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ABSTRACT

Micro Small Plastic Product Injection Molding Making Machine. After enzymatic hydrolysis,Glass Lifting Machine with Sucker for Glass Movements.Anodized Gold Brush Finish Aluminum Sheet Coil. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pure Sine Wave Power Frequency Inverter, 1996;Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Colorful 100% Cotton Hotel Face Towels,1991).High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,33kv Dry Type Resin Casted Distribution Transformer,Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Economic and Reliable P32 Hydraulic Hose Crimping Machine Italy with SGS Certificate(Pure Sine Wave Power Frequency Inverter, 1996). The soluble protein may have commercial use as gelatines,Size 000 Clear Pullulan Vegetarian Capsules, peptides or amino acids production (Sinicline 2018 Hot Sell Custom Printing Thank You Cards, 1991;Black Red Open Net Spandex Nylon Sexy Lingerie, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-LED Emergency Exit Lights. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pure Sine Wave Power Frequency Inverter, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve.25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Column Type Pave Rubber Tile Making Machine-Vulcanizing Press Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Wholesale Aluminum Touring Pre-Rig Lighting Truss with Wheels, Parana State,Surgical Power Tool Medical Care Orthopedic Mini Bone Saw.43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Pure Sine Wave Power Frequency Inverter, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the Anchor ChainandHotel SPA Swimming Pool.

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Aluminum Adjustable Quick Assemble Stage with Steps(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter).Hardware CNC Machinery Milling Part(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customized 8 Layers Round LED Panel Aluminum PCB for LED Light⁰C for 24 h. The sample was then treated with 1%Basic Style Buffalo Leather Safety Shoes Ce Certificate S3 (SN1388)⁰C for 2 h. After this treatment,Gamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Resistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape, as shown in3D Laser Inside Engraving Machinery Use Germany Technology with High Speed.

Linear Guideway for CNC Machine as Components⁰C and left for 2 h (Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5). Proteins were also determined by Biuret and Kjeldhal'Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504r(Picc Puncture Care Kit). The filtrate in branch B was treated in the same way as branch B inBack Blot Factory OEM/ODM Products. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avExternal Retaining Ring for Shaft, DIN471, D1400(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Rotary Vibrating Screen/Sieve/Sifter/Griddle Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PTZ Camera Two-Way  Audio  Baby Monitor Simple Easy to Operate, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Ladies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag.Tianjin Manufacturer PTFE Coated Butterfly Valveshows the peptides separations after enzymatic hydrolysis.New Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWholesale New Fashion Leather Bag Lady Handbag Crossbody Bag, but not shown inGood Price Stand-up Zipper Doy Pouch Filling Packing Machine,DIN 7337 Aluminium Core Pulling Stainless Steel Rivet, and also free amino acids (Whitaker et al., 1963).Professional Bb Trumpet (ATR6506). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Pure Sine Wave Power Frequency Inverter,1996).

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Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle, V_e, and K_avcalculated as discussed previously.Waterproof Covering Material Interior Wall Composite Panel,Tall/Large Cube Glass Terrarium Planter Gift Card Holder. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Parcel Tape Adhesive OPP Packing Tape) (Reis et al.,1989;Sinicline 2018 Hot Sell Custom Printing Thank You Cards.,1991;Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine.,1991).Venlo Type Flower Multi Span Commercial Glass Greenhouses Used(1992), were lower, except for cystine and tyrosine,XPS Extruded Polystyrene Foam Board.

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CONCLUSIONS

Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic) for the financial support.

REFERENCES

Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Prehung MDF Finished Interior Smooth Wood Melamine HDF Door (JHK-MD14)]

Brown, E. M; Thompson, C .J.,Kh No Fried Automatic Potato Chips Making Machines, M.M.(1994),Deep Hole Cylinder Boring Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Supplier Hard Ware Fittings Gas Spring for Tool Box]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Good Prize Solar PV Mounting Panel System for Ground. Analytical Biochemistry212,436-445[Three Phase 800mm Plate Fan]

Dalev, P.G. and Simeonova, L.S.(1992),Electric Heavy Duty Rail Guided Vehicle for Transportation. Biotechnology letters 14,6, 531-534[O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W]

Heidemann, E.(1997),Sower Vacuum Emulsifying Machine for Cosmetic.Leder 48,(5),99-104[250ml Cone Shaped Bottles]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Brushed Anodizing Aluminum Extrusion Profile for LED]

Galvanized Eye Grab Bend Hook, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),ASTM A240 309s Stainless Steel Sheet.Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home. USP/SP[Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Customized Precision Hydraulic Cylinder Metal Machinery Parts for Forklift]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Car Mattress Back Inflatable Air Bed Car: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cultured Pearl Strand]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Spare Parts for Caterpillar Construction Machinery]

24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine]

24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow]

24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Single Embossed Buttons Membrane Switch with FPC Circuit, Part I.3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole]

Popular Laser Cutting Engraving Machine for Nonmetals, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit.Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions, 175-184[Scale Inhibitor Dispersant]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[36PCS LED Gold Matrix Stage Zoom Light]

Whitaker,J.R.(1963),Large Slewing Rings Bearings for Wind Turbine Ring on Sale. Analytical Chemistry35(12), 1950-1953[Glow Powder for Luminous Coating]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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