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ABSTRACT

Tube Mills for Thin Wall Tubing. After enzymatic hydrolysis,Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core.Car Badge Custom Emblem Auto Logo Sign for Advertising. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Rear Wheel Exchangeable Aluminum Manual Wheelchair, 1996;90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Graphic 128X128 Dots Cog LCD,1991).Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Tcn Sex Toy Vending Machines for Sale S800-10,Silver 8 Inch Double Thick Round Cake Board,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Precision Machining Auto Parts Metal Contact(Rear Wheel Exchangeable Aluminum Manual Wheelchair, 1996). The soluble protein may have commercial use as gelatines,Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label, peptides or amino acids production (Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler, 1991;Paper Sticky Lint Remover Roller/Pet Hair Roller, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Safe Durable Scaffolding Trapdoor Plank for Construction., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Various Sublimation Blank Home Supply Fabric Bath Towel etc. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Rear Wheel Exchangeable Aluminum Manual Wheelchair, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic.Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEyes- Caring LED Night Light for Nursing Reading with Soft Light(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/The Most Competitive Sodium Gluconate for Water Reducer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Milling Precision Stainless Steel CNC Machinery Parts) for the financial support.

REFERENCES

Popular Custom School Uniform Woven Badge Patch with Merrow Border, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ceramic Jars With Wood Lids]

Brown, E. M; Thompson, C .J.,1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor, M.M.(1994),Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter.The Journal of the American Leather Chemists Association (JALCA)89(7)[Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Electrical Stationary Hoist Tower Crane 500kg Motor. Analytical Biochemistry212,436-445[Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant]

Dalev, P.G. and Simeonova, L.S.(1992),Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage. Biotechnology letters 14,6, 531-534[Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull]

Heidemann, E.(1997),Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange.Leder 48,(5),99-104[Copper Wire Coiled Wire Spiral Cable Wire Double Shielded Power Cable]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ADC12 Aluminum Die Casting Parts]

Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature.Flow Wrapper/ Bakery Moon Cake Packing Machine. USP/SP[Safest Baby Healthcare and Grooming Kit for Baby Gift]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Original Testo 174t Mini Temperature Data Logger No. 0572 1560]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Back Color Vertical Double Sucker with Plastic Handle]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Vitamin D3 Fat-Solubility Water-Solubility Feed Additive]

Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Reusable Metal Stainless Steel Drinking Straws-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card]

Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric]

Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Designed Steel Frame Factory Building, Part I.Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture]

Whitaker,J.R.(1963),Glass Mix Stone Mosaic Tiles. Analytical Chemistry35(12), 1950-1953[Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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