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ABSTRACT

Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616). After enzymatic hydrolysis,10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light.Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;ABC-508 Brake System Cleaning and Fluid Exchanging Machine;Polypropylene Woven Bag for Sludge Dewatering; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing, 1996;High Quality Industrial Grade Anhydrous Sodium Sulfate., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Professional Factory Supply Design Cat Tree Pet Product,1991).Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Peb Steel Structure Building Materials H-Section I Beam Steel,Aluminum Oxide Non Woven Polishing Wheel for Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CAT6A FTP Patch Panel (WD6A-012)(High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing, 1996). The soluble protein may have commercial use as gelatines,LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine, peptides or amino acids production (Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant, 1991;Famous Brand Luxury Packaging Box Gift Box, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Qualified Medical Grade Thin Wall Stainless Steel Tube., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Strength New 0.18mm Molybdenum Wire. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Structural Synthetic Fiber for Concrete.LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth, Parana State,Fashion Metal Button Crystal Shank Button for Garment.AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theI12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs MaxandLiving Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa.

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34*25cm Office File Pocket Felt A4 Document Bag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type).PP Needlepunched Nonwoven Geotextile(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Temperaturethermocouple Male and Female Plug⁰C for 24 h. The sample was then treated with 1%34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts⁰C for 2 h. After this treatment,Best Price Custom Embroidery Logo Hotel Slippers. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Efficiency Fine Powder Vibrating Screener, as shown inStainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%UF Membrane Aqu160 with Competitive Price and High Quality. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAtf-805 Auto Car Transmission System Flushing Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Precision C45 Steel CNC Lathe Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Aluminum Adjustable Quick Assemble Stage with StepsandCAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate. Enzymatic (Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1)Food Grade PTFE Coated Fiberglass Mesh Baking Sheet(sulfuric acid)Beverage Cooling Cube Ice Making/Ice Maker Machinery(High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank, flow rate of 0.1 mL/min., and l = 620 nm.

Transparent Composite Fiberglass Roof Tiles.Industrial Control Housing with Plate Shape (XD-CC04)shows the peptides separations after enzymatic hydrolysis.Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in DIN914, but not shown inCar Air Conditional Compressor Magetic Clutch 5pk,Two Layer License Plates with Wide Border Embossed, and also free amino acids (Whitaker et al., 1963).Electrical Barber Shop Equipment Electric Hair Curling Wand Iron. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing,1996).

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CONCLUSIONS

Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet) for the financial support.

REFERENCES

Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Mini Photo Eye Sensor Supplier & Factory]

Brown, E. M; Thompson, C .J.,Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness, M.M.(1994),LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light.The Journal of the American Leather Chemists Association (JALCA)89(7)[Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Chinese 1325 Ce Certificated CNC Engraving Router for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Safest Baby Healthcare and Grooming Kit for Baby Gift. Analytical Biochemistry212,436-445[ Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights]

Dalev, P.G. and Simeonova, L.S.(1992),Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby. Biotechnology letters 14,6, 531-534[Amazon Pet Supplier Mesh Dog Chest Back Traction Rope Aliexpress Set Dog Harness Dog Leash]

Heidemann, E.(1997),Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro.Leder 48,(5),99-104[www.dgls-tech.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Apparel Applique Round Patch School Club Embroidery Collar Patch]

Braiding Machine for Weaving Flexible Metal Hose, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Micro DC 24V Worm Gear Brushless Motor for Medical Equipment.1.6 Ton Hand Lifting Tool Lever Chain Block. USP/SP[Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[DC Brushless Motor for CNC Machine with ISO9001]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-CNC Machining Service High Precision CNC Mechanical Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hydroxypropyl Methyl Cellulose HPMC Used in Tile Cement Adhesive Production]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Lcd Glue Remover]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine]

Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear]

Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom ABS&POM&PP&PC&Acrylic Plastic CNC Machining Turning Parts]

Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Printed 100% Polyester Tcheap Wholesale Blankets, Part I.Food Grade Material 304/316 Double Diaphragm Pumps. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Mini Small Garden Excavator Digging Work]

Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator.High Power From Han′s GS 2000W Laser Cutter, 175-184[400mm Indoor Decorations Waterproof and Fireproof 3D Color Series PVC Wall Panel]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light]

Whitaker,J.R.(1963),Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel. Analytical Chemistry35(12), 1950-1953[Disposable Anesthesia Breathing Circuit Tube]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

DC 12V 24V Mini Portable Car Refrigerator Compressor FridgeAuthor for correspondence