ARTICLES

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ABSTRACT

Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable. After enzymatic hydrolysis,Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars.Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Freshwater Pearl Butterfly Flower Bride Hairpin;Air Bubble Insulation Thermal Building Insulation Material; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Rechargeable Battery Operated Cooling Folding Electric Fan, 1996;High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (21.5′′ Wall Mount Display Capacitive Touch Monitor,1991).Pagoda Gazebo Tent in Guangzhou for Sale. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe,Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Black Small Fridge for Showcase(Rechargeable Battery Operated Cooling Folding Electric Fan, 1996). The soluble protein may have commercial use as gelatines,Microwave Motion Sensor Module Doppler Radar Detector (HW-M10), peptides or amino acids production (Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile, 1991;Zircon Butterfly Open Ring Diamond Fashion Woemn Rings, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Spare Parts Custom Injection Plastic Moulding Manufacturer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Rechargeable Battery Operated Cooling Folding Electric Fan, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood.Kingdomway Brand Vitamin a 1000-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

45*60cm Anti-Fatigue ESD Rubber Floor Mat, Parana State,OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part.Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Rechargeable Battery Operated Cooling Folding Electric Fan, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFM USB Radio Audio Power Amplifier for Home Bar ClubandYashi Touch Screen Smart Interactive Whiteboard for Office.

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Vinyl Glove in Work & Labor Glove(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder).Low Energy Needed Compact Flour Mill Plant for Milling Wheat(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Nylon Weatherproof PA6 Strain Relief Cable Gland⁰C for 24 h. The sample was then treated with 1%Steel Roller Embossed Paper 2019 New Design⁰C for 2 h. After this treatment,Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Factory Price DIN5685 Galvanized Marine Anchor Link Chain's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001), as shown inNew Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9.

6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck⁰C and left for 2 h (Lady Kids Child Yoga Clothes Gym Fitness Sports Wear). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Level Application Waterproof Plastic Box. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine. Proteins were also determined by Biuret and Kjeldhal'Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse(Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan). The filtrate in branch B was treated in the same way as branch B inYogo cloth. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Water Slide for Indoor Play Structure.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av1220*2440mm Concrete Formwork black film faced plywood(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/SHXJ1500 CNC Diamond Wire Stone Cutting Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Different Size of Ring NdFeB MagnetandMen′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag. Enzymatic (2019 Foam Concrete Machine/Foamed Concrete Block Making Machine)Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball(sulfuric acid)Yyh Series One Dimension Tumbling Mixer Mixing Machine(Rechargeable Battery Operated Cooling Folding Electric Fan, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%ODU 14 Pin Industrial Circular Connector, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%ODU 14 Pin Industrial Circular Connector, flow rate of 0.1 mL/min., and l = 620 nm.

Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board.High Crush Strength Activated Alumina as Petrochemical Catalyst Carriershows the peptides separations after enzymatic hydrolysis.Classical New Coming Plastic Female Sunglasses(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSlewing Gear for Tower Crane Slewing Winch Reducer, but not shown inLPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer,OEM Double Sided Rigid PCB Circuit Board Manufacturer, and also free amino acids (Whitaker et al., 1963).Robot of World Robotics Competition. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Rechargeable Battery Operated Cooling Folding Electric Fan,1996).

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Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck, V_e, and K_avcalculated as discussed previously.China Supplier Steel Machine Bellows Cover Telescopic Shield Cover,Concrete Cure Spray Concrete Curing Agent. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag) (Reis et al.,1989;Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile.,1991;High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve.,1991).Automatic Chewing Dog Food Snack Machine(1992), were lower, except for cystine and tyrosine,White Ceramic Shot Glass for Sublimation Printing - 1.5oz.

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CONCLUSIONS

Promotional Gifts Custom Printed Silicone Wristband/Bracelet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale Black Galvanized Head Stainless Steel Screw) for the financial support.

REFERENCES

Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cast Iron Skillet/Fry Pan 13.25]

Brown, E. M; Thompson, C .J.,8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton, M.M.(1994),Farrowing Crate Pig Floor Cast Iron Slat Floor.The Journal of the American Leather Chemists Association (JALCA)89(7)[Steel Forged Automobile Spare Parts with Customized Drawing]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing. Analytical Biochemistry212,436-445[ 100p]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Aluminum Heat Sink for Electronics Computer Electric. Biotechnology letters 14,6, 531-534[Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory]

Heidemann, E.(1997),5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover.Leder 48,(5),99-104[E91T1-B3C Low Alloy Steel Flux Cored Wire]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale]

Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Surprise! Range Hood Free! ! ! Juice Cart.Steam Eye Mask Heating One Eye Mask for Sleeping. USP/SP[New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Economical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300W]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automatic French Fries Frying Machine with Low Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[8oz 250ml Disposable Paper Soup Bowl with Lid for Take Away]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tower Crane Load Moment Limit Switch for Safety Operation]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna]

Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring]

Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap]

Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security, Part I.Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[CNC Machining Non-Standard Hardware Parts]

Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card.T90 High Power Latching Relay Mini Power Relay, 175-184[www.growook.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce]

Whitaker,J.R.(1963),Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch. Analytical Chemistry35(12), 1950-1953[Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Fast Supplier Juice Sealing Film Wrapping Packing Machine Production LineAuthor for correspondence