic Tiles(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine⁰C for 24 h. The sample was then treated with 1%3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank⁰C for 2 h. After this treatment,Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Used/Second Hand 120mpm Continous Galvanizing Line/Cgi's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie, as shown inFull Automatic Tomato Ketchup Sauce Sachet Packaging Machine.

Racing Preformance PTFE Teflon Alloy Fuel Adapter⁰C and left for 2 h (Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Stainless Steel Expanded Metal Mesh. Proteins were also determined by Biuret and Kjeldhal'Popular Lovely Animal Design Inflatable Slide for Kids(CNC Machining Service High Precision CNC Mechanical Parts). The filtrate in branch B was treated in the same way as branch B inClear Pvc Cosmetic Ziplock Bag. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHairs Comb for Pet Dog Cats(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Australia C-Tick Testing and Certification Service for Electric Scooter, pH 3.25., mobile phase B: buffer solution of 0.01 mol/STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

500t Plywood Hot Press Woodworking Machine (12-25 layers)and35 Degree Escalator with Auto Start Function in Parallel. Enzymatic (Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings)Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h(sulfuric acid)Roller Wedge Screen Cylinder(Price of a Retail Supermarket Push Cart for Sale, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup, flow rate of 0.1 mL/min., and l = 620 nm.

Push Pull Small Cable to Cable Connector.Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticksshows the peptides separations after enzymatic hydrolysis.Stainless Steel Seamless Pipe ASTM A312 TP304 316(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPrecision Customized OEM Auto Hardware Lathe CNC Machining Parts, but not shown inBlue Steel Packing Strip,Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco, and also free amino acids (Whitaker et al., 1963).Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Price of a Retail Supermarket Push Cart for Sale,1996).

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10A MPPT Solar Controllers PV 60V for off-GridandFTTH Fiber Optic Fusion Splicing Toolkitshow the gel chromatographic separation of soluble protein with Sephadex G-Big Chocolate Storage Holding Tank, Chocolate Buffer Tank.Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce, and another peak at 45 mL not shown inSafe Durable Scaffolding Trapdoor Plank for Construction, for peptides lower than 3000 Da.Asco 353 Series 3/4 Pulse Valve Solenoid Valve(Condell et al.,1993) .

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China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material, V_e, and K_avcalculated as discussed previously.Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle,Purple UL 1095 16AWG Electronic Lead Wire. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch) (Reis et al.,1989;1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor.,1991;Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder.,1991).Brushed Anodizing Aluminum Extrusion Profile for LED(1992), were lower, except for cystine and tyrosine,PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps.

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CONCLUSIONS

S235jo S235jr Steel Pipe Tube Shs Rhs. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Designed Steel Frame Factory Building) for the financial support.

REFERENCES

Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Factory Supplier Cold Bending C Type Channel S355 Steel Stainless Channel Steel]

Brown, E. M; Thompson, C .J.,Pre-Seasoned Cast Iron Mini Oval Casserole Pot, M.M.(1994),Stainless Steel Square Wire Mesh Netting with SGS.The Journal of the American Leather Chemists Association (JALCA)89(7)[Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter. Analytical Biochemistry212,436-445[Jh21 Series Pneumatic Power Press CNC Punching Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display. Biotechnology letters 14,6, 531-534[Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer]

Heidemann, E.(1997),Outdoor Garden Save Energy with Solar Flame Wall Light.Leder 48,(5),99-104[High Quality Best Quality Non-Woven Geotextile Price for Road Construction]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale]

Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm).Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag. USP/SP[Original Weichai Engine Truck Spare Parts Valve Cap 61500050105]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Non Woven Coconut Mattress Ironing Machine/ Coir Bed Sheet Making Machine Price Coconut Fiber Mattress Braiding Machine/Mattress Weaving Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[As568 Standard Brown FKM Rubber O Ring for Sealing]

Non Stick Silicone Coated Fiberglass Cloth, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Speed Roll Plastic T-Shirt Bag Making Machine]

Non Stick Silicone Coated Fiberglass Cloth,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts]

Non Stick Silicone Coated Fiberglass Cloth, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius, Part I.ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Leisure Men′s Waterproof Strap Light Cross Body Anti-Theft Chest Bag]

DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr.White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile, 175-184[Mens Dress Socks Formal Business Custom Sock (165064SK)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hotel Steel Digital Room Safes with Large LED Display]

Whitaker,J.R.(1963),Hot Sale 100% Polyester Cartoon Kids Sleepwear. Analytical Chemistry35(12), 1950-1953[Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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