ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High Speed Roll Plastic T-Shirt Bag Making Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine

ABSTRACT

Wholesale Black Galvanized Head Stainless Steel Screw. After enzymatic hydrolysis,PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine.45*60cm Anti-Fatigue ESD Rubber Floor Mat. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints;Vinyl Glove in Work & Labor Glove; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Low Energy Needed Compact Flour Mill Plant for Milling Wheat, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Nylon Weatherproof PA6 Strain Relief Cable Gland, 1996;Steel Roller Embossed Paper 2019 New Design., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil,1991).Factory Price DIN5685 Galvanized Marine Anchor Link Chain. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001),6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Level Application Waterproof Plastic Box(Nylon Weatherproof PA6 Strain Relief Cable Gland, 1996). The soluble protein may have commercial use as gelatines,Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine, peptides or amino acids production (Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse, 1991;Water Slide for Indoor Play Structure, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.1220*2440mm Concrete Formwork black film faced plywood.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Washami Teeth Care for Whitening Cool Mint Toothpaste. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Nylon Weatherproof PA6 Strain Relief Cable Gland, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope.Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:SHXJ1500 CNC Diamond Wire Stone Cutting Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

ODU 14 Pin Industrial Circular Connector, Parana State,Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine.Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Nylon Weatherproof PA6 Strain Relief Cable Gland, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theClearing Clogged Effectively Pipe Drain Cleaning Machine (A75)andGym Fitness Equipment Adjustable Weight Bench.

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Classical New Coming Plastic Female Sunglasses(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card).OEM Double Sided Rigid PCB Circuit Board Manufacturer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Robot of World Robotics Competition⁰C for 24 h. The sample was then treated with 1%Professional Rope/Cable/Wire Braiding Machine with Factory Price⁰C for 2 h. After this treatment,Aluminum PCB Circuit Board SMD PCBA LED PCB. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck, as shown inReusable Metal Stainless Steel Drinking Straws.

China Supplier Steel Machine Bellows Cover Telescopic Shield Cover⁰C and left for 2 h (Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Concrete Cure Spray Concrete Curing Agent. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Automatic Chewing Dog Food Snack Machine. Proteins were also determined by Biuret and Kjeldhal'White Ceramic Shot Glass for Sublimation Printing - 1.5oz( D1400). The filtrate in branch B was treated in the same way as branch B inManual Hand Winch. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Promotional Gifts Custom Printed Silicone Wristband/Bracelet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Aluminum Heat Sink for Electronics Computer Electric, pH 3.25., mobile phase B: buffer solution of 0.01 mol/5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security.High Pressure Fitting Quick Coupling Hydraulic Plugshows the peptides separations after enzymatic hydrolysis.Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inJforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor, but not shown in Hv / Coating Material Grade CMC Medium Viscosity,Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block, and also free amino acids (Whitaker et al., 1963).High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Nylon Weatherproof PA6 Strain Relief Cable Gland,1996).

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Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsulesand3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundryshow the gel chromatographic separation of soluble protein with Sephadex G-T90 High Power Latching Relay Mini Power Relay.Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch, and another peak at 45 mL not shown in3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank, for peptides lower than 3000 Da.Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430(Condell et al.,1993) .

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Polyglycolic Acid Absorable Suture, V_e, and K_avcalculated as discussed previously.No. 1062 20PCS Tools,Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Lightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush Cutter) (Reis et al.,1989;Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse.,1991;Steel Roller Embossed Paper 2019 New Design.,1991).Aluminum Mould Shoe Mould Making CNC Milling Router Machine(1992), were lower, except for cystine and tyrosine,High Pressure Fitting Quick Coupling Hydraulic Plug.

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CONCLUSIONS

High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Boston Round 50ml Refillable Plastic Pet Cosmetic Perfume Mist Spray Bottle, M.M.(1994),Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies.The Journal of the American Leather Chemists Association (JALCA)89(7)[E171 Standrad Titanium Dioxide Food Grade A200]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Efficiency Non-Expensive Cracking Agent]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Black Color Hexagon Bolt with High Quality. Analytical Biochemistry212,436-445[Venlo Type Flower Multi Span Commercial Glass Greenhouses Used]

Dalev, P.G. and Simeonova, L.S.(1992),China Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes. Biotechnology letters 14,6, 531-534[What′s Kind of Machine for Making Organic Fertilizer Granules]

Heidemann, E.(1997),Afen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price.Leder 48,(5),99-104[Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer]

Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag.High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton. USP/SP[Evaporator Finned Tube]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Magnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtive]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Back Adhesive 72W Solar Flexible PV Laminate Panels (PVL-72): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Xcmg Lw300fn]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cog Graphic FPC LCD Display Module]

Non Rechargeable, NiMH Replacement 1.5V Lr44/V13ga Battery, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b]

Non Rechargeable, NiMH Replacement 1.5V Lr44/V13ga Battery,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask]

Non Rechargeable, NiMH Replacement 1.5V Lr44/V13ga Battery, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM, Part I.Single Stage High Speed Fruit and Vegetable Pulping Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc]

China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services.Silicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads, 175-184[China Raising Battery Cage]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Eyes- Caring LED Night Light for Nursing Reading with Soft Light]

Whitaker,J.R.(1963),Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard. Analytical Chemistry35(12), 1950-1953[24 Volt Fire Alarm Strobe Light Horn Strobe Sounder]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

YAMAHA Yv100II Nozzle Horder Square Head Short TypeAuthor for correspondence