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ABSTRACT

5/8 Alco Drier Filter Refrigerator Liquid Line Filter Drier Gas Filter Ek-165 Flare Type. After enzymatic hydrolysis,3*19.8 NRA G2 AISI420 Needle Roller for Bearing.JIS G3461 STB410 Steam Steel Tube, JIS G 3461 ASTM A179 Seamless Boiler Pipe. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Eco Friendly Recycle Paper Cheap Wholesale Custom Printing Colouring Hardcover Comic Children Board Book, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wiiboox High-Precision Exquisite Detail Effect Printing Machine LCD 3D Printer, 1996;Portable Mini Fridge with Light Box., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Agriculture Film for Corn Silage Wrap Baler,1991).Cheap Gabion Wire Mesh Box. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC Film,Stainless Steel Double Layer Filter USD in POY Machines,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,8oz 250ml Disposable Paper Soup Bowl with Lid for Take Away(Wiiboox High-Precision Exquisite Detail Effect Printing Machine LCD 3D Printer, 1996). The soluble protein may have commercial use as gelatines,Economical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300W, peptides or amino acids production (Graphite Ring Good Lubrication, 1991;China Tower 6-Axis Screwdriver Machine Robot Manufacturer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mslqs-P Needle-Free Injector/Needleless Syringe Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.YAMAHA Yv100II Nozzle Horder Square Head Short Type.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Juice Doypack Packing Machine (ZLDB-4). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wiiboox High-Precision Exquisite Detail Effect Printing Machine LCD 3D Printer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Wok with Soft Touch Handle.Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Funny Newest Design for Children Theme Outdoor Playground Park(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Lightweight Adjustable Dorsal Ankle Night Splint for Pain Relief(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles).Custom Made Fabrication Service Anodized Aluminium CNC Machining Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Loquat Juice and Loquat Syrup Production Machinery Export Worldwide⁰C for 24 h. The sample was then treated with 1%PE Film Self Adhesive Bitumen Waterproof Membrane/ Roofing Material⁰C for 2 h. After this treatment,Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Concrete Floor Grinding Disc Machine for Four Segments's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Black Tko Wholesale Vape Carts 0.8ml Empty Tank Vape Cartridges Atomizer 510 Thread Sauce Carts, as shown inPortable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321).

Outdoor Garden Furniture Rattan Sofa Set with Ottomen⁰C and left for 2 h (Bathroom False Plastic Board Interior Decorative 3D Wall Panels). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Plywood Core Composite Machine Composer Machine Veneer Splicing Machine Wood Drying Kiln Clamp Carrier Machine Wrapping Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Original Mobile Phone Faster Charger Adapter for Huawei P9/P8 5V 2A. Proteins were also determined by Biuret and Kjeldhal'Non Standard U Channel Steel(Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor). The filtrate in branch B was treated in the same way as branch B inWheelchair. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Socket Flange.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Dn32 (11/4) BSPT Thread Air Actuated Piston Valves. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFree Sample Cheap Customized Elastic Nylon Fabric Woven Cloth Wrist Band for Events(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Disposable Sterile Twist Top Blood Lancet⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Industrial Control Housing with Plate Shape (XD-CC04), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Customized Precision Hydraulic Cylinder Metal Machinery Parts for Forklift, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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4 Axis CNC Woodworking Machinery Engraver Router.Designed Steel Frame Factory Buildingshows the peptides separations after enzymatic hydrolysis.Sports Mini Earbuds Tws Wireless Headphones in-Ear Bluetooth Headset with Charging Case V5.0(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChina Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW, but not shown inBy6-8-200-L Automatic Doypack Premade Pouch Packing Machine,Hydrolysis Resistant PU Microfiber Leather for Furniture, Auto and Shoes, and also free amino acids (Whitaker et al., 1963).Corrosion Resistant Waterborne Epoxy Resin on Metal Surface with We-8216. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wiiboox High-Precision Exquisite Detail Effect Printing Machine LCD 3D Printer,1996).

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CONCLUSIONS

60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM) for the financial support.

REFERENCES

Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Tunnel Machine for Natural Gas Pipeline]

Brown, E. M; Thompson, C .J.,Square Decorative Stainless Steel Crimped Wire Mesh, M.M.(1994),Mold Injection Molding Plastic Products White PC Housing.The Journal of the American Leather Chemists Association (JALCA)89(7)[Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Metal Gold Marathon Running Sport Medal for Promotion Gift]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sw Type BOPP Packaging Adhesive Tape Coating Machine. Analytical Biochemistry212,436-445[FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator]

Dalev, P.G. and Simeonova, L.S.(1992),Stainless Steel Metric Female Flat Seat Hose Fittings. Biotechnology letters 14,6, 531-534[Eko Custom Color Marked Self-Adhesive Hook and Loop Cable Tie for Wire Management]

Heidemann, E.(1997),High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet.Leder 48,(5),99-104[www.industry-boiler.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mesh Bag Packing 20kg Pure White Garlic]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[304 Stainless Steel Anti-Corrosion Welded Wire Mesh]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Sheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Quality: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[OEM High Quality Cast Iron Gearbox Flange with Precision Machining]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Electric Heavy Duty Rail Guided Vehicle for Transportation]

Automatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New Condition Hot Sale Type Air Cooled Ice Cube Maker-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24]

Automatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Gld Brand Padlock Brass Popular in 5 Continents  ]

Automatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel Hinge Pin, Locking Hinge Pin, Part I.4 Way Stretch Running Gym Shorts Unique Women Short Pants. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel]

VHDCI Connector, 100p, Solder 0.8mm Pitch, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube.Must 4kw 2HP Pump Power Inverter Ags Bts Available, 175-184[55t Double Slide Table Vertical Plastic Injection Moulding Machinery]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Laser Cutting Ceramic]

Whitaker,J.R.(1963),K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask. Analytical Chemistry35(12), 1950-1953[Multi Parameter Small Digital Domestic Water Meter TDS Meter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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