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ABSTRACT

30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products. After enzymatic hydrolysis,Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle.RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads, Heavy Blanket, Gravity Blanket, Weighted Blanket;Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Supplier Granular Coal Based Activated Carbon Filter Meida, 1996;Glass Cutting Table/CNC Glass Cutting Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (12 Bottles Portable Home Ues Thermoelectric Wine Chiller,1991).Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321),Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening(China Supplier Granular Coal Based Activated Carbon Filter Meida, 1996). The soluble protein may have commercial use as gelatines,Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle, peptides or amino acids production (Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe, 1991;Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Supplier Granular Coal Based Activated Carbon Filter Meida, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt.Hanwha SMT Chip Shooter LED Pick and Place Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:ACP Aluminium Cmposite Panel Cladding Extrusions(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making MachineAlcalase2.5 L DX,Wholesale Custom Mens Heated Down Jacket, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Rectification Triming Metallized Polyester Film Slitting Rewinding Machine); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

New Swim Shorts Grey Mens Board Shorts with Mesh Liner, Parana State,Greenhouse Fixing Film Profile Lock Channel and Spring Wire.Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China Supplier Granular Coal Based Activated Carbon Filter Meida, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChinese Galvanized Rectangular Tube for Buildings MaterialsandUF Membrane Aqu160 with Competitive Price and High Quality.

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Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factory Price DIN5685 Galvanized Marine Anchor Link Chain).Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Marine Mooring Hardware, Anchor Chain⁰C for 24 h. The sample was then treated with 1%Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls⁰C for 2 h. After this treatment,Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)QJ-M Moving small manual brick making machine with low cost's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device, as shown inVertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C).

Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set⁰C and left for 2 h (China Supplier Steel Machine Bellows Cover Telescopic Shield Cover). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Braiding Machine for Weaving Flexible Metal Hose. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking. Proteins were also determined by Biuret and Kjeldhal'Fashion Metal Button Crystal Shank Button for Garment(Factory Supply Perforated Metal Mesh). The filtrate in branch B was treated in the same way as branch B inYL10.2 Tungsten Carbide Rod Blank for Endmills and Drill Bits. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIndustrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Polypropylene Woven Bag for Sludge Dewatering⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Flexible Polyurethane Tube PU Spring Coil Hose Pneumatic Colorful Spiral HoseandHigh Quality Industrial Grade Anhydrous Sodium Sulfate. Enzymatic (Professional Factory Supply Design Cat Tree Pet Product)Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F)(sulfuric acid)Peb Steel Structure Building Materials H-Section I Beam Steel(China Supplier Granular Coal Based Activated Carbon Filter Meida, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Aluminum Oxide Non Woven Polishing Wheel for Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Aluminum Oxide Non Woven Polishing Wheel for Machine, flow rate of 0.1 mL/min., and l = 620 nm.

CAT6A FTP Patch Panel (WD6A-012).Widely Application Latest Designed Coffee Capsule Filling Packing Machineshows the peptides separations after enzymatic hydrolysis.LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNeedlle Punched Pet/PP 200G/M2 Non Woven Geotextile, but not shown inPotassium Hydroxide (KOH) Flakes 90% Purity Best Price Qingdao Hiseachem,Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant, and also free amino acids (Whitaker et al., 1963).Famous Brand Luxury Packaging Box Gift Box. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China Supplier Granular Coal Based Activated Carbon Filter Meida,1996).

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Ss Reduced Port 2000wog 1PC Screwed Ball ValveandResidential Reverse Osmosis 0.0001um Water Filter Water Filtrationshow the gel chromatographic separation of soluble protein with Sephadex G-High Qualified Medical Grade Thin Wall Stainless Steel Tube.3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry, and another peak at 45 mL not shown in1jo4 Hydraulic Adaptors for Hose Fittings, for peptides lower than 3000 Da.High Strength New 0.18mm Molybdenum Wire(Condell et al.,1993) .

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Structural Synthetic Fiber for Concrete, V_e, and K_avcalculated as discussed previously.LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer,Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low) (Reis et al.,1989;Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe.,1991;Glass Cutting Table/CNC Glass Cutting Machine.,1991).Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine(1992), were lower, except for cystine and tyrosine,Tct Circular Saw Blade for Grass Cutting.

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CONCLUSIONS

Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Rectification Triming Metallized Polyester Film Slitting Rewinding Machine) for the financial support.

REFERENCES

Picc Puncture Care Kit, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Customized Aluminium Foil Mailer Bubble Envelope Express Mailing Bag in Metallic Color]

Brown, E. M; Thompson, C .J.,Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth, M.M.(1994),AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture.The Journal of the American Leather Chemists Association (JALCA)89(7)[Plastic Injection Moulding Machine Supplier / Factory /Manufacturer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-34*25cm Office File Pocket Felt A4 Document Bag. Analytical Biochemistry212,436-445[Whole Set Gas Spring Strut for Furniture]

Dalev, P.G. and Simeonova, L.S.(1992),PP Needlepunched Nonwoven Geotextile. Biotechnology letters 14,6, 531-534[Union Metal Steel Building and Construction Material]

Heidemann, E.(1997),High Temperaturethermocouple Male and Female Plug.Leder 48,(5),99-104[Llw Screen Filtration Centrifuge]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Factory Direct Sale Single Cloth Hanger Hook]

Medical Human Body Thermometer, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts.Best Price Custom Embroidery Logo Hotel Slippers. USP/SP[27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Band Resaw for Lumber]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Si3n4 Full Ceramic  Bearing  608 Size 8X22X7]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[30 Ppi Foam Filter Sheet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Qualified Medical Grade Thin Wall Stainless Steel Tube]

High Efficiency Fine Powder Vibrating Screener, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fruit Plum Cleaning Washing Machine with Brushes Spray]

High Efficiency Fine Powder Vibrating Screener,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage]

High Efficiency Fine Powder Vibrating Screener, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Plant Stands & Accessories Glass Hanging Flower Pot Bracket, Part I.Wooden Frame Stretched Canvas, Painting Canvas. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Promotional Plastic Laminated Custom Shopping PP Woven Bag]

Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF.UF Membrane Aqu160 with Competitive Price and High Quality, 175-184[Cement Brick Making Machine Curbstone Block Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[OEM/ODM Custom Made Muslim Bridal Wedding Gown Dress]

Whitaker,J.R.(1963),Atf-805 Auto Car Transmission System Flushing Machine. Analytical Chemistry35(12), 1950-1953[ Granules and Shavings]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether SulfateAuthor for correspondence