ARTICLES

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ABSTRACT

Gamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge. After enzymatic hydrolysis,Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3).Resistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Linear Guideway for CNC Machine as Components;Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504r, 1996;Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W,1991).External Retaining Ring for Shaft, DIN471, D1400. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber,Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Rotary Vibrating Screen/Sieve/Sifter/Griddle Machine(Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504r, 1996). The soluble protein may have commercial use as gelatines,PTZ Camera Two-Way  Audio  Baby Monitor Simple Easy to Operate, peptides or amino acids production (Custom Rubber Product Seal O-Ring /NBR70 O Ring, 1991;Fully Automatic Dust Face Mask Making Machine with Ultrasonic, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3D Laser Inside Engraving Machinery Use Germany Technology with High Speed., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-460ml Glassware/Wine /Alcohol/Spirits/Glass Vodka Bottle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504r, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ladies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag.New Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:DIN 7337 Aluminium Core Pulling Stainless Steel Rivet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle, Parana State,Basic Style Buffalo Leather Safety Shoes Ce Certificate S3 (SN1388).Waterproof Covering Material Interior Wall Composite Panel, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504r, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOutdoor Garden Save Energy with Solar Flame Wall LightandDaily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder.

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Tall/Large Cube Glass Terrarium Planter Gift Card Holder(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver).Venlo Type Flower Multi Span Commercial Glass Greenhouses Used(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%XPS Extruded Polystyrene Foam Board⁰C for 24 h. The sample was then treated with 1%Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture⁰C for 2 h. After this treatment,Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Kh No Fried Automatic Potato Chips Making Machines's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Deep Hole Cylinder Boring Machine, as shown in860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag.

Good Prize Solar PV Mounting Panel System for Ground⁰C and left for 2 h (Natural Latex Pocket Coil Spring Mattress for 5 Star Hotel). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Electric Heavy Duty Rail Guided Vehicle for Transportation. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Sower Vacuum Emulsifying Machine for Cosmetic. Proteins were also determined by Biuret and Kjeldhal'ASTM A240 309s Stainless Steel Sheet(Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h). The filtrate in branch B was treated in the same way as branch B inSurvival Water Filter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Car Mattress Back Inflatable Air Bed Car. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Single Embossed Buttons Membrane Switch with FPC Circuit⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Popular Laser Cutting Engraving Machine for Nonmetals, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Steel Forged Automobile Spare Parts with Customized DrawingandDisposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit. Enzymatic (Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions)Large Slewing Rings Bearings for Wind Turbine Ring on Sale(sulfuric acid)Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container(Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504r, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Chemicals Flocculant Cationic Polymer Price, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Chemicals Flocculant Cationic Polymer Price, flow rate of 0.1 mL/min., and l = 620 nm.

Ycom USB Charger and Data Cable with Aluminum for Type C.Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machineshows the peptides separations after enzymatic hydrolysis.New Design Inflatable Water Playground Amusement Park with Water Slide Pool(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in 100p, but not shown inStainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory,240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface, and also free amino acids (Whitaker et al., 1963).Light Weight Polyester Eye Mask for Travel Airplane Hotel. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504r,1996).

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CONCLUSIONS

Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3.5ton Chinese Engine Gasoline Forklift (FG35T)) for the financial support.

REFERENCES

Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[UV Resistance]

Brown, E. M; Thompson, C .J.,PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal, M.M.(1994),500 Tons Hydraulic Power Double Action Press with Cushion.The Journal of the American Leather Chemists Association (JALCA)89(7)[Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine. Analytical Biochemistry212,436-445[Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line]

Dalev, P.G. and Simeonova, L.S.(1992),Promotional Plastic Laminated Custom Shopping PP Woven Bag. Biotechnology letters 14,6, 531-534[Southeast Asia Healthy Soft Integrated Latex Foam Mattress]

Heidemann, E.(1997),Metal Galvanized Wave Form Perforated Steel Strips.Leder 48,(5),99-104[Studio and Theater]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Classical New Coming Plastic Female Sunglasses]

Hardware CNC Machinery Milling Part, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12).Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay. USP/SP[Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Rotary Vibrating Screen/Sieve/Sifter/Griddle Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Thermal Cycle Test Equipment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit]

Silver Anodized Aluminium I Beam Level Measuring Tool, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Cheap Polarized Sunglasses Hot Sale Metal Sunglasses]

Silver Anodized Aluminium I Beam Level Measuring Tool,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Children Educational Toys Game]

Silver Anodized Aluminium I Beam Level Measuring Tool, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:48V25A Lead Acid Battery Maintenance Pulse Charger, Part I.15mm Best Price Commercial Grade Birch Plywood for Furniture. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[L Shape U Shape Big Corner Home Villa Living Room Hotel Lobby Full Chinese Italy Leather with Manual Movable Headrest Confortable Sofa Set Furniture]

Top Great Wallet Genuine Leather Purse with Coin Pocket, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Good Price 20kVA Single Phase Silent Diesel Generator.Electric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing, 175-184[Cross Motorcycle Tire]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Bearing with Housing Bearing / Pillow Block Bearing]

Whitaker,J.R.(1963),Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals. Analytical Chemistry35(12), 1950-1953[Milling Precision Stainless Steel CNC Machinery Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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