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ABSTRACT

Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop. After enzymatic hydrolysis,High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng.Precision Customized OEM Auto Hardware Lathe CNC Machining Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;White Color Enamel Bowls with High Quality;Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board, 1996;Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Southeast Asia Healthy Soft Integrated Latex Foam Mattress,1991).12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Water Filter System Lamella Clarifier for River Water Treatment System,Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cabinet Shelf Support Pin Furniture Hardware Fittings(Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board, 1996). The soluble protein may have commercial use as gelatines,Reasonable Price High Quality Nylon Cable Gland, peptides or amino acids production (Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan, 1991;Disposable Anesthesia Breathing Circuit Tube, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks.Counter-Clip Fluorine-Lined Butterfly Valve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:DC Brushless Motor for CNC Machine with ISO9001(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Rectification Triming Metallized Polyester Film Slitting Rewinding Machine).Solar Energy Storad Power Generation System(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K)⁰C for 24 h. The sample was then treated with 1%Custom Aluminum Cover Frame Half Aluminum Cover Profile⁰C for 2 h. After this treatment,Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)DC24V 36W Solar Fan Motor PCBA Card's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New Monocular Hunting Wargame Mini Scope Digital Night Vision, as shown inPlug-in Supermarket Chest Type Display Freezer with Sliding Glass Door.

Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine⁰C and left for 2 h (New Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Italy Design Good Quality Hydraulic Motorcycle Lift Table. Proteins were also determined by Biuret and Kjeldhal'Wholesale Menthol E Liquid E Juice for EGO E Cigarettes(Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover). The filtrate in branch B was treated in the same way as branch B inFactory 3*24-24mm Combination Rope for Trawling/Mooring/Security Work. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPortable Telescopic Straw Metal Drinking Straw with Brush Case(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Etching Rear Trunk Sill Plates for Hyundai Tucson⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Quality Horizontal Flat Bed Metal CNC Turning Lathe Machine Price Ck6163, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Lab Equipment Sr Paper Pulp Beating Freeness Test Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262)andCREE COB Meanwell Driver Walkway Lamp 45W LED Canopy. Enzymatic (Car Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima)Popular Water Bike / Hydro Bike / Aqua Bike (SK-8001)(sulfuric acid)Fertilizer Granulator Machine, Compound Fertilizer & Organic Fertilizer Granulator, Machine for Making(Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Double Roller Rotary Wood Peeling Machine with CE for Sale, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Double Roller Rotary Wood Peeling Machine with CE for Sale, flow rate of 0.1 mL/min., and l = 620 nm.

5in1 USB Type C Hub HDMI 4K USB C Hub to Ethernet RJ45 LAN Adapter.Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Cardshows the peptides separations after enzymatic hydrolysis.Hot Sale Coral Fleece Hotel Bathrobe (DPFT8070)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFlat Pack Temperary Container House 20FT 40FT, but not shown inHigh Quality Engieered Veneer Faced Commericial Plywood,Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone, and also free amino acids (Whitaker et al., 1963).Ladle Refining Melting Steel Furnace Casting Ladle. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board,1996).

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Factory Custom Highly White Clear 750ml Tequila Liquor Glass Bottle, V_e, and K_avcalculated as discussed previously.Galvanized Eye Grab Bend Hook,Transparent LED Display P3.91 Glass Wall LED Screen. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price) (Reis et al.,1989;Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan.,1991;Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration.,1991).Surgical Power Tool Medical Care Orthopedic Mini Bone Saw(1992), were lower, except for cystine and tyrosine,Micro Small Plastic Product Injection Molding Making Machine.

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CONCLUSIONS

Glass Lifting Machine with Sucker for Glass Movements. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Manufacturer Customized Plastic Injection Molding) for the financial support.

REFERENCES

Anodized Gold Brush Finish Aluminum Sheet Coil, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[CNC Aluminium Die Casting 7075/6061/5051 Auto Parts]

Brown, E. M; Thompson, C .J.,Popular Industrial Sewing Quilting Machine With CO, M.M.(1994),Chinese Manufacturer Provide First-Class Service for Non Stretch/Elastic Allover Lace.The Journal of the American Leather Chemists Association (JALCA)89(7)[4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[3 12 Strand 3mm 4mm 5mm 10mm 18mm Braided Dan Line Plastic PP Packing Hollow Polypropylene Rope]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate. Analytical Biochemistry212,436-445[Australia C-Tick Testing and Certification Service for Electric Scooter]

Dalev, P.G. and Simeonova, L.S.(1992),Pure Sine Wave Power Frequency Inverter. Biotechnology letters 14,6, 531-534[Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints]

Heidemann, E.(1997),Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine.Leder 48,(5),99-104[China Makeup Mirror Led Lights]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Large Slewing Rings Bearings for Wind Turbine Ring on Sale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-33kv Dry Type Resin Casted Distribution Transformer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[15W Aquarium Submersible UV Lamp Durable Germicidal Lamp]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[CNC Mini CO2 Laser Cutting Machine for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Digital Programmable Ntc Temperature Controller]

Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Economic and Reliable P32 Hydraulic Hose Crimping Machine Italy with SGS Certificate-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element]

Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag]

Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Size 000 Clear Pullulan Vegetarian Capsules, Part I.Sinicline 2018 Hot Sell Custom Printing Thank You Cards. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Menthol E Liquid E Juice for EGO E Cigarettes]

Black Red Open Net Spandex Nylon Sexy Lingerie, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting.Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor, 175-184[High Precision Injection Pipe Tee Fitting Mould]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Factory OEM CNC Machined Gear Motor Shaft]

Whitaker,J.R.(1963),LED Emergency Exit Lights. Analytical Chemistry35(12), 1950-1953[High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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