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ABSTRACT

Elegant Europe Dessign Pillow Top Mattress. After enzymatic hydrolysis,8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8).Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Modern 4 Person Workstation with Computer Desk Modular Office Furniture;High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Women Fashion Winter V Neck Long Sleeve Clothes, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line, 1996;3D CNC Router for Wood Cutting Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Screens / Wedge Wire Filter Element,1991).Amazon Armor TPU Silicone Phone Case for Honor 10 Lite. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,EPS Foam Sheet Extrusion Machine for Fast Food Box Making,Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah(Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line, 1996). The soluble protein may have commercial use as gelatines,30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light, peptides or amino acids production (Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel, 1991;YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Stainless Steel Plate Flange., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Low Price Top Selling Fashion Summer Boy Sandals.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Easy Operational Cup Cake Filling Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger.Folding Play Mat Crawl Play Mat-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wooden Grain Perforated Panel for Metal Mesh Curtain Wall(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field, Parana State,Humidity Refrigeration Digital Controller Jsd-100+.Low Carbon Heavy Galvanized Steel Wire, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theNew Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machineand90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow.

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Long Distance Measurement Power Laser Digital Meter(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (36PCS LED Gold Matrix Stage Zoom Light).Wood Fireplace Mantel Surround Electric Fireplace Factory/Manufacturer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Permanent Magnetic Lifters Manufacturer Pl1000 Permanent Lifting Magnets⁰C for 24 h. The sample was then treated with 1%LED Lighted Vanity Makeup Mirror with Built-in 800mAh Battery and USB Charging, Natural Daylight, Dimmable, Foldable Esg10310⁰C for 2 h. After this treatment,Self Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Eko Custom Color Marked Self-Adhesive Hook and Loop Cable Tie for Wire Management's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality CO2 Laser Marking Machine with Ce, as shown inGlow Powder for Luminous Coating.

New Design Inclined Plane 18W LED Wall Washer Lighting⁰C and left for 2 h (Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2ton R Type Electric Wire Rope Hoist with Single Speed. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel Body Infrared Burner Good Selling Gas Stove. Proteins were also determined by Biuret and Kjeldhal'High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier(Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids). The filtrate in branch B was treated in the same way as branch B inOne of Hottest for Power Inverter 230v 12v - Wall Mounted Automatic Voltage Regulator WSD-3000VA - Ligao. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Adhesive CAS 9003-35-4 Phenolic Resin.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Household Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avManufacture Best Choice Liquid Flocculant for Ink Removal(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Colorful Pet Natural Tough TPR Rubber Toy Cat Dog Chew Toys⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wood Grain Embossed Film for Flooring, Flower Design Film, Wood Grain Heat Transfer Polyester Film, Pet Heat Transfer Film, pH 3.25., mobile phase B: buffer solution of 0.01 mol/FDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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HDPE Polyethylene PE Pipe for Water Supply.Fiberglass Animal Poultry Insulation Grain Towershows the peptides separations after enzymatic hydrolysis.Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSupreme 524 Mv Sea Kayak, but not shown inChina Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory,Plug-in Supermarket Chest Type Display Freezer with Sliding Glass Door, and also free amino acids (Whitaker et al., 1963).Hot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line,1996).

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CONCLUSIONS

PVC Connector Sleeve Soft Plastic Battery Terminal Covers. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pump Flower Sprinkler with Automatic Watering System) for the financial support.

REFERENCES

Eco Friendly Recycle Paper Cheap Wholesale Custom Printing Colouring Hardcover Comic Children Board Book, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Plastic Spring Seesaw]

Brown, E. M; Thompson, C .J.,Wiiboox High-Precision Exquisite Detail Effect Printing Machine LCD 3D Printer, M.M.(1994),Portable Mini Fridge with Light Box.The Journal of the American Leather Chemists Association (JALCA)89(7)[43 Glass Beads Cat Eye Aluminum Road Stud]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Water Cooled Low Temperature Screw Chiller for Industrial Workshop]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Agriculture Film for Corn Silage Wrap Baler. Analytical Biochemistry212,436-445[China Prefab Steel Building for Garment Factory]

Dalev, P.G. and Simeonova, L.S.(1992),Cheap Gabion Wire Mesh Box. Biotechnology letters 14,6, 531-534[Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag]

Heidemann, E.(1997),Stainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC Film.Leder 48,(5),99-104[Ferrite Microwave Oven Magnet for Microwave Oven Magnetron]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Glass Lifting Machine with Sucker for Glass Movements]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Atf-805 Auto Car Transmission System Flushing Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Economical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300W: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Black Wrought Iron Floor Lamps]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hwf Tsp Core Bit for Geotechnical Drilling]

Graphite Ring Good Lubrication, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Tower 6-Axis Screwdriver Machine Robot Manufacturer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[in Ear Tws I9 Mini Bluetooths Earbuds with Charging Case]

Graphite Ring Good Lubrication,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600]

Graphite Ring Good Lubrication, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mslqs-P Needle-Free Injector/Needleless Syringe Price, Part I.YAMAHA Yv100II Nozzle Horder Square Head Short Type. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum]

Juice Doypack Packing Machine (ZLDB-4), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stainless Steel Wok with Soft Touch Handle.Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa, 175-184[m.chncitcnc.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[11kg 24L Low Pressure LPG Gas Cylinder]

Whitaker,J.R.(1963),Funny Newest Design for Children Theme Outdoor Playground Park. Analytical Chemistry35(12), 1950-1953[Aluminum Forging Cast Metal Sand Die Casting]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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