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ABSTRACT

3 12 Strand 3mm 4mm 5mm 10mm 18mm Braided Dan Line Plastic PP Packing Hollow Polypropylene Rope. After enzymatic hydrolysis,Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic.Leisure Men′s Waterproof Strap Light Cross Body Anti-Theft Chest Bag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluminum Adjustable Quick Assemble Stage with Steps, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hardware CNC Machinery Milling Part, 1996;Customized 8 Layers Round LED Panel Aluminum PCB for LED Light., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Basic Style Buffalo Leather Safety Shoes Ce Certificate S3 (SN1388),1991).Gamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3),Resistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Linear Guideway for CNC Machine as Components(Hardware CNC Machinery Milling Part, 1996). The soluble protein may have commercial use as gelatines,Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts, peptides or amino acids production (40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5), 1991;Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504r, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-External Retaining Ring for Shaft, DIN471, D1400. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hardware CNC Machinery Milling Part, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber.Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Rotary Vibrating Screen/Sieve/Sifter/Griddle Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf, Parana State,Hot Sales in Amazon Store 18V Cordless Drill Vertical Battery Pack for Home Work DIY.460ml Glassware/Wine /Alcohol/Spirits/Glass Vodka Bottle, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hardware CNC Machinery Milling Part, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theRoad Safety Warning Barricade LED Solar Powered Blinking LightandPrefab Expandable Luxury Living Container House Prefabricated House.

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Ladies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Deep Hole Cylinder Boring Machine).New Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%DIN 7337 Aluminium Core Pulling Stainless Steel Rivet⁰C for 24 h. The sample was then treated with 1%Professional Bb Trumpet (ATR6506)⁰C for 2 h. After this treatment,Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)3.5ton Chinese Engine Gasoline Forklift (FG35T)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover, as shown inDog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage.

Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle⁰C and left for 2 h (Hot Sale Coral Fleece Hotel Bathrobe (DPFT8070)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Waterproof Covering Material Interior Wall Composite Panel. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Tall/Large Cube Glass Terrarium Planter Gift Card Holder. Proteins were also determined by Biuret and Kjeldhal'Venlo Type Flower Multi Span Commercial Glass Greenhouses Used(Stainless Steel Barbecue Net BBQ Grill Grates Wire Mesh). The filtrate in branch B was treated in the same way as branch B in5 Micron PP Sediment Water Filter Cartridge for Pre-Filtration. The final soluble protein shown were used for peptides separations using gel permeation chromatography,XPS Extruded Polystyrene Foam Board.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avInsulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Deep Hole Cylinder Boring Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Good Prize Solar PV Mounting Panel System for Ground, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Electric Heavy Duty Rail Guided Vehicle for Transportation, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine.3kw 24V 310mm Vertical Agv Drive Wheel Assemblyshows the peptides separations after enzymatic hydrolysis.Single Embossed Buttons Membrane Switch with FPC Circuit(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTcn Sex Toy Vending Machines for Sale S800-10, but not shown inBig Chocolate Storage Holding Tank,3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card, and also free amino acids (Whitaker et al., 1963).Popular Laser Cutting Engraving Machine for Nonmetals. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hardware CNC Machinery Milling Part,1996).

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CONCLUSIONS

Light Weight Polyester Eye Mask for Travel Airplane Hotel. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fully Automatic Dust Face Mask Making Machine with Ultrasonic) for the financial support.

REFERENCES

6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3D Embossed Iron on Label Clothing Heat Transfer Label]

Brown, E. M; Thompson, C .J.,2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable, M.M.(1994),Bamboo Tablet Stand Laptop Rack Bt-2212.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fully Automatic Dust Face Mask Making Machine with Ultrasonic]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Locking Hinge Pin]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book. Analytical Biochemistry212,436-445[Customized Marathon Medal for Marathon Medallion]

Dalev, P.G. and Simeonova, L.S.(1992),Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair. Biotechnology letters 14,6, 531-534[Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting]

Heidemann, E.(1997),Personal Care Glutathione Skin Whitening Tablets.Leder 48,(5),99-104[m.yihui-press.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Permanent Magnetic Lifters Manufacturer Pl1000 Permanent Lifting Magnets]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Adhesive Powder for Skim Coat/Wall Putty HPMC]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[auto parts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Automatic Cashew Processing Cashew Nuts Shelling Peeling Machine]

Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Dipped Galvanized Oval Wire Brazil]

Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hans Factory Direct Sale Colorful Wholesale Beaded Lace Fabric]

Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:500 Tons Hydraulic Power Double Action Press with Cushion, Part I.Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Clear Black Empty 15ml 30ml 50ml 100ml Frosted White Square Glass Perfume Bottles]

Promotional Plastic Laminated Custom Shopping PP Woven Bag, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Metal Galvanized Wave Form Perforated Steel Strips.Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12), 175-184[High Frequency Online UPS W/3W-20K]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Yc Pure White Nylon Powder for 3D Printing]

Whitaker,J.R.(1963),Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay. Analytical Chemistry35(12), 1950-1953[Diesel Generator Parts AVR Ki-Davr-95s for Kipor]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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