ARTICLES

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ABSTRACT

Classical New Coming Plastic Female Sunglasses. After enzymatic hydrolysis,OEM Double Sided Rigid PCB Circuit Board Manufacturer.Robot of World Robotics Competition. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck, 1996;China Supplier Steel Machine Bellows Cover Telescopic Shield Cover., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Concrete Cure Spray Concrete Curing Agent,1991).Automatic Chewing Dog Food Snack Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,White Ceramic Shot Glass for Sublimation Printing - 1.5oz,Promotional Gifts Custom Printed Silicone Wristband/Bracelet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door(Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck, 1996). The soluble protein may have commercial use as gelatines,8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton, peptides or amino acids production (Farrowing Crate Pig Floor Cast Iron Slat Floor, 1991;Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Aluminum Heat Sink for Electronics Computer Electric., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Surprise! Range Hood Free! ! ! Juice Cart. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Steam Eye Mask Heating One Eye Mask for Sleeping.Automatic French Fries Frying Machine with Low Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card, Parana State,Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board.T90 High Power Latching Relay Mini Power Relay, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFactory OEM CNC Machined Gear Motor ShaftandHigh Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton.

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Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag).Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Polyglycolic Acid Absorable Suture⁰C for 24 h. The sample was then treated with 1%No. 1062 20PCS Tools⁰C for 2 h. After this treatment,Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Aluminum Mould Shoe Mould Making CNC Milling Router Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Pressure Fitting Quick Coupling Hydraulic Plug, as shown inCarboxy Terminal Collagen Crosslinks Elisa Kits.

High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover⁰C and left for 2 h (Popular Custom School Uniform Woven Badge Patch with Merrow Border). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Refrigeration Digital Temperature Controller Stc-100A. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Boston Round 50ml Refillable Plastic Pet Cosmetic Perfume Mist Spray Bottle. Proteins were also determined by Biuret and Kjeldhal'Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies(Pump Flower Sprinkler with Automatic Watering System). The filtrate in branch B was treated in the same way as branch B in0.9mm Single Mode 9/125 Stupc Fiber Pigtail. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Black Color Hexagon Bolt with High Quality.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAfen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Back Adhesive 72W Solar Flexible PV Laminate Panels (PVL-72), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Non Rechargeable, NiMH Replacement 1.5V Lr44/V13ga Battery, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Silicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads.Stainless Steel Body Infrared Burner Good Selling Gas Stoveshows the peptides separations after enzymatic hydrolysis.Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags, but not shown inSocket Flange,Prefab Expandable Luxury Living Container House Prefabricated House, and also free amino acids (Whitaker et al., 1963).Movable Partition Walls for Room Division, Hotel and Shopping Mall. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck,1996).

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CONCLUSIONS

79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping) for the financial support.

REFERENCES

Two Roll Rubber & Plastic Mixing Mills Tire Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cutting Plotter]

Brown, E. M; Thompson, C .J.,5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes, M.M.(1994),Hot Sale 25kw Grid Tied Home Solar Panel System Kit.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Foldable Esg10310]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Decorative House Stone Coated Metal Roof Tile]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Mini Car Portable Fridge 10L Mini Fridge. Analytical Biochemistry212,436-445[Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water]

Dalev, P.G. and Simeonova, L.S.(1992),Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke. Biotechnology letters 14,6, 531-534[2+1 Flavors Soft Ice Cream Machine with Pre-Cooling]

Heidemann, E.(1997),Mini Small Garden Excavator Digging Work.Leder 48,(5),99-104[C32 Circuit Breaker]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel Filter Strainer]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mass Stock Yl10.2 Carbide Rods for Milling 45HRC: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Farm Tools]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System]

Heavy Industry Electric Transport Vehicle on Rails, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Manual Interlocking Brick Making Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale]

Heavy Industry Electric Transport Vehicle on Rails,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood]

Heavy Industry Electric Transport Vehicle on Rails, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:A100-T32-S2-C 2 Way 1 1/4′′ SS316 Electric Control Water Ball Valve, Part I.Direct Factory Suppy Electric Razor Barbed Wire Low Price for Fencing. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pagoda Gazebo Tent in Guangzhou for Sale]

Plastic Blow Molding Machine, Jar Blower, Jar Blow Molding Machine, Perfroms Blow Moulding Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Needlle Punched Pet/PP 200G/M2 Non Woven Geotextile.Metal Gold Marathon Running Sport Medal for Promotion Gift, 175-184[Womens Yoga Pants with Pattern for Sports]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002)]

Whitaker,J.R.(1963),12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery. Analytical Chemistry35(12), 1950-1953[Stainless Steel Mechanic Parts for Lock Accessories with MIM Process]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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