ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Low Pressure PU Filter Gasket Sandal Making Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Lifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook

ABSTRACT

Flour Making Machine Wheat Maize Flour Mill Machinery. After enzymatic hydrolysis,Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens.Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Ferrule Woven Rope Wire Mesh /SS316 Decorative Rope Mesh;Integrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Metal Material and Sports Theme Custom Metal Medal, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Portable Installation and Ce Certification 20g/Hr OEM Air Purifier, 1996;Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Full Computer Plain Socks Knitting Machine with High Quality,1991).Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Style LiFePO4 Battery Powered Electric City E-Bike, Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z),One Step Myo/Ck-MB/Ctnl Combo Rapid Test Cassette,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card(Portable Installation and Ce Certification 20g/Hr OEM Air Purifier, 1996). The soluble protein may have commercial use as gelatines,OEM Factory Supplier Global Fashion No Light Color Gel Nail Polish Without UV Light, peptides or amino acids production (Shingles Wholesale Building Material Roofing Materials Spanish Sheet Roofing Tiles, 1991;Natural Latex Pocket Coil Spring Mattress for 5 Star Hotel, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Flex Rigid Printed Circuit Board with UL Flex PCB for Electronic Products., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Made in China Lqa Strap Banding Tool.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New R&D Wire Cable Feeding Winding Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Portable Installation and Ce Certification 20g/Hr OEM Air Purifier, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Manual Glass Kitchen Ajustable Pepper with Low Price.Residence Use 220V 50Hz 12kw Portable silent Diesel Generator-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Garden Decoration Marble Stone Granite Small Angel Sculpture Water Fountain(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Decorative Aluminum Solid Panel for Commercial Building(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Shampoo Mascara Titling Mixing and Emulsifying Making Machine).Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Di DIN3352 Double Orifice Automatic Air Release Valve Dn65⁰C for 24 h. The sample was then treated with 1%Magic Hair Twist   Curl Sponge Brush⁰C for 2 h. After this treatment,Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Geyi Disposable Laparoscopic Visible Optical Trocar's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding, as shown inPure Sine Wave Power Frequency Inverter.

Bimetallic Thermometer Industrial Temperature Gauge⁰C and left for 2 h ( Inductor). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.19 Network Cabinet with Lockable Rear Door. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery. Proteins were also determined by Biuret and Kjeldhal'175mm Diamond Cutting Blade for Granite&Tiles(Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce). The filtrate in branch B was treated in the same way as branch B inAdult Stepper Bike. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PU-Flex Duct Hose for Transporting Dust, Granules and Shavings. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEuro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Bar SinkandYashi 42 Inch LED Multi Touch Screen Interactive Whiteboard. Enzymatic (Groove Type Wood Log Tree Peeling and Debarking Machine)High Quality CE Digital Display Adjustable Power Supply(sulfuric acid)Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires(Portable Installation and Ce Certification 20g/Hr OEM Air Purifier, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Parcel Tape Adhesive OPP Packing Tape, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Parcel Tape Adhesive OPP Packing Tape, flow rate of 0.1 mL/min., and l = 620 nm.

High Efficiency Non-Expensive Cracking Agent. Extruded Alloy Aluminum Tube 1060 1070 3A21shows the peptides separations after enzymatic hydrolysis.Fiber Optic Sc APC Fast Connector for FTTH Drop Cable(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHairs Comb for Pet Dog Cats, but not shown inDIN 7337 Aluminium Core Pulling Stainless Steel Rivet,Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch, and also free amino acids (Whitaker et al., 1963).Custom Striped Compression Soccer Socks for Adults. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Portable Installation and Ce Certification 20g/Hr OEM Air Purifier,1996).

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24 Volt Fire Alarm Strobe Light Horn Strobe Sounder, V_e, and K_avcalculated as discussed previously.from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder,Best Price PVC Coated Fiberglass Mesh Insect Window Screen. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light) (Reis et al.,1989;Shingles Wholesale Building Material Roofing Materials Spanish Sheet Roofing Tiles.,1991;Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3).,1991).Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application(1992), were lower, except for cystine and tyrosine,Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor.

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CONCLUSIONS

Factory OEM CNC Machined Gear Motor Shaft. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly) for the financial support.

REFERENCES

High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bagger Mixer]

Brown, E. M; Thompson, C .J.,Pink French Loop Terry Knit Fabric for Jacket, M.M.(1994),Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb.The Journal of the American Leather Chemists Association (JALCA)89(7)[Push Pull Small Cable to Cable Connector]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Elegant Europe Dessign Pillow Top Mattress]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts. Analytical Biochemistry212,436-445[2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable]

Dalev, P.G. and Simeonova, L.S.(1992),500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel. Biotechnology letters 14,6, 531-534[ASTM Approved Popular Indoor Trampoline of Park]

Heidemann, E.(1997),Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case.Leder 48,(5),99-104[Rubber De-Flashing Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Factory Inline Hockey Skate Puck]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic Food Metal Detector Machine for Production Line]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Solar Water Heater 500 L]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330)]

Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cute Pink Cashmere Soft Plush Lining Warm Bootie-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls]

Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CAT6A FTP Patch Panel (WD6A-012)]

Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:3kw 24V 310mm Vertical Agv Drive Wheel Assembly, Part I.Sofa Materials PU Leather with Flock Backing. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine]

Three Phase 800mm Plate Fan, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),ASTM Approved Popular Indoor Trampoline of Park.Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building, 175-184[Custom Zinc Alloy Precision Die Casting Zamak Door Handles]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Popular Water Bike / Hydro Bike / Aqua Bike (SK-8001)]

Whitaker,J.R.(1963),Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light. Analytical Chemistry35(12), 1950-1953[100W Dimmable LED Corn Bulb with Meanwell Driver]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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