ARTICLES

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ABSTRACT

Garden Decoration Marble Stone Granite Small Angel Sculpture Water Fountain. After enzymatic hydrolysis,530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle.PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Carbon Graphite Crucible, 1996;Decorative Aluminum Solid Panel for Commercial Building., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing,1991).Di DIN3352 Double Orifice Automatic Air Release Valve Dn65. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Magic Hair Twist   Curl Sponge Brush,Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Geyi Disposable Laparoscopic Visible Optical Trocar(Carbon Graphite Crucible, 1996). The soluble protein may have commercial use as gelatines,Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding, peptides or amino acids production (Bimetallic Thermometer Industrial Temperature Gauge, 1991;19 Network Cabinet with Lockable Rear Door, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.175mm Diamond Cutting Blade for Granite&Tiles.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Carbon Graphite Crucible, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PU-Flex Duct Hose for Transporting Dust, Granules and Shavings.Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Uprigth Touch-Screen Red Wine Chiller(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Eyes- Caring LED Night Light for Nursing Reading with Soft Light).Parcel Tape Adhesive OPP Packing Tape(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Efficiency Non-Expensive Cracking Agent⁰C for 24 h. The sample was then treated with 1%Fiber Optic Sc APC Fast Connector for FTTH Drop Cable⁰C for 2 h. After this treatment,Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Custom Striped Compression Soccer Socks for Adults's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738), as shown in24 Volt Fire Alarm Strobe Light Horn Strobe Sounder.

Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen⁰C and left for 2 h (YAMAHA Yv100II Nozzle Horder Square Head Short Type). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Tensile Strength Galvanized Steel Wire. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,24 Volt Fire Alarm Strobe Light Horn Strobe Sounder. Proteins were also determined by Biuret and Kjeldhal'from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder(Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel). The filtrate in branch B was treated in the same way as branch B inFood Grade High Borosilicate Sealed Glass Jar with Frosted Lid. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Best Price PVC Coated Fiberglass Mesh Insect Window Screen.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBest Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Pink French Loop Terry Knit Fabric for Jacket, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Corrugated Board Production LineandDie Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts. Enzymatic (500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel)Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case(sulfuric acid)Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600)(Carbon Graphite Crucible, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall, flow rate of 0.1 mL/min., and l = 620 nm.

Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit.T90 High Power Latching Relay Mini Power Relayshows the peptides separations after enzymatic hydrolysis.Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLuxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe, but not shown inSafe Durable Construction Scaffolding Plank for Building,Cute Pink Cashmere Soft Plush Lining Warm Bootie, and also free amino acids (Whitaker et al., 1963).3kw 24V 310mm Vertical Agv Drive Wheel Assembly. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Carbon Graphite Crucible,1996).

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CONCLUSIONS

OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery) for the financial support.

REFERENCES

Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[500gsm Laser Cut Coral Fleece Water Absorption Towel 500gsm Wholesaler Quotes & PriceList]

Brown, E. M; Thompson, C .J.,Partial Thread Steel/Iron Hex Head Self Tapping Screw, M.M.(1994),Flat Car Sun Roof Tent Awning.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Original Testo 174t Mini Temperature Data Logger No. 0572 1560]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Classic Black Color Commercial Glass Door Multi Deck Refrigerator. Analytical Biochemistry212,436-445[Vitamin D3 Fat-Solubility Water-Solubility Feed Additive]

Dalev, P.G. and Simeonova, L.S.(1992),Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits. Biotechnology letters 14,6, 531-534[Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card]

Heidemann, E.(1997),12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026).Leder 48,(5),99-104[7005 7050 7075 Alloy Extruded Aluminum Rectangular Tube]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric]

New R&D Wire Cable Feeding Winding Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Pneumatic Control Shuttle Valve NPT Thread.Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing. USP/SP[Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM 15PCS Electric Face Makeup Brush with Ce Certificate: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fast Delivery Time Supermarket Island Refrigerator Round Open Display Chiller]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[100W 120W 150W Outdoor Solar LED Street Lighting]

New Styles Fashion Erotic Women Underwear Sexy Lingerie, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[PAC /Poly Aluminium Chloride for Water Treatment]

New Styles Fashion Erotic Women Underwear Sexy Lingerie,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Galvanized Roller Shutter Window Torsion Springs]

New Styles Fashion Erotic Women Underwear Sexy Lingerie, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stand Hot and Cold Normal Water Dispenser with Refrigerator, Part I.High Quality Construction Welding Rods Electrode E6013 E7018 E7016. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Italy Design Good Quality Hydraulic Motorcycle Lift Table]

High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular.Lydite Fence Reel Reel with Extension Arms for Farm Fencing, 175-184[250 Kw 100 HP Industrial Air Cooled Screw Water Chiller]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck]

Whitaker,J.R.(1963),1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio. Analytical Chemistry35(12), 1950-1953[Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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