ARTICLES

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ABSTRACT

24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum. After enzymatic hydrolysis,Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine.Single Embossed Buttons Membrane Switch with FPC Circuit. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions, 1996;Large Slewing Rings Bearings for Wind Turbine Ring on Sale., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container,1991).Chemicals Flocculant Cationic Polymer Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ycom USB Charger and Data Cable with Aluminum for Type C,New Design Inflatable Water Playground Amusement Park with Water Slide Pool,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface(Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions, 1996). The soluble protein may have commercial use as gelatines,Light Weight Polyester Eye Mask for Travel Airplane Hotel, peptides or amino acids production (6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8), 1991;2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Bamboo Tablet Stand Laptop Rack Bt-2212., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Personal Care Glutathione Skin Whitening Tablets.Customized Over Size Boys Hoodie with Embroidered-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Digital Printing Football Fans Flag(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine, Parana State,Car Mattress Back Inflatable Air Bed Car.Promotional Plastic Laminated Custom Shopping PP Woven Bag, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAluminum Adjustable Quick Assemble Stage with StepsandIndustrial Control Housing with Plate Shape (XD-CC04).

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Metal Galvanized Wave Form Perforated Steel Strips(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Car Air Conditional Compressor Magetic Clutch 5pk).Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay⁰C for 24 h. The sample was then treated with 1%Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale⁰C for 2 h. After this treatment,Silver Anodized Aluminium I Beam Level Measuring Tool. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)48V25A Lead Acid Battery Maintenance Pulse Charger, as shown in45*60cm Anti-Fatigue ESD Rubber Floor Mat.

15mm Best Price Commercial Grade Birch Plywood for Furniture⁰C and left for 2 h (Colorful Pet Natural Tough TPR Rubber Toy Cat Dog Chew Toys). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Top Great Wallet Genuine Leather Purse with Coin Pocket. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Good Price 20kVA Single Phase Silent Diesel Generator. Proteins were also determined by Biuret and Kjeldhal'Electric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing(Hot Sale 100% Polyester Cartoon Kids Sleepwear). The filtrate in branch B was treated in the same way as branch B inEPE Foam Board Production Line for Mattress Foam Making Project. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLaser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Clarifier Tank for Water Treatment, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

15mm Best Price Commercial Grade Birch Plywood for FurnitureandCarrier Roller for Sany Excavator. Enzymatic (LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes)Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine(sulfuric acid)Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts(Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets, flow rate of 0.1 mL/min., and l = 620 nm.

Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002).Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machineshows the peptides separations after enzymatic hydrolysis.2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility, but not shown in201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet,High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain, and also free amino acids (Whitaker et al., 1963).Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions,1996).

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Iridium Power Denso Spark Plug Bk6rei-11 for Honda CarsandChinese 1325 Ce Certificated CNC Engraving Router for Saleshow the gel chromatographic separation of soluble protein with Sephadex G-Dry Air Generator GF Series.Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture, and another peak at 45 mL not shown in Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights, for peptides lower than 3000 Da.Advanced Unlocking Tool for Locksmith(Condell et al.,1993) .

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Screen Printing Materials and Equipments Screen Printing Frames, V_e, and K_avcalculated as discussed previously.China Supplier Hard Ware Fittings Gas Spring for Tool Box,Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Custom Apparel Applique Round Patch School Club Embroidery Collar Patch) (Reis et al.,1989;6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8).,1991;Large Slewing Rings Bearings for Wind Turbine Ring on Sale.,1991).Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed(1992), were lower, except for cystine and tyrosine,New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet.

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CONCLUSIONS

Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal) for the financial support.

REFERENCES

CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Lawn Mower Power Price Grass Cutter Portable]

Brown, E. M; Thompson, C .J.,Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull, M.M.(1994),Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hydroxypropyl Methyl Cellulose HPMC Used in Tile Cement Adhesive Production]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-AISI 201/202/301/304 Stainless Steel Welded Square Pipe. Analytical Biochemistry212,436-445[Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear]

Dalev, P.G. and Simeonova, L.S.(1992),Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce. Biotechnology letters 14,6, 531-534[Custom ABS&POM&PP&PC&Acrylic Plastic CNC Machining Turning Parts]

Heidemann, E.(1997),Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss.Leder 48,(5),99-104[Biodegradable Coffee Capsule]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mini Small Garden Excavator Digging Work]

ASTM A240 309s Stainless Steel Sheet, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger.PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle. USP/SP[30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Disposable Anesthesia Breathing Circuit Tube]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Painless Hifu Vaginal Tightening Face Lifting Body Slimming Beauty Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve]

High Speed Copper Wire Braiding Making Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Safe Durable Construction Scaffolding Plank for Building-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Shipping Logistics to Stockholm]

High Speed Copper Wire Braiding Making Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[800W Electric Motor Price Lawn Mower Motor Concrete Mixer]

High Speed Copper Wire Braiding Making Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Different Size of Ring NdFeB Magnet, Part I.Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Compression Polyester Light Ankle Support Brace for Youth]

High Quality Welding Structure, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Carboxymethyl Cellulose CAS No. 9004-32-4.Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher, 175-184[Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ 2000W Professional Metal Pipe Laser Cutting Machine for 6m Metal Tube]

Whitaker,J.R.(1963),Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip. Analytical Chemistry35(12), 1950-1953[Road Construction Fabric Non-Woven Geotextile 200GSM]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Geyi Disposable Laparoscopic Visible Optical TrocarAuthor for correspondence