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ABSTRACT

Factory Direct Sale Single Cloth Hanger Hook. After enzymatic hydrolysis,2 Round Sash Pulley for Chain - Brass (PVD).Anodized Aluminum Expanded Metal Mesh Competitive Price. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Round Shape Aluminum PCB and PCBA, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (36PCS LED Gold Matrix Stage Zoom Light, 1996;Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Chicken Cage Mesh Hexagonal Wire Mesh Machine,1991).110/220V Handle Mild Steel Welding Machine PCB Board 160A. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming,Enamel Cast Iron Mini Casserole Cast Iron Cocotte,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part(36PCS LED Gold Matrix Stage Zoom Light, 1996). The soluble protein may have commercial use as gelatines,Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer, peptides or amino acids production (Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug, 1991;Automatic Cashew Processing Cashew Nuts Shelling Peeling Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;CNC System Air Duct Line5 Make Machine for HVAC., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Titanium Dioxide Anatase for Paper Making.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-40A C Electronic Air Circuit Breaker with Dz47-4p. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (36PCS LED Gold Matrix Stage Zoom Light, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Pipe Thermostat with Sensor Ce Certificate.Walking Long Distance Surveying Instrument Meter Measuring Wheel-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Chinese Supplier of PVC Profile(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPositive Drive Turn Belt for Spiral Freezing Equipment(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.S Series China Generator Helical-Worm Crushers Reduction Box⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Original Testo 174t Mini Temperature Data Logger No. 0572 1560, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CNC Machining Non-Standard Hardware Parts, V_e, and K_avcalculated as discussed previously.DOT Aluminum Beverage CO2 Gas Cylinder 5lb 10lb 15lb 20lb,Galvanized Solar System Mounting Bracket Connector. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (355nm UV Laser Marking Equipment for Crystal Materials) (Reis et al.,1989;Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug.,1991;Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China.,1991).Digital Programmable Ntc Temperature Controller(1992), were lower, except for cystine and tyrosine,Water Meter Spare Parts Rubber Rings for Sealing.

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CONCLUSIONS

Stainless Steel HK Series Needle Roller Bearing HK0408. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale Prices of 20W 40W 60W Solar Street Lights) for the financial support.

REFERENCES

Road Construction Fabric Non-Woven Geotextile 200GSM, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Non-Biodegradable Bamboo Fiber Bread/Lunch Box (YK-B3072)]

Brown, E. M; Thompson, C .J.,High Polymer Modified Cement Based Elastic Waterproof Coating, M.M.(1994),Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe.The Journal of the American Leather Chemists Association (JALCA)89(7)[Plastic Caps Making Machine / Injection Molding Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Factory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chiller]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset. Analytical Biochemistry212,436-445[Loquat Juice and Loquat Syrup Production Machinery Export Worldwide]

Dalev, P.G. and Simeonova, L.S.(1992),Fashion Shoulder Backpack Handbag School Bag. Biotechnology letters 14,6, 531-534[12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Reasonable Price High Quality Nylon Cable Gland]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Folding Round Picnic Caravan Table RV Picnic Table Camping Table]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Speed Cable Wire Winding Braiding Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Walking Long Distance Surveying Instrument Meter Measuring Wheel]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Exquisite Small Glass Bird′s Nest Bottle Manufacturers]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Micro DC 24V Worm Gear Brushless Motor for Medical Equipment]

22 High Rubber Mixing Mill, High Accuracy Lab Rubber Two Roll Mill, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Temperature Alternative Stainless Steel Band in Different Package-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Carboxymethyl Cellulose CAS No. 9004-32-4]

22 High Rubber Mixing Mill, High Accuracy Lab Rubber Two Roll Mill,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle]

22 High Rubber Mixing Mill, High Accuracy Lab Rubber Two Roll Mill, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Water Cooled Low Temperature Screw Chiller for Industrial Workshop, Part I.Home Machine 2019 New Commercial Treadmill Gym Equipment. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Buy Mk 677 Powder Bulk Powders Sarms Mk 677]

Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Residential Reverse Osmosis 0.0001um Water Filter Water Filtration.Rg178 Coaxial Cable with Ipex/Ipx Female to SMA Male Right Angle, 175-184[Stainless Steel Ss Chocolate Melting Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5)]

Whitaker,J.R.(1963),China Cheap Polarized Sunglasses Hot Sale Metal Sunglasses. Analytical Chemistry35(12), 1950-1953[OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back CoverAuthor for correspondence