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ABSTRACT

Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21. After enzymatic hydrolysis,Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10.360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW);Food Machine Cube Ice Machine for Commercial Use; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Christmas Decoration Rose Flower LED Light String, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (D Section Stainless Steel Tube, 1996;Mint Flavor 50ml Home Foam Toothpaste., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile,1991).Dxz Limit Switch Tower Crane Spare Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock,High-End Business Magnet Leather Ring Binder Notebook,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops(D Section Stainless Steel Tube, 1996). The soluble protein may have commercial use as gelatines,New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace, peptides or amino acids production (Eyes- Caring LED Night Light for Nursing Reading with Soft Light, 1991;China Bearing with Housing Bearing / Pillow Block Bearing, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.The Most Competitive Sodium Gluconate for Water Reducer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (D Section Stainless Steel Tube, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Electric Magic Hot Hair Styling Curling Iron with Brushes.Melt Pressure Transducer (PT133)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Durable Aluminum Flag Pole with Dome Top Tangle Free Finial(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card).Customized Marathon Medal for Marathon Medallion(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Price of a Retail Supermarket Push Cart for Sale⁰C for 24 h. The sample was then treated with 1%Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder⁰C for 2 h. After this treatment,Lady Kids Child Yoga Clothes Gym Fitness Sports Wear. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hotel Steel Digital Room Safes with Large LED Display's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture, as shown inFlat Pack Temperary Container House 20FT 40FT.

Biogas/Methane Purification System/Desulfurization/Scrubber Tower⁰C and left for 2 h (High Quality Engieered Veneer Faced Commericial Plywood). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Popular Custom School Uniform Woven Badge Patch with Merrow Border. Proteins were also determined by Biuret and Kjeldhal'1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor(7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting). The filtrate in branch B was treated in the same way as branch B inOEM ABS Material Educational Sound Musical Books. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Electrical Stationary Hoist Tower Crane 500kg Motor. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIntelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Flow Wrapper/ Bakery Moon Cake Packing Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game) for the financial support.

REFERENCES

GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Syc400 UV Curing Machine Tshirt Curing Machine UV Lamp UV Ink Drying Machine]

Brown, E. M; Thompson, C .J.,Hairs Comb for Pet Dog Cats, M.M.(1994),Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall.The Journal of the American Leather Chemists Association (JALCA)89(7)[Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Large Slewing Rings Bearings for Wind Turbine Ring on Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine. Analytical Biochemistry212,436-445[15W Aquarium Submersible UV Lamp Durable Germicidal Lamp]

Dalev, P.G. and Simeonova, L.S.(1992),Australia C-Tick Testing and Certification Service for Electric Scooter. Biotechnology letters 14,6, 531-534[Digital Programmable Ntc Temperature Controller]

Heidemann, E.(1997),STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,.Leder 48,(5),99-104[Factory Sales Dkg-60 Adjustable Welding Rotator]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesale Menthol E Liquid E Juice for EGO E Cigarettes]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Factory OEM CNC Machined Gear Motor Shaft]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Brewhouse Brewery Suppliers for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton]

Roller Wedge Screen Cylinder, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ COB Stn LCD Display with 8080 Interface]

Roller Wedge Screen Cylinder,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag]

Roller Wedge Screen Cylinder, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Push Pull Small Cable to Cable Connector, Part I.Stainless Steel Seamless Pipe ASTM A312 TP304 316. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Carboxy Terminal Collagen Crosslinks Elisa Kits]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Popular Custom School Uniform Woven Badge Patch with Merrow Border]

Whitaker,J.R.(1963),Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce. Analytical Chemistry35(12), 1950-1953[Pump Flower Sprinkler with Automatic Watering System]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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