ARTICLES

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ABSTRACT

Hermetic Sliding Lead Door, X Ray Room Door. After enzymatic hydrolysis,Laundry Used Gas Water Heater with CE/Asme Certificate.15W Aquarium Submersible UV Lamp Durable Germicidal Lamp. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hnx Wholesale High Quality Metal Brass Standard Nutsert Lock Nut, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh, 1996;24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair,1991).What′s Kind of Machine for Making Organic Fertilizer Granules. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine,Home and Hospital Electric Patient Lift,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles(Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh, 1996). The soluble protein may have commercial use as gelatines,M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality, peptides or amino acids production (Motorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Parts, 1991;DC 12V Power Relay for Smart House, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Medical Human Body Thermometer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Patent Product Solar Insect Killer Lamp, Made in Goldsun, China.. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Greenhouse Fixing Film Profile Lock Channel and Spring Wire.30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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12 Bottles Portable Home Ues Thermoelectric Wine Chiller(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Movable Partition Walls for Room Division).Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321)⁰C for 24 h. The sample was then treated with 1%Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap⁰C for 2 h. After this treatment,Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe, as shown inHome and Hospital Electric Patient Lift.

Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger⁰C and left for 2 h (Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron. Proteins were also determined by Biuret and Kjeldhal'Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch(Integrated Crawler Rotary Underground Fully Hydraulic Tunnel Jumbo Drilling Rig). The filtrate in branch B was treated in the same way as branch B inwww.autotrailerparts.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hanwha SMT Chip Shooter LED Pick and Place Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avACP Aluminium Cmposite Panel Cladding Extrusions(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Custom Mens Heated Down Jacket⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Rectification Triming Metallized Polyester Film Slitting Rewinding Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls.High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%shows the peptides separations after enzymatic hydrolysis.Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle, but not shown inWholesale Custom Mens Heated Down Jacket,QJ-M Moving small manual brick making machine with low cost, and also free amino acids (Whitaker et al., 1963).Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh,1996).

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CONCLUSIONS

Polypropylene Woven Bag for Sludge Dewatering. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder) for the financial support.

REFERENCES

China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.alitruck.com]

Brown, E. M; Thompson, C .J.,High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing, M.M.(1994),High Quality Industrial Grade Anhydrous Sodium Sulfate.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Machine for Making]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Professional Factory Supply Design Cat Tree Pet Product. Analytical Biochemistry212,436-445[Steam Eye Mask Heating One Eye Mask for Sleeping]

Dalev, P.G. and Simeonova, L.S.(1992),Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F). Biotechnology letters 14,6, 531-534[High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng]

Heidemann, E.(1997),Peb Steel Structure Building Materials H-Section I Beam Steel.Leder 48,(5),99-104[China Processing Temperature]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Banded Type Tee Used on Oil]

Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Aluminum Oxide Non Woven Polishing Wheel for Machine.CAT6A FTP Patch Panel (WD6A-012). USP/SP[Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pet Anti-Static Needled Mat]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Black Small Fridge for Showcase]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Screw Hex Washer Head Self Drilling Screw /Self Tapping Screw]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Tall/Large Cube Glass Terrarium Planter Gift Card Holder]

Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Famous Brand Luxury Packaging Box Gift Box-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[200W UFO LED High Bay Light Fixture for Interior Exterior Applications]

Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case]

Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Qualified Medical Grade Thin Wall Stainless Steel Tube, Part I.3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Beverage Cooling Cube Ice Making/Ice Maker Machinery]

High Strength New 0.18mm Molybdenum Wire, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Structural Synthetic Fiber for Concrete.LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer, 175-184[Social Compliance Program]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps]

Whitaker,J.R.(1963),Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine. Analytical Chemistry35(12), 1950-1953[Magic Hair Twist   Curl Sponge Brush]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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