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ABSTRACT

High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck. After enzymatic hydrolysis,Aluminum Bright Polished Mirror Sheet.Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51, 1996;Customized Souvenirs Tin Metal Number & Name Plate for Car., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System,1991).Molasses Fuel Ethanol Distillation Plant. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Pond Self Priming Booster Pressure Water Pump with Check Valve,Banded Type Tee Used on Oil,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,20FT New Shipping Container 40FT Dry Cargo Container(Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51, 1996). The soluble protein may have commercial use as gelatines,High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover, peptides or amino acids production (Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone, 1991;High Pressure Braided Rubber Oil Hose, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Monochrome Graphic Industrial Control LCD Display Module 128X64., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.SMD LED Light-Operated USB Rechargeble Flashlight.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰1jo4 Hydraulic Adaptors for Hose Fittings.Chinese 1325 Ce Certificated CNC Engraving Router for Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Oil Heating Reactor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Molasses Fuel Ethanol Distillation Plant).Toilet Roll Tissue Wrapping Machine with Auto - Counting(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine⁰C for 24 h. The sample was then treated with 1%Orange Monofilament Fishing Tackle Nylon Fish Net⁰C for 2 h. After this treatment,Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)By6-8-200-L Automatic Doypack Premade Pouch Packing Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Diameter 1300mm Lifting Electro Magnet for Cast Iron, as shown inCentral Loose Tube No Armored 2 - 24 Core Fiber Optic Cable.

High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%⁰C and left for 2 h (SMD LED Light-Operated USB Rechargeble Flashlight). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Evaporator Finned Tube. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear. Proteins were also determined by Biuret and Kjeldhal'Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine(Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW)). The filtrate in branch B was treated in the same way as branch B inCable Tray And Cable Trunking. The final soluble protein shown were used for peptides separations using gel permeation chromatography,100%Polyester Spandex Hotel Chair Cover (DPH7022).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Factory Supply Perforated Metal Mesh. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCNC Turning Center, CNC Horizontal Turning Milling Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Classical Garment Accessories Jeans Button Metal Shank Button, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Vitamin D3 Fat-Solubility Water-Solubility Feed Additive, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Column Type Pave Rubber Tile Making Machine-Vulcanizing Press MachineandFactory Wholesale Zinc Plated Carbon Steel Hex Nut. Enzymatic (Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern)Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V(sulfuric acid)Stainless Steel Cheese Making Machine Electric(Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Plastic Injection Moulding Machine Supplier / Factory /Manufacturer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (All Kinds of Color Fashion Simple Stone Pendant Design Necklace) for the financial support.

REFERENCES

Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.songligroup.com]

Brown, E. M; Thompson, C .J.,15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable, M.M.(1994),Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure.The Journal of the American Leather Chemists Association (JALCA)89(7)[PP Needlepunched Nonwoven Geotextile]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-80W All in One Outdoor LED Street Light Solar Lamp. Analytical Biochemistry212,436-445[Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo)]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Sale Safety Vinyl PVC Flooring. Biotechnology letters 14,6, 531-534[Electrical Stationary Hoist Tower Crane 500kg Motor]

Heidemann, E.(1997),Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation.Leder 48,(5),99-104[Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Yashi Touch Screen Smart Interactive Whiteboard for Office]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fiber Optic To Bnc Converter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder]

Sanhe FC-2 Poultry Air Vent, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Picosecond Laser Tattoo Removal Skin Care Medical Instrument-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9]

Sanhe FC-2 Poultry Air Vent,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Lady Kids Child Yoga Clothes Gym Fitness Sports Wear]

Sanhe FC-2 Poultry Air Vent, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale, Part I.Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan]

Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),SUS316 Hexagon Socket Set Screws with Cone Point, DIN914.Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale, 175-184[Industrial Air Cooled Water Chiller Manufacturer Customized for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Different Size of Ring NdFeB Magnet]

Whitaker,J.R.(1963),Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor. Analytical Chemistry35(12), 1950-1953[High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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