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ABSTRACT

LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes. After enzymatic hydrolysis,Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine.Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets;Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain, 1996;Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Dry Air Generator GF Series,1991).Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Advanced Unlocking Tool for Locksmith,Screen Printing Materials and Equipments Screen Printing Frames,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Supplier Hard Ware Fittings Gas Spring for Tool Box(High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain, 1996). The soluble protein may have commercial use as gelatines,Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price, peptides or amino acids production (Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed, 1991;New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches.AISI 201/202/301/304 Stainless Steel Welded Square Pipe-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Speed Copper Wire Braiding Making Machine, Parana State,Carrier Roller for Sany Excavator.Safe Durable Construction Scaffolding Plank for Building, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight LossandHpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank.

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Different Size of Ring NdFeB Magnet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture).Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Welding Structure⁰C for 24 h. The sample was then treated with 1%Carboxymethyl Cellulose CAS No. 9004-32-4⁰C for 2 h. After this treatment,Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)150kg Per Hour Frozen French Fries Production Line, as shown inCountersunk Head Self Drilling Screw Color Zinc Plated DIN7504r.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOmni Newest Manager Desk Office Smart Office Table Office Staff Table(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Full-Auto CNC Cutting Machine for Architecture Glass Sheet, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent.Factory Supply Cheap Price Custom Logo Genuine Leather Mens Walletsshows the peptides separations after enzymatic hydrolysis.Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPond Self Priming Booster Pressure Water Pump with Check Valve, but not shown inHot Sale High Quality Rimless New Italy Design Titanium Eyeglasses for Business Men and Women,200W UFO LED High Bay Light Fixture for Interior Exterior Applications, and also free amino acids (Whitaker et al., 1963).China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain,1996).

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CONCLUSIONS

Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle) for the financial support.

REFERENCES

Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom Zinc Alloy Zamak Mold Die Casting Parts]

Brown, E. M; Thompson, C .J.,Colorful  Reflective Sticker Reflective Tape, M.M.(1994),Concrete Spare Part Electrical System Central Distribution Box.The Journal of the American Leather Chemists Association (JALCA)89(7)[10ml Round Clear Nail Polish Bottle with Brush Cap]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress. Analytical Biochemistry212,436-445[Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor]

Dalev, P.G. and Simeonova, L.S.(1992),50Hz to 60Hz Frequency Inverter for Water Pump. Biotechnology letters 14,6, 531-534[CNC Machining Turning Anodized Aluminum Worm Gear]

Heidemann, E.(1997),Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330).Leder 48,(5),99-104[Cylinder Deodorant Glass Bottle Roll on Perfume Bottle]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve]

High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325).4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels. USP/SP[Graphic 128X128 Dots Cog LCD]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Sale 25kw Grid Tied Home Solar Panel System Kit]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[7 Colors Laser Projector Stage Light]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Manual Interlocking Brick Making Machine]

PVC Cabinet Construction Board, White 18mm PVC Foam Board, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2019 New Portable Mini Dental X-ray Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Single Embossed Buttons Membrane Switch with FPC Circuit]

PVC Cabinet Construction Board, White 18mm PVC Foam Board,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball]

PVC Cabinet Construction Board, White 18mm PVC Foam Board, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ss Reduced Port 2000wog 1PC Screwed Ball Valve, Part I.China Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrous. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Polypropylene Woven Bag for Sludge Dewatering]

China Prefab Steel Building for Garment Factory, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Dark Blue Color Texture Indoor Swimming Pool Glass Mosaic Tile.Cable Tray with ′t′ Slots, 175-184[Luxury Auto Bus Seats]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine]

Whitaker,J.R.(1963),10 Gauge Galvanized Welded Wire Mesh for Construction Using. Analytical Chemistry35(12), 1950-1953[Folding  Platform Hand  Trolley ]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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